Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_012967	70,867	T→C	D92G (GAC→GGC)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012967	70,867	0	T	C	100.0%	162.0 / NA	44	D92G (GAC→GGC)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/20);  total (24/20)

TATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGTCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCG  >  NC_012967/70790‑70944                                                                              |                                                                              tatCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGGGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGtt                                                                           >  1:604790/1‑82 (MQ=255)  atCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGGGTGGAATTGCAGCAACGGATTGTTTACCATAGCCAGGCCGTTg                                                                          >  1:1246232/1‑82 (MQ=255)   tCGAGACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGa                                                                         <  1:3215564/82‑1 (MQ=255)    cGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAGTTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGAt                                                                        >  1:178047/1‑82 (MQ=255)    cGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGAt                                                                        <  1:749297/82‑1 (MQ=255)    cGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGGTGAGCATGGTCAGGACGTTGAt                                                                        >  1:1810451/1‑82 (MQ=255)     gagaCTGGCCCACGGCAGCGCCGCGGTGAACTGGGTGTGCAAATGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATc                                                                       <  1:552947/79‑1 (MQ=255)     gATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATc                                                                       <  1:80676/82‑1 (MQ=255)      aTACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATcc                                                                      >  1:2017595/1‑82 (MQ=255)       tACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCca                                                                     >  1:3451418/1‑82 (MQ=255)        aCTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCcac                                                                    >  1:2505998/1‑82 (MQ=255)        aCTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCcac                                                                    >  1:3437147/1‑82 (MQ=255)        aCTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCcac                                                                    <  1:388051/82‑1 (MQ=255)          tGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACAt                                                                  >  1:3081564/1‑82 (MQ=255)          tGTCCCACGGCAGCGCCGCGTTGAACTGGGGGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGCTCCACAt                                                                  >  1:119251/1‑82 (MQ=255)           gTCCCACGTCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACAtt                                                                 <  1:900099/82‑1 (MQ=255)           gTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACAtt                                                                 <  1:1136044/82‑1 (MQ=255)             cccACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACAtttt                                                               <  1:1468281/82‑1 (MQ=255)              ccACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTg                                                              <  1:2096340/82‑1 (MQ=255)              ccACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTg                                                              >  1:3121865/1‑82 (MQ=255)                aCGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGc                                                            <  1:654775/82‑1 (MQ=255)                aCGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGc                                                            >  1:1053005/1‑82 (MQ=255)                  ggCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGATTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCg                                                          <  1:3677966/82‑1 (MQ=255)                   gCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCgg                                                         <  1:1051698/82‑1 (MQ=255)                    ccgcgcCGCGGTGAACTGGGTGTGGAATTGCAGCAACGGTTGGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCggg                                                        <  1:1985294/80‑1 (MQ=255)                      gcgcCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGga                                                      >  1:1031154/1‑82 (MQ=255)                      gcgcCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGga                                                      <  1:1466658/82‑1 (MQ=255)                      gcgcCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGga                                                      <  1:2071590/82‑1 (MQ=255)                             gTTGAACTGGGTGTGGATTTGCAGCAACGTTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGgtg                                               <  1:3699725/82‑1 (MQ=255)                                  aCTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAg                                          <  1:1089831/82‑1 (MQ=255)                                   cTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGc                                         >  1:2426985/1‑82 (MQ=255)                                     gggTGTGGAATTGCAGCACCGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGAca                                       >  1:2223902/1‑82 (MQ=255)                                      ggTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCcac                                      >  1:406573/1‑82 (MQ=255)                                      ggTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCcac                                      >  1:3291674/1‑82 (MQ=255)                                      ggTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCcac                                      >  1:2067125/1‑82 (MQ=255)                                        tggggAATTGCAGCAACGTTTGGTGGAGCAGGGTCAGGCCGTTGATCCACATTTGGGCC‑GGGAGAAGGTGTGCAGCCAcacc                                   <  1:3023323/79‑1 (MQ=255)                                          tgGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGTCCGGGGAGAAGGTGTGCAGCCAcacca                                  >  1:3657472/1‑82 (MQ=255)                                          tgGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCAcacca                                  >  1:3464771/1‑82 (MQ=255)                                           ggAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCAcaccac                                 >  1:2008459/1‑82 (MQ=255)                                              aTTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCaccag                              <  1:2574192/82‑1 (MQ=255)                                              aTTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCaccag                              >  1:679631/1‑82 (MQ=255)                                                tGCAGCAATGGTTTTTTGGGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCaccagac                            <  1:2991856/82‑1 (MQ=255)                                                tGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCaccagac                            <  1:3559960/82‑1 (MQ=255)                                                tGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCaccagac                            <  1:430030/82‑1 (MQ=255)                                                tGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTGGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCaccagac                            <  1:3559961/82‑1 (MQ=255)                                                   agcaACGGTTTGTTGAGCATTGTCATGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCGCACCaccaggccag                         <  1:2271197/82‑1 (MQ=255)                                                         ggTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAAc                   >  1:1663412/1‑82 (MQ=255)                                                              gttgAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGAtca              >  1:1737577/1‑81 (MQ=255)                                                              gttaAGCATGGTCAGACCGTTTATCCACATTTTGGCAGGGGAGAAGTTGTGCAGCCACACCACCAGACCGGCGCAACGAtcg              <  1:1029147/82‑1 (MQ=255)                                                                 gAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGAtcgtcg           >  1:3130776/1‑82 (MQ=255)                                                                   gCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTa         >  1:673856/1‑82 (MQ=255)                                                                   gCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTa         >  1:1325305/1‑82 (MQ=255)                                                                          cAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATtcgcg  >  1:459528/1‑82 (MQ=255)                                                                              |                                                                              TATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGTCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCG  >  NC_012967/70790‑70944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑