Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451	278,744	+A	intergenic (‑239/+75)	PP_0224 ← / ← sctC	Monooxygenase, DszC family/sulfur compound ABC transporter ‑ ATP‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	278,742	1	.	A	100.0%	17.2 / NA	6	intergenic (‑237/+77)	PP_0224/sctC	Monooxygenase, DszC family/sulfur compound ABC transporter ‑ ATP‑binding subunit
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (5/1);  total (5/1)

GTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGT‑AACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTGGCCTCGCCTTGCTCCACGAT  >  AE015451/278638‑278920                                                                                                          |                                                                                                                                                                                   gtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAgg                                                                                             <  1:154738/193‑1 (MQ=255) gtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAgg                                                                                             >  2:154737/1‑193 (MQ=255)               cccTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTg                                                                                                                                             >  2:322091/1‑131 (MQ=255)                      gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGTGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTgc                                                                        >  2:433762/1‑193 (MQ=255)                                                                          tttGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTGGCCTCGCCTTGCTCCACGAt  >  2:162291/1‑211 (MQ=255)                                                                                         ccGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGctct                                                 >  1:417342/1‑149 (MQ=255)                                                                                                          |                                                                                                                                                                                   GTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGT‑AACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTGGCCTCGCCTTGCTCCACGAT  >  AE015451/278638‑278920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑