Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_012967	70,867	T→C	D92G (GAC→GGC)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012967	70,867	0	T	C	100.0%	292.4 / NA	73	D92G (GAC→GGC)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/41);  total (32/41)

ATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGTCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCG  >  NC_012967/70789‑70942                                                                               |                                                                            atatCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGGGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGt                                                                          >  1:520708/1‑82 (MQ=255)  tatCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGtt                                                                         >  1:1590343/1‑82 (MQ=255)   atCGATACTGTCCCACGGCAGCTCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTg                                                                        <  1:2150950/82‑1 (MQ=255)   atCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTg                                                                        <  1:3223333/82‑1 (MQ=255)   atCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTg                                                                        <  1:460032/82‑1 (MQ=255)    tCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGa                                                                       <  1:2733453/82‑1 (MQ=255)      gATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATc                                                                     <  1:2364482/82‑1 (MQ=255)      gATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATc                                                                     <  1:1588125/82‑1 (MQ=255)      gATACTGGCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATc                                                                     <  1:2759358/82‑1 (MQ=255)        tACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCca                                                                   >  1:1134333/1‑82 (MQ=255)         aCTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCcac                                                                  >  1:906205/1‑82 (MQ=255)         aCTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCcac                                                                  >  1:1294996/1‑82 (MQ=255)           tGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACAt                                                                <  1:4200497/82‑1 (MQ=255)           tGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACAt                                                                <  1:1113807/82‑1 (MQ=255)             tCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACAttt                                                              >  1:65153/1‑82 (MQ=255)             tCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACAttt                                                              <  1:1435597/82‑1 (MQ=255)              cccACGGCAGCGCCGCGTTGAACTGGGGGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACAtttt                                                             >  1:3329327/1‑82 (MQ=255)               ccACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTg                                                            >  1:2241627/1‑82 (MQ=255)               ccACGGCAGCGCCGCGTTGAACTGGGGGTGGAATTGCAGCAACCGTTTGTTGAGCATGGTCAAGCCGTTGATCCACATTTTg                                                            >  1:115822/1‑82 (MQ=255)                cACGGCAGCGCCGCGGTGAACTGGGTGGGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGGTGATCCACATTTTgg                                                           >  1:41093/1‑82 (MQ=255)                 ccGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGc                                                          <  1:2754186/81‑1 (MQ=255)                  cGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGcc                                                         <  1:2302201/82‑1 (MQ=255)                    gCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCgg                                                       >  1:494782/1‑82 (MQ=255)                    gCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCgg                                                       <  1:1028841/82‑1 (MQ=255)                    gCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCgg                                                       <  1:181616/82‑1 (MQ=255)                    gCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCgg                                                       >  1:980178/1‑82 (MQ=255)                     cAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCggg                                                      >  1:4043125/1‑82 (MQ=255)                      aGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCgggg                                                     <  1:3307202/82‑1 (MQ=255)                       gcgcCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGga                                                    >  1:3899311/1‑82 (MQ=255)                        cgccgcGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGgag                                                   >  1:2868383/1‑82 (MQ=255)                         gccgcGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCTGTTGATCCACATTTTGGCCGGGgaga                                                  <  1:442695/82‑1 (MQ=255)                         gccgcGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCTTTGATCCACATTTTGGCCGGGgaga                                                  <  1:3879428/82‑1 (MQ=255)                         gccgcGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGgaga                                                  <  1:1483895/82‑1 (MQ=255)                         gccgcGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGgaga                                                  <  1:2248438/82‑1 (MQ=255)                          ccgcGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCTTTGATCCACATTTTGGCCGGGGAGaa                                                 <  1:1290419/82‑1 (MQ=255)                          ccgcGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGaa                                                 >  1:3007740/1‑82 (MQ=255)                            gcgTTGAACTGGTTTTGGAATTGCAGCAACGGTTGGTGGAGCAGGGTCAGGCCTTTGATCCACATTTTGGCCGGGGAGAAgg                                               <  1:191831/82‑1 (MQ=255)                            gcgTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAgg                                               <  1:1241003/82‑1 (MQ=255)                            gcgTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAgg                                               <  1:1374062/82‑1 (MQ=255)                            gcgTTGAACTGGGGGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAgg                                               >  1:98187/1‑82 (MQ=255)                             cgTGGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGgt                                              <  1:1634499/82‑1 (MQ=255)                               ttGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGgtgt                                            >  1:4305807/1‑82 (MQ=255)                                tGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGtgtg                                           <  1:4252880/82‑1 (MQ=255)                                tGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGtgtg                                           <  1:3587060/82‑1 (MQ=255)                                  aaCTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCa                                         <  1:3825851/82‑1 (MQ=255)                                  aaCTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCa                                         >  1:4005994/1‑82 (MQ=255)                                    cTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGc                                       >  1:2391484/1‑82 (MQ=255)                                     tGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGcc                                      <  1:2621008/82‑1 (MQ=255)                                      gggTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCca                                     >  1:1690205/1‑82 (MQ=255)                                         tgtgGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCacac                                  >  1:2053364/1‑82 (MQ=255)                                          gtgGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCAcacc                                 <  1:646425/82‑1 (MQ=255)                                             gAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACaccacc                              >  1:3086063/1‑82 (MQ=255)                                             gAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACaccacc                              >  1:2784002/1‑82 (MQ=255)                                             gAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACaccacc                              >  1:1123238/1‑82 (MQ=255)                                               aTTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCaccag                            >  1:1284755/1‑82 (MQ=255)                                                 tGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCaccagac                          <  1:4312534/82‑1 (MQ=255)                                                    agcaACGGTTTTTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCaccagaccag                       >  1:3406079/1‑82 (MQ=255)                                                       aaCGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAgcgc                    >  1:2520970/1‑82 (MQ=255)                                                          ggTTTGTTGAGCATGTTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAAc                 <  1:523160/82‑1 (MQ=255)                                                           gTTTGTTGAGCATGTTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGTTGTGCAGCCACACCACCGGACCAGCGCAACg                <  1:4077736/82‑1 (MQ=255)                                                             ttgttgAGCATGGTCAGGCCGGTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGAt              >  1:1119897/1‑82 (MQ=255)                                                              tgttgAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGAtc             >  1:2316489/1‑82 (MQ=255)                                                              tgttgAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGAtc             <  1:1736360/82‑1 (MQ=255)                                                              tgttgAGCAGGGTCTGGCCGTTGATCCACATTTTGGCCGGGGATAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGAtc             <  1:2113835/82‑1 (MQ=255)                                                                ttgAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGAtcgt           >  1:2776771/1‑82 (MQ=255)                                                                ttgAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGAtcgt           <  1:3391440/82‑1 (MQ=255)                                                                ttgAGCATGGGCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGGGTGCAGCCACACCACCAGACCAGCGCAACGAtcgt           <  1:1729658/82‑1 (MQ=255)                                                                 tgAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGAtcgtc          <  1:4250282/82‑1 (MQ=255)                                                                 tgAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGAtcgtc          <  1:179452/82‑1 (MQ=255)                                                                   aGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATcgtcgt        <  1:413484/82‑1 (MQ=255)                                                                   aGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATcgtcgt        >  1:4280540/1‑82 (MQ=255)                                                                     cATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACTACCAGACCAGCGCAACGATCGTCGTaa      >  1:2692467/1‑82 (MQ=255)                                                                       tGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAAtt    <  1:2287422/82‑1 (MQ=255)                                                                       tGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAAtt    <  1:2290138/82‑1 (MQ=255)                                                                       tGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAAtt    <  1:2551844/82‑1 (MQ=255)                                                                         gTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATtcg  <  1:1911836/82‑1 (MQ=255)                                                                         gTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATtcg  <  1:1544779/82‑1 (MQ=255)                                                                               |                                                                            ATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGTCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCG  >  NC_012967/70789‑70942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑