Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451	3,845,705	+G	intergenic (‑72/+26)	PP_3394 ← / ← PP_3395	putative 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/Transcriptional regulator, LysR family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	3,845,703	1	.	G	100.0%	77.8 / NA	23	intergenic (‑70/+28)	PP_3394/PP_3395	putative 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/Transcriptional regulator, LysR family
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (11/12);  total (11/12)

GACGCGACTTCCATATGGCGCACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑GGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCC  >  AE015451/3845497‑3845887                                                                                                                                                                                                                |                                                                                                                                                                                         gacgCGACTTCCTTATGGCGCACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTGGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATg                                                                                                                                                                 <  1:308621/233‑1 (MQ=255)                 ggCGCACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCa                                                                                                                                             <  1:409676/237‑1 (MQ=255)                  gcgcACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAg                                                                                                                                            <  1:364353/237‑1 (MQ=255)                      aCGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCNTCGTCAGCCNCg                                                                                                                                                                    >  1:28961/1‑209 (MQ=255)                                cGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCGGATCCATCGTCAGCCACGATGGATCAGCCcgcg                                                                                                                                                     >  1:375081/1‑214 (MQ=255)                                   aaGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCaa                                                                                                                           <  1:176612/237‑1 (MQ=255)                                              aTCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCa                                                                                                                 >  1:200285/1‑236 (MQ=255)                                                cnAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCaa                                                                                                                           <  1:98162/224‑1 (MQ=255)                                                   gcggcgTTTGGCTGCTGTCGGCATGATCACGTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGccc                                                                                                                                                        <  2:271599/192‑1 (MQ=255)                                                            ggCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGACCAGCCCCCGCCCTGcccg                                                                                                                                            >  1:227269/1‑193 (MQ=255)                                                             gctgctGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCGCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAgg                                                                                                           <  2:205834/227‑1 (MQ=255)                                                                                              aaGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACTGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgcc                                                                                                       >  1:51902/1‑198 (MQ=255)                                                                                              aaGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGCGCACGCAGCAAccg                                                                                                                        >  2:38074/1‑179 (MQ=255)                                                                                                               gACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCACCAGGTGGTCAAGCAGGCCCCGAGCCGACAGc                                                                                            >  1:398537/1‑192 (MQ=255)                                                                                                               gACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCaa                                                 >  1:238785/1‑235 (MQ=255)                                                                                                                                                    ctgtctgAAGCGTGGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTc            <  1:299422/235‑1 (MQ=255)                                                                                                                                                            aGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAAcgcc  >  1:390526/1‑237 (MQ=255)                                                                                                                                                                   cgcCAGACTAAGAGCTGGATGTCGGGCAGAAAAGACATGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCga                                                                                          <  1:378474/142‑1 (MQ=255)                                                                                                                                                                    gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAgtcgtc                                    >  1:26704/1‑195 (MQ=255)                                                                                                                                                                           taggagCTGGATGACGGGCAGAAAAGACGTGTGTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGGAGCAACAggt                                                                                                                      <  2:138873/103‑1 (MQ=255)                                                                                                                                                                           tAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgagccg                                                                                                 >  1:412927/1‑127 (MQ=255)                                                                                                                                                                           tAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgagccg                                                                                                 <  2:412925/127‑1 (MQ=255)                                                                                                                                                                                                      cGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAg                                                                                                                                            <  2:260309/57‑1 (MQ=255)                                                                                                                                                                                                                |                                                                                                                                                                                         GACGCGACTTCCATATGGCGCACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑GGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCC  >  AE015451/3845497‑3845887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑