Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451_phaM1 539,294 A→C T558P (ACC→CCC)  rpoB CDS (DNA‑directed RNA polymerase subunit beta) →

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM1539,2940AC100.0% 2937.6 / NA 721T558P (ACC→CCC) rpoB CDS (DNA‑directed RNA polymerase subunit beta)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (339/382);  total (339/382)

Only 100 of 744 total aligned reads displayed.

CAGCCAGCTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGACCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTACCGCGTGGTGAAGGAAG  >  AE015451_phaM1/539161‑539426
                                                                                                                                     |                                                                                                                                    
cagccagcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCTTAGATTACACACAAGCGCCGCGTCTCCGCACTCTGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGcccc                                                                                                                                   <  2:19181246/137‑1 (MQ=255)
cagccagcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGcccc                                                                                                                                   <  2:8144011/137‑1 (MQ=255)
cagccagcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGcccc                                                                                                                                   >  2:6578325/1‑137 (MQ=255)
cagccagcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGcccc                                                                                                                                   <  2:3723566/137‑1 (MQ=255)
cagccagcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGcccc                                                                                                                                   <  2:2789719/137‑1 (MQ=255)
cagccagcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGcccc                                                                                                                                   <  2:217993/137‑1 (MQ=255)
cagccagcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGcccc                                                                                                                                   <  2:17287487/137‑1 (MQ=255)
cagccagcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGcccc                                                                                                                                   <  2:11733907/137‑1 (MQ=255)
  gccagcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCAc                                                                                                                                 >  2:15294045/1‑137 (MQ=255)
    cagcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCTGAGATTACCCACAAACGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCTCCCCACTa                                                                                                                               <  2:13558931/137‑1 (MQ=255)
    cagcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGNACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTa                                                                                                                               <  1:16795937/137‑1 (MQ=255)
    cagcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTa                                                                                                                               <  2:5652905/137‑1 (MQ=255)
    cagcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTa                                                                                                                               <  2:5397522/137‑1 (MQ=255)
    cagcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTa                                                                                                                               <  1:1974974/137‑1 (MQ=255)
     agcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTAt                                                                                                                              >  2:9481003/1‑137 (MQ=255)
      gcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATg                                                                                                                             <  2:18909289/137‑1 (MQ=255)
        tGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGc                                                                                                                           <  1:17425294/137‑1 (MQ=255)
          tCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCg                                                                                                                         >  1:8656877/1‑137 (MQ=255)
          tCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCg                                                                                                                         >  1:18963274/1‑137 (MQ=255)
               gTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCgtgtgt                                                                                                                    <  2:17955685/137‑1 (MQ=255)
               gTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCgtgtgt                                                                                                                    <  1:9142669/137‑1 (MQ=255)
               gTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCgtgtgt                                                                                                                    <  1:15890364/137‑1 (MQ=255)
                     ggACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGa                                                                                                              >  1:17934164/1‑137 (MQ=255)
                     ggACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGa                                                                                                              >  1:11277917/1‑137 (MQ=255)
                      gACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGAt                                                                                                             <  2:14977198/137‑1 (MQ=255)
                      gACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGAt                                                                                                             <  1:14638117/137‑1 (MQ=255)
                      gACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGAt                                                                                                             <  2:7003080/137‑1 (MQ=255)
                       aCCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATc                                                                                                            >  1:18666816/1‑137 (MQ=255)
                        ccAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCg                                                                                                           >  1:12165158/1‑137 (MQ=255)
                                    tctctcGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAg                                                                                               >  2:8547450/1‑137 (MQ=255)
                                    tctctcGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAg                                                                                               >  1:10762307/1‑137 (MQ=255)
                                         cGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCg                                                                                          >  1:5885152/1‑137 (MQ=255)
                                         cGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCg                                                                                          >  1:10047632/1‑137 (MQ=255)
                                         cGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCg                                                                                          >  1:2592500/1‑137 (MQ=255)
                                              aTTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACAt                                                                                     >  1:7705550/1‑137 (MQ=255)
                                              aTTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACAt                                                                                     >  2:18656233/1‑137 (MQ=255)
                                               ttACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATc                                                                                    <  1:15240647/137‑1 (MQ=255)
                                               ttACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATc                                                                                    >  1:7328677/1‑137 (MQ=255)
                                                tACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCTGAACATCg                                                                                   <  1:7049427/137‑1 (MQ=255)
                                                 aCCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCgg                                                                                  <  2:1382511/137‑1 (MQ=255)
                                                   ccACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTc                                                                                >  2:10779359/1‑137 (MQ=255)
                                                   ccACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTc                                                                                >  2:16274327/1‑137 (MQ=255)
                                                   ccACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGATc                                                                                >  2:12718300/1‑137 (MQ=255)
                                                    cacaAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCt                                                                               <  2:14681470/137‑1 (MQ=255)
                                                     acaAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTg                                                                              >  1:3699342/1‑137 (MQ=255)
                                                         gcgcCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCa                                                                          >  1:9526280/1‑137 (MQ=255)
                                                           gccgcGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAAc                                                                        <  2:8941511/137‑1 (MQ=255)
                                                           gccgcGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAAc                                                                        >  2:12100326/1‑137 (MQ=255)
                                                             cgcgTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGcccc                                                                                                                                   >  1:13849444/1‑76 (MQ=255)
                                                               cgTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTccc                                                                    <  1:14882606/137‑1 (MQ=255)
                                                               cgTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTccc                                                                    >  1:6194977/1‑137 (MQ=255)
                                                               cgTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTccc                                                                    <  1:2165939/137‑1 (MQ=255)
                                                               cgTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTccc                                                                    >  2:7733006/1‑137 (MQ=255)
                                                               cgTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTccc                                                                    <  1:9308610/137‑1 (MQ=255)
                                                               cgTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTccc                                                                    <  1:5056961/137‑1 (MQ=255)
                                                               cgTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTccc                                                                    <  2:7352700/137‑1 (MQ=255)
                                                               cgTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTccc                                                                    <  1:33293/137‑1 (MQ=255)
                                                               cgTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTccc                                                                    <  2:11481854/137‑1 (MQ=255)
                                                               cgTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTccc                                                                    >  1:17142503/1‑137 (MQ=255)
                                                               cgTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCACGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTccc                                                                    <  1:10467789/137‑1 (MQ=255)
                                                                  ctcCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTgg                                                                 >  2:2956547/1‑137 (MQ=255)
                                                                    ccGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTggcg                                                               <  1:6449522/137‑1 (MQ=255)
                                                                         cTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATTGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTa                                                          <  1:3179808/137‑1 (MQ=255)
                                                                         cTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTa                                                          <  1:16544780/137‑1 (MQ=255)
                                                                           cGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATg                                                        <  1:555691/137‑1 (MQ=255)
                                                                             gCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGcc                                                      >  2:10983016/1‑137 (MQ=255)
                                                                              cccTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGccc                                                     <  2:6086885/137‑1 (MQ=255)
                                                                              cccTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATAccc                                                     <  1:11813517/137‑1 (MQ=255)
                                                                               ccTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGGAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCg                                                    >  1:88363/1‑137 (MQ=255)
                                                                               ccTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCCCTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCACCTCCCTGGCGGCCTATGCCCg                                                    >  1:1827440/1‑137 (MQ=255)
                                                                               ccTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCg                                                    >  2:18135646/1‑137 (MQ=255)
                                                                               ccTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCg                                                    >  1:3944654/1‑137 (MQ=255)
                                                                               ccTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCg                                                    >  1:8127653/1‑137 (MQ=255)
                                                                               ccTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCg                                                    >  1:16149861/1‑137 (MQ=255)
                                                                                cTGGCGGGCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGGGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGc                                                   <  1:2856899/137‑1 (MQ=255)
                                                                                 tGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCa                                                  <  1:15707765/137‑1 (MQ=255)
                                                                                        cTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCaccaacca                                           <  1:18502199/137‑1 (MQ=255)
                                                                                          gACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGt                                         <  2:18348945/137‑1 (MQ=255)
                                                                                              cGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACgg                                     >  2:14240188/1‑137 (MQ=255)
                                                                                                   gCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTcc                                >  2:10310319/1‑137 (MQ=255)
                                                                                                   gCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTcc                                <  1:4167228/137‑1 (MQ=255)
                                                                                                   gCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTcc                                >  2:4133482/1‑137 (MQ=255)
                                                                                                   gCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTcc                                <  2:310188/137‑1 (MQ=255)
                                                                                                   gCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTcc                                <  1:13462731/137‑1 (MQ=255)
                                                                                                     gTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTg                              <  2:4531195/137‑1 (MQ=255)
                                                                                                        ccGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGaa                           <  2:12956090/137‑1 (MQ=255)
                                                                                                         cGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGaaa                          >  1:6664567/1‑137 (MQ=255)
                                                                                                          ggCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAg                         >  1:17052140/1‑137 (MQ=255)
                                                                                                           gCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGc                        >  2:4899127/1‑137 (MQ=255)
                                                                                                                 aaGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTAc                  <  1:17736421/137‑1 (MQ=255)
                                                                                                                 aaGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTAc                  <  1:5299238/137‑1 (MQ=255)
                                                                                                                  aGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGATACCCCTGAAGGTCCGAACATCGGTCTGAGCAACTCCCTGGCGGCCTATGCCTGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTAcc                 <  1:15485094/137‑1 (MQ=255)
                                                                                                                  aGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTAcc                 <  1:12714174/137‑1 (MQ=255)
                                                                                                                  aGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTAcc                 <  1:14202325/137‑1 (MQ=255)
                                                                                                                  aGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTAcc                 <  2:15793658/137‑1 (MQ=255)
                                                                                                                         gACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTACCGCgtggt          >  2:16713542/1‑137 (MQ=255)
                                                                                                                         gACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTACCGCgtggt          >  2:11172274/1‑137 (MQ=255)
                                                                                                                           cgcacacCCGCCCCACCATAGCTACGCGTGCCCGATCGACACCCCTGAAGGTCCGGACATCGTTCGGATCAACTCTCTGGTGGCAGATGCTCGCACCAGCCGGTCTGGATTCCGGGAAAGCCCGTACCGCGTGGTga        <  2:13245389/134‑1 (MQ=255)
                                                                                                                           cGTACACCCGCCCCGCTATGGTCGTGTGTGCTCGATCTAGACCCCTTAAGGTCCGAGCATCGGTCTGATCGGCTCCCTGGCGGCCTATGCCCGCACCAACCAGTACTGCTTTCTGGGAGGCCCGTACCGCgtgggta        <  2:2449392/137‑4 (MQ=255)
                                                                                                                           cGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTACCGCGTGGTga        <  1:9880088/137‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                    
CAGCCAGCTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGACCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTACCGCGTGGTGAAGGAAG  >  AE015451_phaM1/539161‑539426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑