Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_012967	2,847,052	A→G	V10A (GTT→GCT)	recD ←	exodeoxyribonuclease V subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012967	2,847,052	0	A	G	100.0%	161.1 / NA	39	V10A (GTT→GCT)	recD	exodeoxyribonuclease V subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/20);  total (19/20)

GATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAACTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCA  >  NC_012967/2846984‑2847120                                                                     |                                                                     gATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTc                                                                  <  1:3358939/73‑1 (MQ=255)    gTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAg                                                               <  1:12425169/73‑1 (MQ=255)      tCATCTCCCGCCTCGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTa                                                             <  1:2780621/73‑1 (MQ=255)      tCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCTACAGCTTCCAGTa                                                             >  1:7321629/1‑73 (MQ=255)          ctcCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTg                                                         <  1:10572888/73‑1 (MQ=255)            cccGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCt                                                       >  1:11265250/1‑73 (MQ=255)             ccGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCtt                                                      >  1:10771222/1‑73 (MQ=255)                ccACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTg                                                   >  1:9362449/1‑73 (MQ=255)                ccACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTg                                                   <  1:3353606/73‑1 (MQ=255)                ccACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTg                                                   <  1:11993927/73‑1 (MQ=255)                   cGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCaa                                                >  1:10662891/1‑73 (MQ=255)                   cGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCaa                                                >  1:10662889/1‑73 (MQ=255)                     gTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAAtt                                              <  1:10442769/73‑1 (MQ=255)                     gTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAAtt                                              >  1:3055446/1‑73 (MQ=255)                      tCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAAttt                                             >  1:7713366/1‑73 (MQ=255)                           gCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTa                                        <  1:4567547/73‑1 (MQ=255)                           gCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTa                                        >  1:4612967/1‑73 (MQ=255)                            cAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCGCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTAc                                       <  1:13190751/73‑1 (MQ=255)                             aaaTTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACg                                      <  1:11909519/73‑1 (MQ=255)                               aTTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGcc                                    <  1:11348369/73‑1 (MQ=255)                                ttGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGcct                                   <  1:6691934/73‑1 (MQ=255)                                  gCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGcctcc                                 <  1:7284303/73‑1 (MQ=255)                                    acaTCCAGCGGGCGTAGCTGTTTGTGCGCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCctcctc                               <  1:6320646/73‑1 (MQ=255)                                           gCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTc                        >  1:3604062/1‑73 (MQ=255)                                            cGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCa                       <  1:4413660/73‑1 (MQ=255)                                                cGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATAcc                   <  1:8790689/73‑1 (MQ=255)                                                  tAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCgg                 >  1:6730059/1‑73 (MQ=255)                                                      tGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCaaa             >  1:12374648/1‑73 (MQ=255)                                                      tGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTGCATTACGCCTCCTCCAGGGTCATACCGGCaaa             <  1:1880857/73‑1 (MQ=255)                                                        tttGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACa           >  1:8950613/1‑73 (MQ=255)                                                          tgtgCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACAtc         >  1:9858438/1‑73 (MQ=255)                                                          tgtgCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACAtc         <  1:11927368/73‑1 (MQ=255)                                                           gtgCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACAtct        <  1:10377419/73‑1 (MQ=255)                                                            tgCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACAtctc       >  1:9831032/1‑73 (MQ=255)                                                             gCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTca      >  1:4347482/1‑73 (MQ=255)                                                               tCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTcatc    >  1:5129426/1‑73 (MQ=255)                                                               tCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTcatc    <  1:8228542/73‑1 (MQ=255)                                                                ccACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTcatcc   >  1:10387375/1‑73 (MQ=255)                                                                ccACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTcatcc   >  1:10387372/1‑73 (MQ=255)                                                                 cacaGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTcatcca  <  1:7216416/73‑1 (MQ=255)                                                                     |                                                                     GATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAACTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCA  >  NC_012967/2846984‑2847120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑