Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_012967	2,847,052	A→G	V10A (GTT→GCT)	recD ←	exodeoxyribonuclease V subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012967	2,847,052	0	A	G	100.0%	299.6 / NA	72	V10A (GTT→GCT)	recD	exodeoxyribonuclease V subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/41);  total (31/41)

GCCGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAACTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCT  >  NC_012967/2846965‑2847137                                                                                        |                                                                                      gCCGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCt                                                                                     <  1:4110249/90‑1 (MQ=255)   cgcgAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTc                                                                                   <  1:3596547/90‑1 (MQ=255)    gcgAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTcc                                                                                  >  1:2133549/1‑90 (MQ=255)    gcgAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTcc                                                                                  >  1:1965106/1‑90 (MQ=255)     cgAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCa                                                                                 <  1:1160994/90‑1 (MQ=255)     cgAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCa                                                                                 >  1:2979375/1‑90 (MQ=255)     cgAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCa                                                                                 <  1:2651922/90‑1 (MQ=255)      gAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAg                                                                                >  1:1252103/1‑90 (MQ=255)       aGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGt                                                                               <  1:4297859/90‑1 (MQ=255)       aGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGt                                                                               >  1:2994641/1‑90 (MQ=255)         ggTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTaa                                                                             <  1:895399/90‑1 (MQ=255)         ggTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTaa                                                                             >  1:3094049/1‑90 (MQ=255)          gTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAAt                                                                            <  1:1686048/90‑1 (MQ=255)             aCGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGc                                                                         >  1:1850044/1‑90 (MQ=255)              cGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCt                                                                        <  1:1867300/90‑1 (MQ=255)               ggCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGcac                                                                       >  1:2407252/1‑88 (MQ=255)               ggCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCtt                                                                       >  1:2231695/1‑90 (MQ=255)                 cAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCtttt                                                                     >  1:1720413/1‑90 (MQ=255)                     aTGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCaa                                                                 >  1:604702/1‑90 (MQ=255)                     aTGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCaa                                                                 <  1:3210664/90‑1 (MQ=255)                         tCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTc                                                             <  1:1429467/90‑1 (MQ=255)                          cATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCa                                                            <  1:30264/90‑1 (MQ=255)                          cATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCa                                                            <  1:2947072/90‑1 (MQ=255)                           aTCCCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCAt                                                           <  1:1157981/90‑1 (MQ=255)                            tctcCCGCCACGGTCAGGGCAAATTTCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCAtt                                                          <  1:3224916/90‑1 (MQ=255)                            tctcCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCAtt                                                          >  1:2308830/1‑90 (MQ=255)                              tcCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTAc                                                        <  1:701415/90‑1 (MQ=255)                                 cGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGcc                                                     <  1:5687/90‑1 (MQ=255)                                   ccACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGcctc                                                   <  1:3783376/90‑1 (MQ=255)                                    cACGGTCAGGGCAAATTGCCCATCCAGCGGGCGTAGCTGTTTGTGCTCCCCAGCTTCCCGTACTTGCTTTTGCAATTTCATTACGcctcc                                                  >  1:368622/1‑90 (MQ=255)                                    cACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGcctcc                                                  >  1:3858354/1‑90 (MQ=255)                                       ggTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCtcctcc                                               <  1:2294801/90‑1 (MQ=255)                                            gggCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGt                                          <  1:545525/90‑1 (MQ=255)                                             ggCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTc                                         >  1:202680/1‑90 (MQ=255)                                               cAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCAt                                       <  1:361732/90‑1 (MQ=255)                                                 aaTTGCACATCCAGCGGGCGTGGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATAc                                     <  1:2661004/90‑1 (MQ=255)                                                 aaTTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATAc                                     >  1:4351008/1‑90 (MQ=255)                                                 aaTTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATAc                                     >  1:2177552/1‑90 (MQ=255)                                                   ttGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCTAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCg                                   <  1:2434780/90‑1 (MQ=255)                                                   ttGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCg                                   >  1:3941032/1‑90 (MQ=255)                                                    tGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCgg                                  <  1:3357784/90‑1 (MQ=255)                                                      cacaTCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCa                                <  1:169713/90‑1 (MQ=255)                                                       acaTCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCaa                               >  1:1258663/1‑90 (MQ=255)                                                        caTCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCaaa                              <  1:2407537/90‑1 (MQ=255)                                                        caTCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGACagt                              >  1:136677/1‑88 (MQ=255)                                                         aTCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAAc                             >  1:9830/1‑90 (MQ=255)                                                              gCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACAtctc                        >  1:3257565/1‑90 (MQ=255)                                                              gCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACAtctc                        <  1:402218/90‑1 (MQ=255)                                                              gCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTACAGGGTCATACCGGCAAACAtctc                        >  1:1716632/1‑90 (MQ=255)                                                                     tAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTcatccatc                 >  1:254081/1‑90 (MQ=255)                                                                      aGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCa                >  1:1608351/1‑90 (MQ=255)                                                                       gCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAg               >  1:4304422/1‑90 (MQ=255)                                                                       gCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAg               >  1:4304410/1‑90 (MQ=255)                                                                        cTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAgg              <  1:3585702/90‑1 (MQ=255)                                                                        cTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAgg              <  1:3741251/90‑1 (MQ=255)                                                                          gTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGtc            <  1:1170720/90‑1 (MQ=255)                                                                             tgtgCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGtcaat         <  1:1426365/90‑1 (MQ=255)                                                                             tgtgCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGtcaat         <  1:2190034/90‑1 (MQ=255)                                                                             tgtgCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGtcaat         <  1:3833147/90‑1 (MQ=255)                                                                              gtgCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGtcaatc        <  1:3971193/90‑1 (MQ=255)                                                                              gtgCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGtcaatc        >  1:1298590/1‑90 (MQ=255)                                                                              gtgCTCCACAGCTTCCAGTAATTGCTTATGCAATTTCAGTACGCCTCCTCCAGGGTCATACCGGCAAACATCCCATCCATCAGGtcaatc        <  1:510256/90‑1 (MQ=255)                                                                                  tCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAAcc    >  1:2968869/1‑90 (MQ=255)                                                                                  tCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAAcc    <  1:4427483/90‑1 (MQ=255)                                                                                   ccACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAAtcc   <  1:481268/90‑1 (MQ=255)                                                                                   ccACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAAccc   <  1:2111317/90‑1 (MQ=255)                                                                                   ccACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAAccc   <  1:3857558/90‑1 (MQ=255)                                                                                   ccACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCAGCAGGTCAATCAAccc   <  1:3009770/90‑1 (MQ=255)                                                                                    cacaGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCt  >  1:4090233/1‑90 (MQ=255)                                                                                    cacaGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCt  <  1:1439590/90‑1 (MQ=255)                                                                                    cacaGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCt  >  1:2191909/1‑90 (MQ=255)                                                                                    cacaGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCCCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCt  <  1:2743571/90‑1 (MQ=255)                                                                                        |                                                                                      GCCGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAACTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCT  >  NC_012967/2846965‑2847137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑