Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM15,737,7560AG33.2% 316.5 / 104.6 277intergenic (+45/+26)hutF CDS (probable formiminoglutamate deiminase)/PP_5037 CDS (lipocalin family lipoprotein)–/–
Reads supporting (aligned to +/- strand):  ref base A (57/128);  new base G (1/91);  total (58/219)
Fisher's exact test for biased strand distribution p-value = 1.18e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 284 total aligned reads displayed.

GGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCT‑GAGCGTACCGGGGTTG‑CGTTGCAGCCCTTCGCG‑TGCAAGCCCGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAG  >  AE015451_phaM1/5737622‑5737892
                                                                                                                                         |                                                                                                                                         
gggCAATGGGTGGTGCGCAAGGGGCGGCATGCCCAGGAAGATGAAAGTGGGCGGGCGTTTACCAAGGTGTTGCGCAAGCTTTTGGGCT‑GAGCATACCGGGGTTG‑CGTTTACGCCCCTCGCA‑TGCAAGCCCGCGCAgg                                                                                                                                         >  2:14130549/1‑137 (MQ=255)
  gCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCT‑GAGCGTACCGGGGTTG‑TCTTTCCGCCCTTCCCT‑TGTAAAACCCCGAAAGGt                                                                                                                                       >  2:19111073/1‑137 (MQ=255)
  gCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCT‑GAGCGTACCGGGGTTG‑CGTTTCACCCCTTCTCG‑TGCCAACCCGCCAAGGGt                                                                                                                                       >  2:19234775/1‑137 (MQ=255)
         ttggtgCTCCAGGTTCGGCATTCCCAGGAAGATAAAAGCGGGCGGTCGTTTGCCAAGTTTTTGCTCCAGCTTTTGGGCT‑GAGCGTACTGTGGTTG‑CGTTGCAGCCCTTCGCG‑TGCAAGCCCTCGAAGGGTTgca‑acg                                                                                                                               <  2:4173455/136‑1 (MQ=255)
              gcgcCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCT‑GAGCGTACCGGGGTTT‑TGTTTTAGCCCCTCCCG‑TGTCAGACCCCGAAGGGTTTCC‑AAGCAAcc                                                                                                                          >  2:8977243/1‑137 (MQ=255)
               cgcCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCT‑GAGCGTACCGGGGTTG‑TGTTTCCACCCCTCGCG‑TGCCAAACCACAAAAGGTTGCC‑ACACAAacc                                                                                                                         >  2:9373865/1‑137 (MQ=255)
                       gcggcATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCT‑GAGCGTACCGGGGTTG‑GGTTGGAACCCCCCTCG‑TGAAAGCCCCCGAAAAGATTCA‑AACAAAACCCaaaaaaa                                                                                                                 >  2:7354101/1‑136 (MQ=255)
                        cggcGTGCCCAGGAAGAGGAAAGCGGGCGGCCGTTTACCAAGGTTTTGCGCCATTTTTTGGGCT‑GAGCGTACCGGGGTTG‑TGTTGCAGCCCTTCGTG‑TGCAAGCCCGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACt                                                                                                                <  2:14124749/137‑1 (MQ=255)
                        cggcATGCCCAGGAAGGTGAAAGCGGGAGGGCGTTTGCCAAGGTGTTGCGCCAGTTTGTGGGCG‑GAGTGTACTGGGGTTG‑TTTTGCAGCCCTTCGCG‑GGCAAGCCCGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACt                                                                                                                <  2:15130989/137‑1 (MQ=255)
                        cggcATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCT‑GAGCGTACCGGGGTTG‑CATTGCAGCCCTTCGCG‑TGCAAGCCCGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACt                                                                                                                <  2:3990778/137‑1 (MQ=255)
                        agtaaTGCCCATGGAGGTGAAGGTGGGTGGGTGTTGACAGAGGTGTTGCGCCAGTTTTGGGGCG‑GATCGTCCCGGGGTTG‑CGTGGCAGCCCTGCGTG‑GGCAAGCCCGCGGGGGGTTACA‑ACGCAACCCCAAAAAACt                                                                                                                <  2:7561307/133‑1 (MQ=255)
                          gcATGCCAAGGAAGATAAAAGTGGGTGGGCGTTTGCCAAGGTGCTGCTCCAACTTTTGGGCT‑GAGCGGACCGGGGTTG‑CGTTGCAGCCCTTCGCG‑TGCAAGCCCGCGAAGGGGGGAA‑ACGCAACCCCAAAAAACTCa                                                                                                              <  2:17927398/137‑1 (MQ=255)
                            aTGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCT‑GAGCGTACCGGGGTTGTGGTTTCAACCC‑TCACGTTGAAAGCCCAAGAGGAGTTGCACACGCACCCCCCAAAAAcaaa                                                                                                              >  1:18681728/1‑134 (MQ=255)
                               cccAGAAAGATGAAAGCGGGCGGACTTTGGCCAAGGTGTTGCACCAGCTTTTGGGCT‑GAGCGTACCGGGGTTG‑CGTTGCAGCCCTTCGCG‑TGCAAGCCTGCGAGGGGTTGAA‑ACGCAACCCCAAAAAACTCAGTGCa                                                                                                         <  2:6699627/137‑1 (MQ=255)
                                 aaGGAAGATAAAAGCGGGCGGTCGTTTGCAAGGGTTTTGCTCCATTTTTTGGGCG‑GAGCGTACCGGGGTTG‑CGTTGCACCCCTTCGCG‑TGAAATCCCGCGAGGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCAtt                                                                                                       <  2:14424880/136‑1 (MQ=255)
                                   ggAAGATGAAAGCGGGCGGGCGTTTGCCAAGTTGTTGCGCCAGCTTTTGGGCT‑GAGCGTACCGGGGTTG‑CGTTGCATCCCTTCGCG‑GGCAAGCCCGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCAtttt                                                                                                     <  2:18082962/137‑1 (MQ=255)
                                    gAAGATGAAAGTGGGCGGGCGTTTGCAAGGGTGTTGCCCCAGCTTTGGGGCT‑GAGCGTACCGGGGTTG‑CGTTGCAGCCCTTCTTG‑TGAAAGCCCGCGAGGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCAttttt                                                                                                    <  2:8610352/137‑1 (MQ=255)
                                    gAAGAGGAAAGTGGGGGGGTGTTTGCAAAGGTGTTGCACCAGTTTGGGGGTG‑GAGCGTACGGGGGTTG‑TGTTACAGCCCTTCGCG‑GGAAAGCCCGAGAAGGGTGGAA‑ACGCAACCCCAAAAAACTCAGTGCAttttt                                                                                                    <  2:15267033/137‑1 (MQ=255)
                                         ggAAAGTGGGGGGGTGTTGGAAAAGGTGTTGCG‑CGGGTTTTGGGTTGGAGTGCCCTGGGGTGG‑TGTTACACCCTTTCGTG‑GAAAATCCGGCGAGGGGTGAAA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTAt                                                                                               <  1:15280702/136‑1 (MQ=255)
                                               tgtgcgggcgTTTGCCAAGGTTTTTTGCCAGCTTTTGGGCT‑GGGTGTACTTGGGTTG‑CGTTGCAGCCCTTTGTG‑GGAAACCTTGTGAAGGGTGGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTg                                                                                         <  1:16964309/134‑1 (MQ=255)
                                                    ggttttttGCCAGGTTGTTGCGCCATTTTTTGGGCT‑GATCGTACTGGGGTTG‑TGTTGCATCCCTTCGCT‑TGCAATCCCGTGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCgg                                                                                    <  2:13507623/132‑1 (MQ=255)
                                                    gggagTTTGCAGAGGTTTTGTGCCAGTTTGGGGGTG‑GGGTGTATAGGGGTTG‑CGTTGAAGCTTTTTGGG‑GGAAAGCCAGGGGGGGGGGGAA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCgg                                                                                    <  1:8197497/137‑1 (MQ=255)
                                                                gggTGTTGCACCAGTTTTTGGTTG‑GAGCGTACTGGGGTGG‑TGTTGAAGCCCTTTGCG‑TGAAAGCCCGCGAAGGGTGGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGa                                                                        <  2:11617362/136‑1 (MQ=255)
                                                                aGGTGTTGCGCCAGTTTTTGGGCT‑GAGCGTACCGGGGTTG‑TGTTGCAGCCCTTCGCG‑TGCAAGCCCGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGa                                                                        <  2:11842457/137‑1 (MQ=255)
                                                                  tttttGCGCCATCTTTTGGGTT‑GAGCATACCGGGGTTG‑CGTTTCACCCCTTCTCG‑TGCAAGCCCGCGAAGGTTTGCA‑ACACAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATc                                                                      <  2:4780756/134‑1 (MQ=255)
                                                                      ttcgcCAGCTTTGGGGCT‑GAGTGTACTGTGGTTG‑TGTTGCAGCCTTTTGCG‑GGAAAGCCCGCGAGGGGTGGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                                  <  2:12213817/135‑1 (MQ=255)
                                                                      tGCGCCATCTTTTGGTTT‑GAGTGTACTGGGGTGG‑CGTTGCAGCCCTTCGCG‑TGCAATCCCGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCATTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                                  <  2:17097609/137‑1 (MQ=255)
                                                                      tGCGCCAGTTTTTGGGTG‑GAGTGGATTGGGTTTG‑TGTTGCAGCTTTTCGTG‑TGCAGTCCCGCGAGGGGGTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                                  <  1:19345448/137‑1 (MQ=255)
                                                                      tGCGCCAGTTTTTGGGCT‑GGGCGTACCGGGGTTG‑CGTGGCAGCCCTTCGCG‑TGCAAGCCCGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                                  <  2:14532747/137‑1 (MQ=255)
                                                                      tGCGCCAGTTTTTGGGCG‑GAACGTCCCGGGGTTG‑CGTTGCAGCCTTTCGCG‑GGCAAGCCCGCGAAGGGTGGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                                  <  2:16263260/137‑1 (MQ=255)
                                                                      tGCGCCAGCTTTTGGGCT‑GAGCGTACCGGGGTGG‑CGTTGCAGCCCTTCACG‑TGCAAGCCCGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                                  <  2:12938644/137‑1 (MQ=255)
                                                                      tGCGCAATCTTTTGGGCG‑GAGCGTACGGGGGTTG‑TGTTGAAGCCTTTCGCG‑GGAAAGCCCGAGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                                  <  2:12390473/137‑1 (MQ=255)
                                                                      tGCACCAGCTTGTGGGTT‑GAGCGTACCGGGGTTG‑CGGTGAAACCCTTCGCG‑TGCAAGCCCGAGAAGGGTGGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                                  <  2:16989421/137‑1 (MQ=255)
                                                                      ccccccAGCTTTGGGGCT‑GAACGTACCGGGGTTG‑CGTTGCAGCTCTTTGCG‑GGAAAGCCCGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACGCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                                  <  1:13915747/133‑1 (MQ=255)
                                                                       gccccAGTTTTTGGGTG‑AAGTGTCCTGGGGTTG‑CGTTACAGCCTTTCGCG‑TGCAATCCCGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCg                                                                 <  2:7968383/134‑1 (MQ=255)
                                                                        ctccAGTTTTTGGGCT‑GATCGTACTGGGTTTG‑TGTTGCATCCCTTCGCG‑GTCAATCCCGCGAAGGTTTGAA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCgg                                                                <  1:11544593/135‑1 (MQ=255)
                                                                        ctccAGCTTTTGGGCT‑GAGCGTACCGGGGTTG‑CGTTGCAGCCTTTCGCG‑TGCAAGCCCGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCgg                                                                <  2:16569887/135‑1 (MQ=255)
                                                                        cgcCATTTTTTGGGTT‑GAGCGTACTGGGGGTG‑CTTTGCAGCCCTTCATG‑GGCAAGCCCGCAAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCgg                                                                <  2:12693000/137‑1 (MQ=255)
                                                                         gccggttttgGGGCG‑GAGCGGACCGGGGTTG‑TGTTGCAGCCTTTCTCG‑TGAAATCCCGCAAGGGGTGACA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGc                                                               <  1:19453937/131‑1 (MQ=255)
                                                                         gcCAGTTTGGGGGCG‑GAGAGTATAGGGGTTG‑CGTTGAAGCCCTGCGCG‑GAAAAACCAAAGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGc                                                               <  1:18080217/137‑1 (MQ=255)
                                                                         accATCTTTGGGGCG‑GAGCGTACTGGGTTTA‑TGTGACACCCCTTCGAG‑TGAAAGCCCGAGAAGGTTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGc                                                               <  1:17549861/136‑1 (MQ=255)
                                                                         accAGTTTTGGGGCT‑GATTGTACTGGGGTTT‑TGTTGCAGCTTTTTGTG‑TGAAGACCCGAGAGGGGTTGAA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGc                                                               <  1:4683577/136‑1 (MQ=255)
                                                                                      cT‑GAGTGTACTGGGTTTG‑TGTTGCACCCCTTCGTG‑TGCAAGCCCGCGAGGGTTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATAccc                                                  <  1:14375677/137‑1 (MQ=255)
                                                                                            tgTACGGGGGTTG‑TGTGGCAGCCCTTCGTG‑TGCAAGCCTGAGAGGGGTTACA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  2:5701949/136‑1 (MQ=255)
                                                                                            ggtaagggggTTG‑TGTTAAAGCCTTTCGTG‑GGAAAGCCCGAGAGGGGTTAAA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  1:9281553/131‑1 (MQ=255)
                                                                                            gggcccGGGGTTG‑TGTGGAATCCTTTCGGG‑GACAATCCCGCGAGGGGTGAAA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  1:7876342/133‑1 (MQ=255)
                                                                                            cGTACTGGGGTTG‑CGTTGCAGCCTTTCGCG‑TGCAAGCCCTCGAAGGGTTGAA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  2:4613537/137‑1 (MQ=255)
                                                                                            cGTACCGGGGTTG‑GGTGGCGGCCTTTCGCG‑TGCAAGCCTGCGAGGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  1:18379679/137‑1 (MQ=255)
                                                                                            cGTACCGGGGTTG‑CGTTGCAGCCCTTCGCG‑TGCAAGCCCGCGAAGGGTGGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  2:16767019/137‑1 (MQ=255)
                                                                                            cGTACCGGGGTGG‑CGTTGCAGCCCTTTGCG‑TGCAAGCCAGCAAAGGGTGGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  2:17416631/137‑1 (MQ=255)
                                                                                               actggggGTG‑TGTTGAAGCCCTTTTCG‑TGCAGCCCGGCGGGGGGTGAAA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGcc                                          <  1:12152242/134‑1 (MQ=255)
                                                                                               aCCGGGTTTG‑CGTTGCATCCCTTTGCG‑TGCAAGCCCGAGAGGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGcc                                          <  2:668595/137‑1 (MQ=255)
                                                                                               aCCGGGGTTG‑CGTTGCAGCCCCTCGCG‑TACAATCCCTCGAAGGTTTACA‑ACGTAACCCCAAAAAACGCAGTGCAGTTTGACTATTTGCTAGTCGGACGCCCGCCAGATCAAGCGGCTGGGGTCATACCCCCGCTGcc                                          <  2:19480545/137‑1 (MQ=255)
                                                                                                 tggggTTG‑TTTTGCATCCTTTCGTG‑TGAAAGCCCGAGAGGGGTTACA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCcgc                                        <  2:9817157/136‑1 (MQ=255)
                                                                                                 tggggTTG‑TTTTGAATCCTTTCGTG‑TGCAGTCCCTTGAGGGTTTACA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCcgc                                        <  1:13181544/136‑1 (MQ=255)
                                                                                                 cGGGGTTG‑CGTTGCAACCTTTTGTG‑GGAAATCCCAAGAAGGGTGAAA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCcgc                                        <  1:10223675/137‑1 (MQ=255)
                                                                                                  ggggTTG‑TGTTGCATCCTTTCGCG‑TGCAAGCCCGCGAGGGTTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCcgcg                                       <  2:18557497/137‑1 (MQ=255)
                                                                                                  ggggTTG‑CTTTGCATCTTTTTGCG‑GGAAAGCCCATGAAGGTTTACA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCcgcg                                       <  1:524790/137‑1 (MQ=255)
                                                                                                   gggTTG‑TGTTGCAGCCCTTCGTG‑TGCAATCCCGTGAGGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCgcgc                                      <  1:8723561/137‑1 (MQ=255)
                                                                                                      ttttttttgcATCCCTTTGGG‑TGAAAGCCCGCGAGGGGTGGAA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGccc                                    <  1:3233000/131‑1 (MQ=255)
                                                                                                      ttg‑cgttgcACCCTTTCGCG‑GAAAACCCCGGGAGGGGTTAAA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCt                                   <  1:10460159/137‑1 (MQ=255)
                                                                                                                aacccTTCGCG‑TGCAAGCCCGCGAAGGGTGGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagcag                          <  2:9708529/135‑1 (MQ=255)
                                                                                                                aGCCTTTCGTG‑GGCAAGCCCGGGAGGGGTGGAA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagcag                          <  2:6430006/137‑1 (MQ=255)
                                                                                                                  cccTTCGCG‑TGCAAGCCCGCGAAGGGTTGCA‑ACGCAAACCCAAAAAAATCAGTGTATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCACACCCCTGCTGCCGCGCCCCGGACAGCAGGt                        >  2:17824245/1‑137 (MQ=255)
                                                                                                                  cccTTCGCG‑TGCAAGCCCGCGAAGGGTTGCA‑ACACAACCCCAAAAAACCCCGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAACGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGt                        >  2:1849202/1‑137 (MQ=255)
                                                                                                                    ttttgtg‑TGCAGGCCCGTGAGGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtc                      <  1:1419492/131‑1 (MQ=255)
                                                                                                                    ttttgcg‑TGCAAGCCCGAGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtc                      <  1:8544828/133‑1 (MQ=255)
                                                                                                                    cTTCGCG‑TGCAATCCCGCGAGGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtc                      <  2:7082756/137‑1 (MQ=255)
                                                                                                                    cTTCGCG‑TGCAAGCCCGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtc                      <  2:3510374/137‑1 (MQ=255)
                                                                                                                       cgcg‑TGCAAGCCCGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCa                   >  1:5916984/1‑137 (MQ=255)
                                                                                                                        ccg‑TGCAAGCCCGCGAGGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCAc                  <  2:8171098/136‑1 (MQ=255)
                                                                                                                          g‑TGCAAGTGCGCGAAGGGTTGTC‑ACTCAACCCCCAAACACTCAGTGCATTTTCCCTCTTTGCTCCCCGGACGCCCACCAGATAAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCACTGGAACGCAGGTCCTCAcac                >  2:15690259/1‑135 (MQ=255)
                                                                                                                          g‑TGCAAGCCCGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACt                                                                                                                <  2:13082103/41‑1 (MQ=255)
                                                                                                                          g‑TGCAAGCCCGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGTAGGTTCTCACGc                >  2:12046379/1‑137 (MQ=255)
                                                                                                                          g‑TGCAAGCCCGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                >  2:14663284/1‑137 (MQ=255)
                                                                                                                          g‑TGCAAGCCCGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                >  1:2750004/1‑137 (MQ=255)
                                                                                                                          g‑TGCAAGCCCGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                >  1:12207548/1‑137 (MQ=255)
                                                                                                                          g‑TGCAAGCCCGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                >  2:2920004/1‑137 (MQ=255)
                                                                                                                          g‑TGCAAGCCCGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                >  1:6544581/1‑137 (MQ=255)
                                                                                                                          g‑TGCAAGCCCGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                <  2:18839884/137‑1 (MQ=255)
                                                                                                                          g‑TGCAAGCCCGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                >  2:12443855/1‑137 (MQ=255)
                                                                                                                          g‑TGCAAGCCCGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                >  1:9699385/1‑137 (MQ=255)
                                                                                                                          g‑TGCAAGCCCGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                >  2:1017281/1‑137 (MQ=255)
                                                                                                                           ttccAAGCCCGCGAGGGGTTACA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTGGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                <  2:433475/134‑1 (MQ=255)
                                                                                                                                   cctcgAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  2:2247473/134‑1 (MQ=255)
                                                                                                                                   ccaagAAGGGTTGCA‑ACGCAACCCCAAAAAACTCATTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  2:10473018/133‑1 (MQ=255)
                                                                                                                                   ccGGGAAGGGTTGAA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  1:8472457/137‑1 (MQ=255)
                                                                                                                                   ccGCGAGGGGTTGAA‑CCGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  1:11121150/137‑1 (MQ=255)
                                                                                                                                   ccGCGAGGGGTTGAA‑ACGCAACCCCAAAAAACTCATTGTATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  2:15150385/137‑1 (MQ=255)
                                                                                                                                   ccGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        >  2:14310816/1‑137 (MQ=255)
                                                                                                                                   ccGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  2:10979971/137‑1 (MQ=255)
                                                                                                                                   ccGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  2:7077519/137‑1 (MQ=255)
                                                                                                                                   ccGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        >  1:16469357/1‑137 (MQ=255)
                                                                                                                                   ccGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  1:6797733/137‑1 (MQ=255)
                                                                                                                                    cgcgAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGNATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAACAGGTTCTCACGCTCCCAGGcc       >  1:18819920/1‑137 (MQ=255)
                                                                                                                                    cgcgAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGcc       >  1:13007216/1‑137 (MQ=255)
                                                                                                                                    cgcgAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGcc       >  2:17886405/1‑137 (MQ=255)
                                                                                                                                     gcgAGGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCg      <  1:15830261/137‑1 (MQ=255)
                                                                                                                                     gcgAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCg      <  2:15915575/137‑1 (MQ=255)
                                                                                                                                         aGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAg  >  2:11313974/1‑137 (MQ=255)
                                                                                                                                         |                                                                                                                                         
GGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCT‑GAGCGTACCGGGGTTG‑CGTTGCAGCCCTTCGCG‑TGCAAGCCCGCGAAGGGTTGCA‑ACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAG  >  AE015451_phaM1/5737622‑5737892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑