Predicted mutation
evidence seq id position mutation annotation gene description
RA pS4413_qbsFH 108 G→A intergenic (–/‑1845) – / → aadA CDS –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*pS4413_qbsFH1080GA99.8% 71579.6 / NA 19786intergenic (–/‑1845)–/aadA CDS–/–
Reads supporting (aligned to +/- strand):  ref base G (7/5);  major base A (13398/6337);  minor base C (34/5);  total (13439/6347)
Fisher's exact test for biased strand distribution p-value = 9.19e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 19788 total aligned reads displayed.

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCTGGGACTGGATAAAGTGCTGG  >  pS4413_qbsFH/1‑241
                                                                                                                                    |                                                                                                                                     
gagcctttcgttttatttgatgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATAcc                                                                                                                                   >  2:9606191‑M1/26‑137 (MQ=255)
   cctttcgttttatttgatgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCagg                                                                                                                                <  1:11008163‑M1/115‑1 (MQ=255)
   cctttcgttttatttgatgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCagg                                                                                                                                <  1:11358500‑M1/115‑1 (MQ=255)
   cctttcgttttatttgatgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCagg                                                                                                                                >  2:5564896‑M1/23‑137 (MQ=255)
   cctttcgttttatttgatgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCagg                                                                                                                                <  2:11192276‑M1/115‑1 (MQ=255)
   cctttcgttttatttgatgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCagg                                                                                                                                <  1:913197‑M1/115‑1 (MQ=255)
    ctttcgttttatttgatgcctTTCATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCagga                                                                                                                               <  1:2306539‑M1/116‑1 (MQ=255)
    ctttcgttttatttgatgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCagga                                                                                                                               <  1:9794786‑M1/116‑1 (MQ=255)
           tttatttgatgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGaaaaa                                                                                                                        <  2:6311889‑M1/123‑1 (MQ=255)
             tatttgatgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACa                                                                                                                      >  2:15933782‑M1/13‑137 (MQ=255)
             tatttgatgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACa                                                                                                                      >  1:15166419‑M1/13‑137 (MQ=255)
              atttgatgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACat                                                                                                                     <  1:946746‑M1/126‑1 (MQ=255)
                ttgatgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACatat                                                                                                                   <  1:5450103‑M1/128‑1 (MQ=255)
                   atgcctTTAATTAATTGTTGACAGTTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGNTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATAtgtg                                                                                                                >  1:16822633‑M1/7‑137 (MQ=255)
                   atgcctTTAATTAATTGTTGACAATTAATCATCGGCATGGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATAtgtg                                                                                                                >  1:10000175‑M1/7‑137 (MQ=255)
                   atgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATAtgtg                                                                                                                >  2:7423433‑M1/7‑137 (MQ=255)
                   atgcctTTAAGTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATAtgtg                                                                                                                >  2:1994970‑M1/7‑137 (MQ=255)
                    tgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGc                                                                                                               >  2:5190250‑M1/6‑137 (MQ=255)
                    tgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGc                                                                                                               >  1:781513‑M1/6‑137 (MQ=255)
                    tgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGc                                                                                                               >  1:266999‑M1/6‑137 (MQ=255)
                    tgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAAGACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGc                                                                                                               >  2:8114181‑M1/6‑137 (MQ=255)
                      cctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGccc                                                                                                             <  1:18194416‑M1/134‑1 (MQ=255)
                       ctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCt                                                                                                            <  1:137442‑M1/135‑1 (MQ=255)
                        tTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCtt                                                                                                           >  1:16517575‑M1/2‑137 (MQ=255)
                         ttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTg                                                                                                          <  2:13734717/137‑1 (MQ=255)
                                   gttgACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATg                                                                                                >  1:3333729/1‑137 (MQ=255)
                                    ttgACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGc                                                                                               <  1:5058340/137‑1 (MQ=255)
                                    ttgACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGc                                                                                               >  1:12645905/1‑137 (MQ=255)
                                    ttgACAATTAAGCAGCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGc                                                                                               <  2:10858432/137‑1 (MQ=255)
                                     tgACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAACCCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTAAATGCa                                                                                              >  1:19511485/1‑137 (MQ=255)
                                     tgACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCa                                                                                              >  2:17614947/1‑137 (MQ=255)
                                     tgACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCa                                                                                              >  2:2343890/1‑137 (MQ=255)
                                        cAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCacca                                                                                           <  2:17217443/137‑1 (MQ=255)
                                        cAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCacca                                                                                           <  1:4342272/137‑1 (MQ=255)
                                         aaTTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCaccaa                                                                                          >  2:14479147/1‑137 (MQ=255)
                                          aTTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCaccaaa                                                                                         <  1:7474504/137‑1 (MQ=255)
                                          aTTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCaccaaa                                                                                         <  1:8117187/137‑1 (MQ=255)
                                                   cggcatagtatatcggcatagtataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAAcccc                                                                                <  1:188778/137‑1 (MQ=255)
                                                      catagtatatcggcatagtataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAAc                                                                             <  2:10159240/137‑1 (MQ=255)
                                                            atatcggcatagtataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGc                                                                       >  2:7027747/1‑137 (MQ=255)
                                                               tcggcatagtataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTTGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCACAACAGTGGCATg                                                                    >  2:16263003/1‑137 (MQ=255)
                                                               tcggcatagtataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATg                                                                    <  2:8493924/137‑1 (MQ=255)
                                                               tcggcatagtataAGACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATg                                                                    <  1:8477974/137‑1 (MQ=255)
                                                                cggcatagtataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGa                                                                   >  1:3981439/1‑137 (MQ=255)
                                                                   catagtataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCACACCAAACCCCAACAGTGGCATGACGc                                                                >  2:3395095/1‑137 (MQ=255)
                                                                   catagtataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGc                                                                >  1:3669457/1‑137 (MQ=255)
                                                                   catagtataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTAAATGCACCAAACCAAACCCCAACAGTGGCATGACGc                                                                >  2:16595779/1‑137 (MQ=255)
                                                                      agtataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAAGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCaa                                                             >  2:4873713/1‑137 (MQ=255)
                                                                        tataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACGAAGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGCCGCCAAAc                                                           >  2:15548789/1‑137 (MQ=255)
                                                                        tataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAAc                                                           >  2:3042786/1‑137 (MQ=255)
                                                                        tataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAAc                                                           >  1:14778120/1‑137 (MQ=255)
                                                                        tataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAAc                                                           >  2:12249121/1‑137 (MQ=255)
                                                                        tataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAAc                                                           >  1:10675893/1‑137 (MQ=255)
                                                                                gACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTc                                                   >  1:2968047/1‑137 (MQ=255)
                                                                                gACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTAAATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTc                                                   >  2:4137214/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGACTTCGAGCTCGATACCAGGAGAAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                              >  1:7169729/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                              >  2:16890307/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                              >  1:1582392/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                              >  2:9239873/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                              >  2:15251180/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                              >  2:14914877/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                              >  2:13370340/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                              >  1:6118564/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                              >  1:6796023/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                              >  2:13033616/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                              >  2:5930354/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                              >  1:8802468/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                              >  1:18563055/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                              >  1:14118663/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                              >  1:19207220/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCAAAACTGGACTTCGCCGa                                              >  1:17168544/1‑137 (MQ=255)
                                                                                        gAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAAt                                           >  2:18827551/1‑137 (MQ=255)
                                                                                         aGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATc                                          <  2:19214409/137‑1 (MQ=255)
                                                                                              cTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAg                                     <  1:14415876/137‑1 (MQ=255)
                                                                                              cTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCAATACCAGGAGGAAAAACATATGTACCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAg                                     <  1:13338341/137‑1 (MQ=255)
                                                                                               tAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGt                                    >  2:1265639/1‑137 (MQ=255)
                                                                                                  aCCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTAc                                 >  1:12295647/1‑137 (MQ=255)
                                                                                                   ccccTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACg                                >  1:17501050/1‑137 (MQ=255)
                                                                                                   ccccTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACg                                >  2:10495216/1‑137 (MQ=255)
                                                                                                   ccccTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACg                                >  1:17860994/1‑137 (MQ=255)
                                                                                                    cccTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACgg                               <  2:3743852/137‑1 (MQ=255)
                                                                                                    cccTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACgg                               <  1:6457915/137‑1 (MQ=255)
                                                                                                    cccTAGGCCGCGGCCGCGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACgg                               <  1:4709338/137‑1 (MQ=255)
                                                                                                         ggccgcggccgcGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGAtt                          >  2:18406960/1‑137 (MQ=255)
                                                                                                         ggccgcggccgcGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGAtt                          <  2:7796852/137‑1 (MQ=255)
                                                                                                         ggccgcggccgcGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGAtt                          <  1:16176159/137‑1 (MQ=255)
                                                                                                         ggccgcggccgcGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGAtt                          <  2:11914999/137‑1 (MQ=255)
                                                                                                         ggccgcggccgcGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGAtt                          >  2:8842645/1‑137 (MQ=255)
                                                                                                         ggccgcggccgcGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGACGAAATCATGAGTTACGGCGAtt                          >  2:18030442/1‑137 (MQ=255)
                                                                                                         ggccgcggccgcGCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACGTCGCCGAAATCATGAGGTACGGCGAtt                          <  2:14113574/137‑1 (MQ=255)
                                                                                                             gcggccacgCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCt                      <  2:2706590/137‑1 (MQ=255)
                                                                                                              cggccgcgCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCTg                     <  2:11592536/137‑1 (MQ=255)
                                                                                                                 ccgcgCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCTGGGa                  >  2:6668843/1‑137 (MQ=255)
                                                                                                                 ccgcgCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCTGGGa                  >  1:11647396/1‑137 (MQ=255)
                                                                                                                 ccgcgCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCTGGGa                  >  1:11953699/1‑137 (MQ=255)
                                                                                                                 ccgcgCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCTGGGa                  >  1:13002294/1‑137 (MQ=255)
                                                                                                                 ccgcgCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCTGGGa                  >  1:5810245/1‑137 (MQ=255)
                                                                                                                 ccgcgCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCTGGGa                  >  1:15481381/1‑137 (MQ=255)
                                                                                                                 ccgcgCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCTGGGa                  >  1:10307997/1‑137 (MQ=255)
                                                                                                                 ccgcgCGAATTCGAGCTCGATACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCACAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGCGTTCCGGCGATTACCTGGGa                  >  2:4530264/1‑137 (MQ=255)
                                                                                                                                    |                                                                                                                                     
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCTGGGACTGGATAAAGTGCTGG  >  pS4413_qbsFH/1‑241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑