BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	353,067	A→G	intergenic (+34/+84)	hisF CDS (Imidazole glycerol phosphate synthase subunit HisF) → / ← cbcV CDS (choline _ betaine _ carnitine ABC transporter ‑ ATP binding subunit)	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	353,067	0	A	G	99.8%	1833.6 / NA	478	intergenic (+34/+84)	hisF CDS (Imidazole glycerol phosphate synthase subunit HisF)/cbcV CDS (choline _ betaine _ carnitine ABC transporter ‑ ATP binding subunit)	–/–
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (242/235); minor base T (0/1); total (242/236)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 480 total aligned reads displayed.

TGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCAGGCACGCCCGCTCCC‑ACAGGTTTGCTGAGAT‑CTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGA  >  AE015451_phaM1/352934‑353200

tGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGc                                                                                                                                      >  2:8940132/1‑137 (MQ=255)

tGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGc                                                                                                                                      <  1:12243641/137‑1 (MQ=255)

tGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGc                                                                                                                                      <  2:14327559/137‑1 (MQ=255)

tGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGc                                                                                                                                      >  2:3155287/1‑137 (MQ=255)

 gCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCa                                                                                                                                     >  2:17766459/1‑137 (MQ=255)

   cAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACg                                                                                                                                   >  2:7619436/1‑137 (MQ=255)

   cAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACg                                                                                                                                   >  2:16121963/1‑137 (MQ=255)

   cAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGACCTTCGCGGGCACg                                                                                                                                   <  2:12391244/137‑1 (MQ=255)

    aGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAATGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGc                                                                                                                                  <  2:4623965/137‑1 (MQ=255)

    aGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGc                                                                                                                                  <  2:5866070/137‑1 (MQ=255)

    aGCGCGGTACTGGCGGCCGATATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGgcgcgc                                                                                                                                  <  2:16482828/137‑1 (MQ=255)

         ggTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGcccact                                                                                                                             >  1:4763241/1‑134 (MQ=255)

         ggTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCt                                                                                                                             >  2:13869530/1‑137 (MQ=255)

         ggTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCt                                                                                                                             >  1:18240002/1‑137 (MQ=255)

         ggTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCt                                                                                                                             >  1:18499331/1‑137 (MQ=255)

         ggTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCt                                                                                                                             >  1:16182773/1‑137 (MQ=255)

         ggTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCt                                                                                                                             >  1:8069956/1‑137 (MQ=255)

          gTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGTTTTGCAGCCCTTCGCGGGCACGCCCGCTc                                                                                                                            <  1:7389456/137‑1 (MQ=255)

          gTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTc                                                                                                                            <  1:13535225/137‑1 (MQ=255)

          gTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTc                                                                                                                            >  1:5926965/1‑137 (MQ=255)

          gTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTc                                                                                                                            <  2:1269495/137‑1 (MQ=255)

          gTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTc                                                                                                                            <  1:17733307/137‑1 (MQ=255)

          gTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTc                                                                                                                            <  2:616295/137‑1 (MQ=255)

              tGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTNGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTccccc                                                                                                                        >  1:18023227/1‑135 (MQ=255)

              tGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTccccc                                                                                                                        >  2:2085257/1‑135 (MQ=255)

               ggcggcCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCc‑aca                                                                                                                      <  1:14778916/137‑1 (MQ=255)

               ggcggcCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCc‑aca                                                                                                                      <  2:15386747/137‑1 (MQ=255)

                  ggcCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCCCCC‑ACAAGt                                                                                                                   >  1:792741/1‑137 (MQ=255)

                     cAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTTGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTg                                                                                                                <  2:3696781/137‑1 (MQ=255)

                     cAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCTGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTg                                                                                                                <  2:3028564/137‑1 (MQ=255)

                       gCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCt                                                                                                              <  1:12922830/137‑1 (MQ=255)

                       gCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCt                                                                                                              <  1:5588940/137‑1 (MQ=255)

                       gCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCt                                                                                                              <  1:10746903/137‑1 (MQ=255)

                       gCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCt                                                                                                              <  1:8964551/137‑1 (MQ=255)

                       gCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCt                                                                                                              <  1:9888554/137‑1 (MQ=255)

                       gCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCt                                                                                                              <  1:4050248/137‑1 (MQ=255)

                       gCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCt                                                                                                              <  2:2723483/137‑1 (MQ=255)

                       gCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTCCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCt                                                                                                              <  1:3818390/137‑1 (MQ=255)

                        cATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCTg                                                                                                             >  2:13627324/1‑137 (MQ=255)

                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCTGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCTga                                                                                                            <  2:10090615/137‑1 (MQ=255)

                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGTTGCTTTGCAGCCCTTCGCGGGTACGCCCGCTCCC‑ACAGGTTTGCTga                                                                                                            <  2:19062717/137‑1 (MQ=255)

                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCTga                                                                                                            <  1:6502980/137‑1 (MQ=255)

                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCTga                                                                                                            <  2:3528748/137‑1 (MQ=255)

                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCTga                                                                                                            <  2:19397860/137‑1 (MQ=255)

                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCTga                                                                                                            <  2:1839305/137‑1 (MQ=255)

                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCTga                                                                                                            <  2:5103613/137‑1 (MQ=255)

                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCTga                                                                                                            <  2:17273860/137‑1 (MQ=255)

                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCTga                                                                                                            <  1:1870682/137‑1 (MQ=255)

                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCTga                                                                                                            <  1:2007144/137‑1 (MQ=255)

                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCTga                                                                                                            <  2:8102438/137‑1 (MQ=255)

                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCTga                                                                                                            <  1:1119663/137‑1 (MQ=255)

                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCTga                                                                                                            <  1:8386716/137‑1 (MQ=255)

                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCTga                                                                                                            <  1:9044204/137‑1 (MQ=255)

                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCTga                                                                                                            <  1:9326674/137‑1 (MQ=255)

                             tCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCTGAGAt‑c                                                                                                       <  1:12555229/137‑1 (MQ=255)

                               cACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCTGAGAt‑ctc                                                                                                     <  2:7056233/137‑1 (MQ=255)

                                   ttGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCTGAGAT‑CTCtgtg                                                                                                 <  2:13933497/137‑1 (MQ=255)

                                     ggCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCCCCC‑CAAAGCTTGGTGGGAT‑CTCTGTtgg                                                                                               >  1:969366/1‑137 (MQ=37)

                                     ggCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCACTCCC‑ACAAATTTTCTTAAAt‑ctcctttgg                                                                                               >  1:11426193/1‑131 (MQ=37)

                                                  tGCCGGAGGCAAAGTCCCATCTGGCTTCGCGTGGGATCTTCGTTCTCTGATCCATTAACCGGTGTTGTTTTGAAGCCCTTCTCGGACATGCCCACTCCC‑ACAGGTTTGCTGAGAT‑CTCTGTGGGAGGGGGCGTGccc                                                                                  <  2:11964119/137‑1 (MQ=255)

                                                   gCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCTga                                                                                                            >  1:2697978/1‑111 (MQ=255)

                                                   gCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCTGAGAT‑CTCTGTGGGAGCGGGCGTGCCcg                                                                                 >  2:19239204/1‑137 (MQ=255)

                                                   gCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGAACGCCCGCTCCC‑AAAGGTTTGCTGAGAT‑CTCTGTGGGAGCGGGCGTGCCcg                                                                                 >  2:11078802/1‑137 (MQ=255)

                                                       gAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGGCCGCTCCC‑ACCGGGTTTCTGGGAT‑CTCTGTGGGGGAGGGCGTGCCCGCGaa                                                                             >  1:435805/1‑137 (MQ=38)

                                                       gAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCTGAGAT‑CTCTGTGGGAGCGGGCGTGcccacaaa                                                                             >  1:8740526/1‑132 (MQ=255)

                                                       gAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCTGAGAT‑CTCTGTGGGAGCGGGCGTGCCCGCGaa                                                                             >  2:18835870/1‑137 (MQ=255)

                                                       gAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCACTCCC‑ACAGGTTTGCTGAGAT‑CTCTGTGGGAGCGGGCGTGCCCGCGaa                                                                             >  2:8249013/1‑137 (MQ=255)

                                                       gAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACACCCACTCCC‑CCAAGTTTTCTGAGGT‑CTCTGTGGGGGCGGGCGTGTCCGCaaa                                                                             >  1:11683706/1‑137 (MQ=37)

                                                          gCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCTCCCCC‑CCAGGTTTTTCTAGATCCTCT‑TGGGAGCGGGCGTGCCCTCGAAagg                                                                          >  1:16339378/1‑137 (MQ=25)

                                                          gCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCTGAGGT‑CCCTTTGGGAGCGGGCGTGCCCGCAAAagg                                                                          >  1:6921827/1‑137 (MQ=37)

                                                               ggCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCACTCCC‑ACAAGGTTGTTGGGAG‑TTCTTTGGGGGAGGGTAGGCCCGCAAAAGGAcgcg                                                                     >  1:16725553/1‑137 (MQ=21)

                                                               ggCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACACCCGCCCCC‑ACAAGTTTGTTTCGAT‑CTCTGTGGGAGCGGGCATGCCCCCAAAAGGAcgct                                                                     >  1:15405062/1‑136 (MQ=25)

                                                                 ccTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCACTCCC‑ACAAGTTTGTTGAAAT‑CTCTGTGGGAGCGGGCGTGCCCGCGAAAGGACACGaa                                                                   >  2:560204/1‑137 (MQ=37)

                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGTACGCCCTCCCCC‑CCAAGTTTTTTTGGGT‑CTCTTTGGGGGCGGGTGTGCCCCCGAAAGGACGCGAAACGGaccccact                                                       >  1:5482354/1‑133 (MQ=255)

                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑CCAGAGTTTCTGGGGT‑CTCCGTTGGAGCGGGCGTGCCCACGAAGGGCCCCGAAGCGGCACCaact                                                       >  1:10029688/1‑135 (MQ=25)

                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑CCAAGTTTGCTGAGAT‑CTCTGTGGGGGCGGGCGTGCCCACGAAAGGCCACGAAGCGGCCCCAAtt                                                       >  1:13899180/1‑137 (MQ=38)

                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACCGGTTTGCTGAGAT‑CTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAAtt                                                       >  2:15718584/1‑137 (MQ=255)

                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGTTGGGGT‑CTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAAtt                                                       >  2:14758213/1‑137 (MQ=255)

                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCCCCC‑CCAGGTTTGATGAGAT‑CTCTTTGGGAGCGGTAGTGCCCGCGAAGGGGCGCGAAGCGGCCCCaact                                                       >  1:17043827/1‑135 (MQ=37)

                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGATCCCAACAGG‑TTGATGAGAT‑CTCTGTGGGGGCGGGCGTGTCCGCAAAGGGCCGCGAAGCGGACACAAtt                                                       >  1:15836084/1‑137 (MQ=37)

                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCCCTCCC‑CCCAGGTTGTTGGGGT‑CTCTTTGGGAGCGGGGGTGCCCACGAAAGGCCGCGAAACGGCCCCaaat                                                       >  1:5020112/1‑135 (MQ=25)

                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCACTCCC‑CCCAATTTTCTTGGAT‑CTCTGTTGGAGCGGGCGTGCCCGAGAAAGGCCCCGAAGCGGACCCaact                                                       >  1:13192413/1‑135 (MQ=25)

                                                                                gcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGTTGAGAT‑CTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAACGGCCCCAATTGCt                                                    >  2:6908349/1‑137 (MQ=255)

                                                                                  gggATCGTCGTTCGCTGATCCATTGCCCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGGTTGGTGAGAT‑CTCTGTGGGAGCGGGCATGCCCGCGAAAGGCCGCGAAGCGGCCCAAATTGCTTc                                                  >  2:9582220/1‑137 (MQ=37)

                                                                                  gggATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGTACACCCGCTCCC‑AAAGGTTTGGTGAGGT‑CTCCGTGGGAGTGGGCGTGGCCGCGAAAGGACACGAAGCGGACCCAACTGCttt                                                  >  1:2139213/1‑136 (MQ=25)

                                                                                  gggATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCTGAGAT‑CTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTc                                                  >  2:9379222/1‑137 (MQ=255)

                                                                                          tgtTTGCTGACCCATTGACCGGGGTTGTTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGCTGAGAT‑CTCTGTGGGAGCGGACGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTgg                                          <  2:16939775/136‑1 (MQ=38)

                                                                                                 tGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCACTCCC‑CCAGGTTTGCTTAGAG‑CTCTGTGGGAGCGGGCGTGCCCACGAAAGGCCGCGAAACGGCCCCAACTGCTTCAAACGTGGGTCtttc                                   >  1:3043346/1‑135 (MQ=25)

                                                                                                  gATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGTCACGCCCGCTCCC‑ACAGGTTTGCTGAGAT‑CTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGcc                                  <  2:6686012/137‑1 (MQ=255)

                                                                                                    tCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTGGTTGAGAT‑CTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGa                                <  2:13184350/137‑1 (MQ=255)

                                                                                                                 gctgttTGGCAGCCCTTCGTGGACACACCCGCCCCC‑ACAGGTTTGCTGAGAT‑CTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTa                   <  2:17396567/137‑1 (MQ=38)

                                                                                                                   tgttttGCATCCTTTCGGGGACAACCCCGCTCCC‑ACGGTTTTGTGGAGCC‑CTTTGGGGGAGCGGGCGTACCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTaaa                 <  1:3472999/134‑1 (MQ=21)

                                                                                                                   tgctTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGTTGAGAT‑CTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCCATTGCTTCAACCGTGGTTCTTGCCGAGTAGCGCGTGGTaaa                 >  2:4104376/1‑137 (MQ=255)

                                                                                                                     ctTTGCAGCCCTTCGCGGGCACGCCCGCTCCC‑ACAGGTTTGTTGAGAT‑CTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCACCAATTGCTTCAACCGTGGTTCTTTCCGAGCAGCGCGTGGTAAAGc               >  2:11413937/1‑137 (MQ=255)

                                                                                                                     ctTTGCAGCCCTCTGTGGGCATGCCCGCCCCA‑ACAGTTTGGCTGAGAC‑CTTTGTGGGAGAGGGTGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAAGTGCTTCAACCGTTGTTCTTGCCGAGCAGCGCGTGGTAAAGc               <  1:19124487/137‑1 (MQ=25)

                                                                                                                        tGCAGCCCTTCGCGGGCACGCCCGCTCCC‑AAAGGTTTGCTGAGAT‑CTCTGTGGGAGCGGGCGTGTCCGCGAAGGGCCGCGGAGCGGCCCCAATTGTTTCAACCGTGGTTCTTGCAGAGCAGTGCTTGGTAAAGTTCg            >  2:12643653/1‑137 (MQ=37)

                                                                                                                             cccTTCGCGGGCACGACCGCCTCC‑ACAGGTTTGCTGAGAT‑CTCTGTGGGAGCGGGCGTGCCCGTGAAGGGCCGCGAAGCGGCCCCAATTGTTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTCAAGCTCGGTATc       >  2:6339983/1‑137 (MQ=38)

                                                                                                                                 tCGCGGGCATGCCCGCTCAC‑ACAGGTTTGCTGAGGC‑CTCTGTGGGAGTGGGCGTGCTCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCg   <  2:1038590/137‑1 (MQ=38)

                                                                                                                                 tCGCGGGAACGCCCGCTCCC‑ATAGGTTTGCTGAAAT‑CTTTGTGGGGGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCTTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCg   <  2:4904760/137‑1 (MQ=38)

                                                                                                                                  cgcgGGCACTCCCGCCCCC‑ACGGTTTTGTTGAGAT‑CTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGa  <  1:18892844/137‑1 (MQ=38)

TGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCAGGCACGCCCGCTCCC‑ACAGGTTTGCTGAGAT‑CTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGA  >  AE015451_phaM1/352934‑353200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`