Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_012967	3,744,568	A→C	L287R (CTA→CGA)	waaG ←	glycosyltransferase family 4 protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012967	3,744,568	0	A	C	100.0%	325.6 / NA	76	L287R (CTA→CGA)	waaG	glycosyltransferase family 4 protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (35/41);  total (35/41)

CGCCGCAATTGGCGTCGACAATATAATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTAGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAATTCCGAGACATCGTTGCGTCCCGAG  >  NC_012967/3744482‑3744654                                                                                       |                                                                                       cgccgcAATTGGCGTCGACAATATAATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGca                                                                                     <  1:3979948/90‑1 (MQ=255) cgccgcAATTGGCGTCGACAATATAATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGca                                                                                     <  1:77639/90‑1 (MQ=255)  gccgcAATTGGCGTCGACAATATAATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGcac                                                                                    >  1:705111/1‑90 (MQ=255)  gccgcAATTGGCGTCGACAATATAATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGcac                                                                                    <  1:3421697/90‑1 (MQ=255)     gcAATTGGCGTCGACAATATAATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAAt                                                                                 >  1:470380/1‑90 (MQ=255)      cAATTGGCGTCGACAATATAATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAAtt                                                                                <  1:3262418/90‑1 (MQ=255)      cAATTGGCGTCGACAATATAATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAAtt                                                                                >  1:891761/1‑90 (MQ=255)       aaTTGGCGTCGACAATATAATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTc                                                                               >  1:2550622/1‑90 (MQ=255)        aTTGGCGTCGACAATATAATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTcc                                                                              >  1:972491/1‑90 (MQ=255)         ttGGCGTCGACAATATAATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTccc                                                                             >  1:1657919/1‑90 (MQ=255)            gCGTCGACAATATAATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCgct                                                                          <  1:2527562/90‑1 (MQ=255)             cGTCGACAATATAATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCgctg                                                                         <  1:3308827/90‑1 (MQ=255)              gTCGACAATATAATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCgctgc                                                                        <  1:2720713/90‑1 (MQ=255)              gTCGACAATATAATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCgctgc                                                                        >  1:4046026/1‑90 (MQ=255)               tCGACAATATAATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCgctgct                                                                       >  1:1177583/1‑90 (MQ=255)                 gACAATATAATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTc                                                                     >  1:4366504/1‑90 (MQ=255)                 gACAATATAATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTc                                                                     <  1:3263414/90‑1 (MQ=255)                   cAATATAATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCt                                                                   <  1:4105783/90‑1 (MQ=255)                    aaTATAATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTg                                                                  <  1:2524358/90‑1 (MQ=255)                     atatAATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTgg                                                                 >  1:3696061/1‑90 (MQ=255)                     atatAATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTgg                                                                 >  1:1823949/1‑90 (MQ=255)                      tataATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGt                                                                <  1:955780/90‑1 (MQ=255)                      tataATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGt                                                                <  1:1355145/90‑1 (MQ=255)                      tataATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGt                                                                <  1:2817292/90‑1 (MQ=255)                       ataATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTa                                                               <  1:1023576/90‑1 (MQ=255)                        taATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAg                                                              <  1:2395357/90‑1 (MQ=255)                        taATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAg                                                              >  1:1319716/1‑90 (MQ=255)                           tGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCa                                                           <  1:3822386/90‑1 (MQ=255)                           tGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCa                                                           <  1:1614605/90‑1 (MQ=255)                           tGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCa                                                           <  1:2908077/90‑1 (MQ=255)                             cgcATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAgg                                                         <  1:810169/90‑1 (MQ=255)                             cgcATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAgg                                                         <  1:2973192/90‑1 (MQ=255)                              gcATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGa                                                        <  1:3608865/90‑1 (MQ=255)                              gcATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGa                                                        <  1:978998/90‑1 (MQ=255)                              gcATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGa                                                        <  1:2088800/90‑1 (MQ=255)                                aTAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATg                                                      >  1:1886494/1‑90 (MQ=255)                                aTAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATg                                                      <  1:630244/90‑1 (MQ=255)                                  aGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATgac                                                    >  1:3049161/1‑88 (MQ=255)                                  aGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCa                                                    >  1:1624889/1‑90 (MQ=255)                                   gCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAg                                                   >  1:427964/1‑90 (MQ=255)                                    ccACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGt                                                  <  1:264499/90‑1 (MQ=255)                                     cacaAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGta                                                 >  1:636803/1‑90 (MQ=255)                                      acaAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGtaa                                                <  1:3992497/90‑1 (MQ=255)                                      acaAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGtaa                                                >  1:2554359/1‑90 (MQ=255)                                            ggCTGTTGTTAGTACTGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATc                                          <  1:2341550/90‑1 (MQ=255)                                             gCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATcc                                         <  1:4206323/90‑1 (MQ=255)                                             gCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATcc                                         >  1:4331824/1‑90 (MQ=255)                                                gttgttAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATccgcc                                      >  1:3900141/1‑90 (MQ=255)                                                    ttAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATccgccgccg                                  <  1:20995/90‑1 (MQ=255)                                                        tACCGGTAATCCTGCAGTAATCGTTTCCAGTCGCACAATTCCCGCTGCTGCCTGGTAGGCAGAATGCAGTAAAAAATCCGCCGCCGCCAt                              <  1:911008/90‑1 (MQ=255)                                                          ccGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTa                            <  1:3991062/90‑1 (MQ=255)                                                           cGGTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTaa                           <  1:3224403/90‑1 (MQ=255)                                                            ggTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAAt                          <  1:3944372/90‑1 (MQ=255)                                                            ggTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCAGCCGCCGCCATTAAt                          >  1:3448222/1‑90 (MQ=255)                                                             gTAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAAtt                         <  1:3192453/90‑1 (MQ=255)                                                              tAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAATTc                        <  1:1710629/90‑1 (MQ=255)                                                              tAATCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAATTc                        <  1:304362/90‑1 (MQ=255)                                                                aTCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAATTCCg                      >  1:178711/1‑90 (MQ=255)                                                                 tCCTGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAATTCCga                     <  1:454811/90‑1 (MQ=255)                                                                    tGCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAATTCCGAGAc                  <  1:3317612/90‑1 (MQ=255)                                                                     gCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAATTCCGAGACa                 <  1:1183311/90‑1 (MQ=255)                                                                     gCAGTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAATTCCGAGACa                 <  1:1788924/90‑1 (MQ=255)                                                                       aGTAATCGCTTCCAGACGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAATTCCGAGACATc               >  1:1079272/1‑90 (MQ=255)                                                                        gTAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATCCAGTAATAAATCCGCCGCCGCCATTAATTCCGAGACATCg              <  1:2386634/90‑1 (MQ=255)                                                                         tAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAATTCCGAGACATCGt             >  1:1937646/1‑90 (MQ=255)                                                                         tAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAATTCCGAGACATCGt             >  1:3106219/1‑90 (MQ=255)                                                                         tAATCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAATTCCGAGACATCGt             >  1:3463286/1‑90 (MQ=255)                                                                          aaTCGCTTCCAGTCGCTCAATTCCCGCTGCTTCCTGGAAGGCAGGATGCAGTAATAAAGCCGCCGCCGCCATTAATTCCGAGACATCGtt            <  1:171951/90‑1 (MQ=255)                                                                           aTCGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAATTCCGAGACATCGTTg           >  1:2630994/1‑90 (MQ=255)                                                                             cGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAATTCCGAGACATCGTTGCg         >  1:3893144/1‑90 (MQ=255)                                                                             cGCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAATTCCGAGACATCGTTGCg         <  1:363535/90‑1 (MQ=255)                                                                              gCTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAATTCCGAGACATCGTTGCGt        >  1:748347/1‑90 (MQ=255)                                                                               cTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAATTCCGAGACATCGTTGCGTc       >  1:2566537/1‑90 (MQ=255)                                                                               cTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAATTCCGAGACATCGTTGCGTc       <  1:2567315/90‑1 (MQ=255)                                                                               cTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAATTCCGAGACATCGTTGCGTc       >  1:1871177/1‑90 (MQ=255)                                                                               cTTCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAATTCCGAGACATCGTTGCGTc       >  1:1645140/1‑90 (MQ=255)                                                                                 tCCAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAATTCCGAGACATCGTTGCGTccc     >  1:469440/1‑90 (MQ=255)                                                                                  ccAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAATTCCGAGACATCGTTCCGTCCCg    >  1:277268/1‑90 (MQ=255)                                                                                   cAGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAATTCCGAGACATCGTTGCGTCCCga   <  1:1725607/90‑1 (MQ=255)                                                                                    aGTCGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAATTCCGAGACATCGTTGCGTCCCgag  >  1:609260/1‑90 (MQ=255)                                                                                       |                                                                                       CGCCGCAATTGGCGTCGACAATATAATGCGCATAGCCACAAACGGCTGTTGTTAGTACCGGTAATCCTGCAGTAATCGCTTCCAGTAGCACAATTCCCGCTGCTTCCTGGTAGGCAGGATGCAGTAATAAATCCGCCGCCGCCATTAATTCCGAGACATCGTTGCGTCCCGAG  >  NC_012967/3744482‑3744654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑