breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsSRR630511_R1.good.fq4,367,714358,152,548100.0%82.0 bases82 bases60.0%
total4,367,714358,152,548100.0%82.0 bases82 bases60.0%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0129674,629,81250.17.9100.0%Escherichia coli B str. REL606, complete sequence.
total4,629,812100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 1000001200
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 2
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 50004
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.000

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0129670.77208

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction0.1
Junction allow suboptimal matchesFALSE
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.2.6
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input20:58:29 25 Jun 202320:59:48 25 Jun 20231 minute 19 seconds
Read alignment to reference genome20:59:48 25 Jun 202321:11:58 25 Jun 202312 minutes 10 seconds
Preprocessing alignments for candidate junction identification21:11:58 25 Jun 202321:12:51 25 Jun 202353 seconds
Preliminary analysis of coverage distribution21:12:51 25 Jun 202321:14:23 25 Jun 20231 minute 32 seconds
Identifying junction candidates21:14:23 25 Jun 202321:14:24 25 Jun 20231 second
Re-alignment to junction candidates21:14:24 25 Jun 202321:16:40 25 Jun 20232 minutes 16 seconds
Resolving best read alignments21:16:40 25 Jun 202321:18:08 25 Jun 20231 minute 28 seconds
Creating BAM files21:18:08 25 Jun 202321:19:29 25 Jun 20231 minute 21 seconds
Tabulating error counts21:19:29 25 Jun 202321:20:01 25 Jun 202332 seconds
Re-calibrating base error rates21:20:01 25 Jun 202321:20:03 25 Jun 20232 seconds
Examining read alignment evidence21:20:03 25 Jun 202321:25:49 25 Jun 20235 minutes 46 seconds
Polymorphism statistics21:25:49 25 Jun 202321:25:50 25 Jun 20231 second
Output21:25:50 25 Jun 202321:25:59 25 Jun 20239 seconds
Total 27 minutes 30 seconds