BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	1,713,060	A→G	T2A (ACC→GCC)	tsaB CDS (endopeptidase) →

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	1,713,060	0	A	G	99.9%	3465.5 / NA	850	T2A (ACC→GCC)	tsaB CDS (endopeptidase)
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (470/379); minor base T (0/1); total (470/380)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 854 total aligned reads displayed.

GACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGACCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGCAAC  >  AE015451_phaM1/1712928‑1713192

gACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGgccac                                                                                                                                   >  2:2463727/1‑137 (MQ=255)

 aCGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGgccacc                                                                                                                                  <  1:8647532/137‑1 (MQ=255)

  cGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACNCAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCAccc                                                                                                                                 <  1:16784077/137‑1 (MQ=255)

  cGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCAccc                                                                                                                                 <  1:4921636/137‑1 (MQ=255)

  cGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCAccc                                                                                                                                 <  1:14399390/137‑1 (MQ=255)

  cGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCAccc                                                                                                                                 <  1:12348263/137‑1 (MQ=255)

       cTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCctgctg                                                                                                                            <  1:5804906/137‑1 (MQ=255)

        tCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATGCCCGTTCGATGGCCACCCTGCTgg                                                                                                                           <  2:8453386/137‑1 (MQ=255)

              aCGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTgg                                                                                                                     >  2:18707481/1‑137 (MQ=255)

                 gCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATa                                                                                                                  <  2:12342608/137‑1 (MQ=255)

                  cccGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATAc                                                                                                                 >  1:4395391/1‑137 (MQ=255)

                  cccGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCATCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATAc                                                                                                                 >  2:17900852/1‑137 (MQ=255)

                     gCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGc                                                                                                              >  1:6972969/1‑137 (MQ=255)

                      cTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGcc                                                                                                             <  1:17562929/137‑1 (MQ=255)

                         gCGTGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCAcc                                                                                                          <  2:14161201/137‑1 (MQ=255)

                         gCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCAcc                                                                                                          >  1:10324477/1‑137 (MQ=255)

                         gCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCAcc                                                                                                          <  1:3321985/137‑1 (MQ=255)

                          cGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCg                                                                                                         >  1:11833002/1‑137 (MQ=255)

                               cGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGcc                                                                                                    >  1:538351/1‑137 (MQ=255)

                               cGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGcc                                                                                                    >  1:11168320/1‑137 (MQ=255)

                                    tCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTc                                                                                               >  1:10662361/1‑137 (MQ=255)

                                      gTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCg                                                                                             <  2:15409193/137‑1 (MQ=255)

                                         tGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTcg                                                                                          <  2:8004814/137‑1 (MQ=255)

                                         tGTAGGCCGCACAGCGCCCCCGCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTcg                                                                                          <  1:11471914/137‑1 (MQ=255)

                                            aGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCgcgc                                                                                       >  2:11185919/1‑137 (MQ=255)

                                                cGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCgctgct                                                                                   <  2:2656785/137‑1 (MQ=255)

                                                cGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCgctgct                                                                                   <  1:9682410/137‑1 (MQ=255)

                                                cGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCgctgct                                                                                   <  2:11795343/137‑1 (MQ=255)

                                                cGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCgctgct                                                                                   <  2:6442970/137‑1 (MQ=255)

                                                 gCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGctgctg                                                                                  >  1:10101594/1‑137 (MQ=255)

                                                 gCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGctgctg                                                                                  >  1:6082066/1‑137 (MQ=255)

                                                 gCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGctgctg                                                                                  >  2:13880444/1‑137 (MQ=255)

                                                 gCACAGCGCCCCATCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACATGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGAATACCGCCACCGAAGCCTGTTCCGTCGCGctgctg                                                                                  >  2:8844440/1‑137 (MQ=255)

                                                     aGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATg                                                                              >  2:1290749/1‑137 (MQ=255)

                                                     aGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATg                                                                              <  2:10000142/137‑1 (MQ=255)

                                                     aGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATg                                                                              >  2:4632022/1‑137 (MQ=255)

                                                     aGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATg                                                                              >  1:16460143/1‑137 (MQ=255)

                                                     aGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATg                                                                              >  1:19453747/1‑137 (MQ=255)

                                                     aGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATg                                                                              >  1:2233206/1‑137 (MQ=255)

                                                     aGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCACTGGATACCGCCAACGAAGCCTGTTCCGTCGCGCTGCTGCATg                                                                              >  1:12644087/1‑137 (MQ=255)

                                                        gcCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACg                                                                           <  2:1102566/137‑1 (MQ=255)

                                                          ccccTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACgg                                                                          <  1:15562700/136‑1 (MQ=255)

                                                          ccccTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGc                                                                         >  1:14904638/1‑137 (MQ=255)

                                                              tCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAgg                                                                     <  1:371199/137‑1 (MQ=255)

                                                              tCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAgg                                                                     <  2:13202957/137‑1 (MQ=255)

                                                               cAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGt                                                                    <  2:4214506/137‑1 (MQ=255)

                                                               cAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGt                                                                    <  1:8942411/137‑1 (MQ=255)

                                                               cAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGt                                                                    >  2:7324469/1‑137 (MQ=255)

                                                                  gACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGAc                                                                 <  2:13599483/137‑1 (MQ=255)

                                                                   acacAACGTCGCTGCTGCTCTATACTGCCGCTCTTTTTCAGTCGCACCTTGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCGGCAGGACGGCAAGGTGAcc                                                                <  1:2798102/137‑1 (MQ=255)

                                                                   acacAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGAcc                                                                <  1:722236/137‑1 (MQ=255)

                                                                   acacAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGAcc                                                                <  2:15898481/137‑1 (MQ=255)

                                                                    cacaACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCa                                                               >  1:1524763/1‑137 (MQ=255)

                                                                        aCGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATGCCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCa                                                           <  1:3998615/137‑1 (MQ=255)

                                                                        aCGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCa                                                           <  1:456932/137‑1 (MQ=255)

                                                                          gccgcTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACt                                                         >  2:10536349/1‑137 (MQ=255)

                                                                              ctgctgctCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGa                                                     <  1:12866502/137‑1 (MQ=255)

                                                                               tgctgctCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAg                                                    >  2:16374862/1‑137 (MQ=255)

                                                                                 ctgctCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGt                                                  >  2:671735/1‑137 (MQ=255)

                                                                                  tgctCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTg                                                 <  1:7810638/137‑1 (MQ=255)

                                                                                    ctctATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAt                                               >  2:5222635/1‑137 (MQ=255)

                                                                                     tctATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑c                                             <  2:16871835/137‑1 (MQ=255)

                                                                                       tataCTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑ccc                                           >  2:11470005/1‑137 (MQ=255)

                                                                                       tataCTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑ccc                                           <  2:2129759/137‑1 (MQ=255)

                                                                                            tGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTTGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCgca                                      >  1:6467710/1‑137 (MQ=255)

                                                                                            tGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCgca                                      >  1:5999420/1‑137 (MQ=255)

                                                                                             gCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCgcat                                     <  1:19210912/137‑1 (MQ=255)

                                                                                             gCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCgcat                                     <  2:1466767/137‑1 (MQ=255)

                                                                                                 ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCNACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCgcatgcat                                 >  1:18806112/1‑137 (MQ=255)

                                                                                                 ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCCGCgcatgca                                  <  2:9586421/137‑1 (MQ=255)

                                                                                                 ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCgcatgcat                                 >  2:3809417/1‑137 (MQ=255)

                                                                                                 ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCgcatgcat                                 >  2:1205973/1‑137 (MQ=255)

                                                                                                 ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCgcatgcat                                 >  1:5592285/1‑137 (MQ=255)

                                                                                                 ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCgcatgcat                                 >  1:18476798/1‑137 (MQ=255)

                                                                                                 ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCgcatgcat                                 >  2:7693574/1‑137 (MQ=255)

                                                                                                 ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCgcatgcat                                 >  1:751471/1‑137 (MQ=255)

                                                                                                 ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCgcatgcat                                 >  2:350672/1‑137 (MQ=255)

                                                                                                 ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCgcatgcat                                 >  2:3123/1‑137 (MQ=255)

                                                                                                 ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCgcatgcat                                 >  2:14396950/1‑137 (MQ=255)

                                                                                                 ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCgcatgcat                                 >  2:19424677/1‑137 (MQ=255)

                                                                                                 ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCgcatgcat                                 >  2:15565897/1‑137 (MQ=255)

                                                                                                 ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCgcatgcat                                 >  1:16037209/1‑137 (MQ=255)

                                                                                                 ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCgcatgcat                                 >  2:10225014/1‑137 (MQ=255)

                                                                                                 ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCgcatgcat                                 >  2:17223914/1‑137 (MQ=255)

                                                                                                    tttttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCGCATGCATGcc                              >  2:16567620/1‑137 (MQ=255)

                                                                                                    tttttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCGCATGCATGcc                              >  1:1797666/1‑137 (MQ=255)

                                                                                                          aTTCACACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCGCATGCATGCCCAGAAg                        <  1:17753761/137‑1 (MQ=255)

                                                                                                               cACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCGCATGCATGCCCAGAAgctgct                   <  2:623078/137‑1 (MQ=255)

                                                                                                                     gATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGTTGACCAGCCACTACGAGGTGAT‑CCCGCGCATGCATGCCCAGAAGCTGCTGCCgat             <  1:16205895/137‑1 (MQ=255)

                                                                                                                     gATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCGCATGCATGCCCAGAAGCTGCTGCCgat             <  2:19002909/137‑1 (MQ=255)

                                                                                                                     gATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCGCATGCATGCCCAGAAGCTGCTGCCgat             <  1:13164035/137‑1 (MQ=255)

                                                                                                                        aCCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCGCATGCATGCCCAGAAGCTGCTGCCgatgat          >  2:15091149/1‑137 (MQ=255)

                                                                                                                             ttCGATGGCCACCCTGCTGGCCCTGGCTACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGc     >  2:7082900/1‑137 (MQ=255)

                                                                                                                             ttCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGc     >  2:18297561/1‑137 (MQ=255)

                                                                                                                               cGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCGCATGCATGCCCAGAAGCTTCTGCCGATGATCAAGCaa   <  1:17265031/137‑1 (MQ=255)

                                                                                                                               cGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGCaa   >  1:13978041/1‑137 (MQ=255)

                                                                                                                               cGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGCaa   >  2:12553351/1‑137 (MQ=255)

                                                                                                                               cGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGCaa   >  2:5711150/1‑137 (MQ=255)

                                                                                                                               cGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGCaa   >  1:9399724/1‑137 (MQ=255)

                                                                                                                               cGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGCaa   >  1:819241/1‑137 (MQ=255)

GACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGACCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGAT‑CCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGCAAC  >  AE015451_phaM1/1712928‑1713192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`