Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	AE015451	5,555,608	+GCC	intergenic (‑84/+87)	PP_4887 ← / ← PP_4888	conserved protein of unknown function/putative methyl‑accepting chemotaxis transducer

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 5555608	0 (0.000)	25 (1.140) +GCC	23/310	0.1	100%	intergenic (‑84/+87)	PP_4887/PP_4888	conserved protein of unknown function/putative methyl‑accepting chemotaxis transducer
	?	AE015451	5555609 =	0 (0.000)					intergenic (‑85/+86)	PP_4887/PP_4888	conserved protein of unknown function/putative methyl‑accepting chemotaxis transducer

GAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGCTACTGGCCGTGGTCTGCATCACCACCTCAGCCGAGCGTTCGGCCTGGGCATGG  >  AE015451/5555378‑5555809                                                                                                                                                                                                                                       |                                                                                                                                                                                                          gAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGgccccg                                                                                                                                                                                                     <  1:156418‑M1/237‑7 (MQ=255)      cccGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGgccccggcaat                                                                                                                                                                                                <  1:143830‑M1/237‑12 (MQ=255)                                         gcgcAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACACCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGgccccggcaatttccgcgtgtatgcaacaaacctggggctgctat                                                                                                                                                              >  1:370764‑M1/1‑191 (MQ=255)                                                       cAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGgccccggcaa                                                                                                                                                                                                 >  2:433338‑M1/1‑177 (MQ=255)                                                           gCGGTCAGCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATGCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACAGGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGgccccggcaatttccgcgtgtatgcaacaaacctgggg                                                                                                                                                                     <  2:410102‑M1/211‑39 (MQ=255)                                                                          gTGAGCCGTACGCTGTCACTGTGCAGGAAATTCGGGTCGGCCTGGGCTGCGCTTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGgccccggcaatttccgcgtgtatgcaacaaacc                                                                                                                                                                          <  1:145900‑M1/191‑34 (MQ=255)                                                                                            cTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGgcgccggcaatttccgcgtgtatgcaacaaacctggggctgctattcagcacgttcgctacacaaggccc                                                                                                                                     >  1:402154‑M1/1‑140 (MQ=255)                                                                                                   gnAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGgccccggcaatttccgcgtgtatgcaacaaacctggggctgctatgcagccctttcgcgacacaa                                                                                                                                          <  1:76992‑M1/198‑66 (MQ=255)                                                                                                          cAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGgccccggcaatttccgcgtgtatgcaacaaacctggggctgct                                                                                                                                                                >  1:320056‑M1/1‑126 (MQ=255)                                                                                                                             aTTAGGCGGGCCGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGgccccggcaatttccgcgtgtatgcaacaaacctggggctgctatgcagccctttcgcgacacaaggcccacaaaggaccgcgcaggcttcaggcccggaactggcccaggctggcgcgcagctgagccg                                                                         <  1:144750‑M1/237‑131 (MQ=255)                                                                                                                                                                                    aaCACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGgccccggcaatttccgcgtgtatgcaacaaacctggggctgctatgcagccctttcgcgacac                                                                                                                                            <  1:114632‑M1/115‑64 (MQ=255)                                                                                                                                                                                    aaCACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGgccccggcaatttccgcgtgtatgcaacaaacctggggctgctatgcagccctttcgcgacac                                                                                                                                            >  2:114631‑M1/1‑52 (MQ=255)                                                                                                                                                                                         cGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGTGCCGCATAGCGgccccggcaatttccgcgtgcatgcaacaaacctggggctgctatgcagccctttcgcgacacaaggcccacaaagga                                                                                                                             >  1:25167‑M1/1‑47 (MQ=255)                                                                                                                                                                                           cTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGgccccggcaatttccgcgtgtatgcaacaaacctggggctgctatgcagccc                                                                                                                                                       <  1:342859‑M1/97‑53 (MQ=255)                                                                                                                                                                                           cTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGgccccggcaatttccgcgtgtatgcaacaaacctggggctgctatgcagccc                                                                                                                                                       >  2:342857‑M1/1‑45 (MQ=255)                                                                                                                                                                                                    gggAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGgccccggcaatttccgcgtgtatgcaacaaacctggggctgctatgcagccctttcgcgacacaaggcccacaaaggaccgcgcaggcttcaggcccggaactggcccaggctggcgcgcagctgagccgccagatcatccagcaccttgctactggccgtggtctgcatcaccacctcagccgagcgttcggcctgggca  <  1:267625‑M1/237‑202 (MQ=255)                                                                                                                                                                                                         cGGCCTTGTGTCGCGAAAGGGCCGCATAGCGgccccggcaatttccgcgtgtatgcaacaaacctggggctgctatgcagccctttcgcgacccaaggcccacaaaggacc                                                                                                                           <  2:122385‑M1/111‑81 (MQ=255)                                                                                                                                                                                                             cTTGTGTCGCGAAAGGGCCGCATAGCGgccccggcaatttccgcgtgtatgcaacaaacctggggctgctatgcagccctttcgcgacacaaggcccacaaaggaccgcgcaggcttcaggcccggaactggcccaggctggcgcgcagctgggcccccagatcatccagcaccttgctactggccgtggtctgcatcaccacctcagccgagcgtt             >  1:406299‑M1/1‑27 (MQ=255)                                                                                                                                                                                                             cTTGTGTCGCGAAAGGGCCGCATAGCGgccccggcaatttccgcgtgtatgcaacaaacctggggctgctatgcagccctttcgcgacacaaggcccacaaaggaccgcgcaggcttcaggcccggaactggcccaggctggcgcgcagctgagccgcca                                                                      <  2:209057‑M1/160‑134 (MQ=255)                                                                                                                                                                                                             cTTGTGTCGCGAAAGGGCCGCATAGCGgccccggcaatttccgcgtgtatgcaacaaacctggggctgctatgcagccctttcgcgacacaaggcccacaaaggaccgcgcaggcttcaggcccggaactggcccaggctggcgcgcagctgagccgcca                                                                      >  1:209058‑M1/1‑27 (MQ=255)                                                                                                                                                                                                             cTTGTGTCGCGAAAGGGCCGCATAGCGgccccggcaatttccgcgtgtatgcaacaaacctggggctgctatgcagccctttcgcgacacaaggcccacaaaggaccgcgcaggcttcaggcccggaactg                                                                                                   >  1:376390‑M1/1‑27 (MQ=255)                                                                                                                                                                                                             cTTGTGTCGCGAAAGGGCCGCATAGCGgccccggcaatttccgcgtgtatgcaacaaacctggggctgctatgcagccctttcgcgacacaaggcccacaaaggaccgcgcaggcttcaggcccggaactg                                                                                                   <  2:376388‑M1/131‑105 (MQ=255)                                                                                                                                                                                                                       gacagggCCGCATAGCGgccccggcaatttccgcgtgtatgcaccaaacctggggctgctatgcagccctttcgctacacaaggcccacaaaggaccgcgcaggcttcaggcccggaactggcccaggctggcgcgcagctgagccgccagatcatccagcaccttgctactggccgtggt                                       <  1:144659‑M1/178‑165 (MQ=255)                                                                                                                                                                                                                         aaGGGCCGCATAGCGgccccggcaatttccgcgtgtatgcaacaaacctggggctgctatgcagccctttcgcgccacaaggcccacaaaggaccgcgcaggcttcaggcccggaactggcccaggccggcgcgcagctgagccgccag                                                                     >  2:256306‑M1/1‑15 (MQ=255)                                                                                                                                                                                                                                gCATAGCGgccccgggaatttccgcgtgtatgcaacaaacctggggctgctatgcagccctttcgcgacacaaggcccacaaaggaccgcgcaggcttcaggcccggaactggcccaggctggcgcgcagctgagccgccagatcatcca                                                             <  1:206853‑M1/150‑143 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                          GAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCGCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGCTACTGGCCGTGGTCTGCATCACCACCTCAGCCGAGCGTTCGGCCTGGGCATGG  >  AE015451/5555378‑5555809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.