| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 5,988,911 | 0 | A | . | 22.2% | 48.1 / 10.2 | 18 | intergenic (+205/‑341) | kefB‑III/PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA |
| Reads supporting (aligned to +/- strand): ref base A (10/4); new base . (1/3); total (11/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGC > AE015451/5988745‑5989086 | gagaTTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAg < 2:401364/180‑1 (MQ=255) aaCCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGACACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcg < 1:272984‑M2/180‑26 (MQ=255) tACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGc > 2:347409/1‑216 (MQ=255) tCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGTCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCggg > 2:328317/1‑131 (MQ=255) gggCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGc > 1:32015/1‑212 (MQ=255) cgcACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaaac > 2:216906/1‑77 (MQ=255) cgcACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGACGCGCCGCGCGGGCGGCGCTCGATCTCAt > 1:120329/1‑128 (MQ=255) gCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAg < 1:347411/237‑1 (MQ=255) gcggcgTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTa < 1:370726/63‑1 (MQ=11) aaacaaGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGCAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCCTGGGCGCCCaa > 2:435437/1‑113 (MQ=255) gTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgt < 1:261578‑M2/138‑83 (MQ=255) gTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgt > 2:261576‑M2/1‑56 (MQ=255) gcgcgcCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGa > 1:15062/1‑120 (MQ=255) gggATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGcc > 1:66380/1‑92 (MQ=255) gggATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGcc < 2:66379/92‑1 (MQ=255) ttGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGCCGCGCACTCACAAAAACCCTCACAAATCGCCTa > 2:112570/1‑173 (MQ=255) tGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccactcaaaattccaacgccgccccctcaaaattccaacgccgcctcccctcgaaaatccaacaacaaa < 2:368776‑M2/180‑152 (MQ=255) ggCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCACTTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAAcc > 2:436377/1‑156 (MQ=255) | GAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGC > AE015451/5988745‑5989086 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |