Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154511,843,9010CT36.0% 20.8 / 23.2 25R298H (CGC→CAC) gacSSensor protein GacS
Reads supporting (aligned to +/- strand):  ref base C (6/10);  new base T (3/6);  total (9/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGCCGGAGCGAGGATGGTCAGGGTGTCCTGGATCAGGTCCCGCAGGTTGAACGGTATGCTGTCGAGCACCAGCTTGCCGGCCTCGATCTTGGAGAAATCGAGGATCTCGTTGATGATTCCCAGCAAGTTGTCAGCAGACTTCTCGATGGTGTTCAGGTAGTCGAGCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCA  >  AE015451/1843669‑1844087
                                                                                                                                                                                                                                        |                                                                                                                                                                                          
ggcCGGAGCGAGGATGGTCAGGGTGTCCTGGATCAGGTCCCGCAGGTTGAACGGTATGCTGTCGAGCACCAGCTTGCCGGCCTCGATCTTGGAGAAATCGAGGATCTCGTTGATGATTCCCAGCAAGTTGTCAGCAGACTTCTCGATGGTGTTCAGGTAGTCGAGCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGAt                                                                                                                                                                                        >  1:264288/1‑237 (MQ=255)
                                     tCCCGCAGGTTGAACGGTATGCTGTCGAGCACCAGCTTGCCGGCCTCGATCTTGGAGAAATCGAGGATCTCGTTGATGATTCCCAGCAAGTTGTCAGCAGACTTCTCGATGGTGTTCAGGTAGTCGAGCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATg                                                                                                                                                   <  2:268881/237‑1 (MQ=255)
                                     tCCCGCAGGTTGAACGGTATGCTGTCGAGCACCAGCTTGCCGGCCTCGATCTTGGAGAAATCGAGGATCTCGTTGATGATTCCCAGCAAGTTGTCAGCAGACTTCTCGATGGTGTTCAGGTAGTCGAGCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATg                                                                                                                                                   <  1:352585/237‑1 (MQ=255)
                                      cccGCAGGTTGAACGGTATGCTGTCGAGCACCAGCTTGCCGGCCTCGATCTTGGAGAAATCGAGGATCTCGTTGATGATTCCCAGCAAGTTGTCAGCAGACTTCTCGATGGTGTTCAGGTAGTCGAGCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGc                                                                                                                                                  <  2:193251/237‑1 (MQ=255)
                                      cccGCAGGTTGAACGGTATGCTGTCGAGCACCAGCTTGCCGGCCTCGATCTTGGAGAAATCGAGGATCTCGTTGATGATTCCCAGCAAGTTGTCAGCAGACTTCTCGATGGTGTTCAGGTAGTCGAGCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGc                                                                                                                                                  >  1:329797/1‑237 (MQ=255)
                                       ccGCAGGTTGAACGGTATGCTGTCGAGCACCAGCTTGCCGGCCTCGATCTTGGAGAAATCGAGGATCTCGTTGATGATTCCCAGCAAGTTGTCAGCAGACTTCTCGATGGTGTTCAGGTAGTCGAGCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGTGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCg                                                                                                                                                 >  1:354276/1‑237 (MQ=255)
                                                           tGTCGAGCACCAGCTTGCCGGCCTCGATCTTGGAGAAATCGAGGATCTCGTTGATGATTCCCAGCAAGTTGTCAGCAGACTTCTCGATGGTGTTCAGGTAGTCGAGCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCtt                                                                                                                             <  1:245050/237‑1 (MQ=255)
                                                                    ccAGCTTGCCGGCCTCGATCTTGGAGAAATCGAGGATCTCGTTGATGATTCCCAGCAAGTTGTCAGCAGACTTCTCGATGGTGTTCAGGTAGTCGAGCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTgg                                                                                                                                                                       <  2:227377/186‑1 (MQ=255)
                                                                                    gATCTTGGAGAAATCGAGGATCTCGTTGATGATTCCCAGCAAGTTGTCAGCAGACTTCTCGATGGTGTTCAGGTAGTCGAGCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGTGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCATCTTTGCGCGCCATGTccctg                                                                                                            >  1:143064/1‑226 (MQ=255)
                                                                                       cTTGGAGAAATCGAGGATCTCGTTGATGATTCCCAGCAAGTTGTCAGCAGACTTCTCGATGGTGTTCAGGTAGTCGAGCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGTGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCtt                                                                                                                             <  2:143063/209‑1 (MQ=255)
                                                                                                   gAGGATCTCGTTGATGATTCCCAGCAAGTTGTCAGCAGACTTCTCGATGGTGTTCAGGTAGTCGAGCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGc                                                                                                                                                                                >  2:153043/1‑146 (MQ=255)
                                                                                                                                 gTCAGCAGACGTCTCGATGGTGTTCAGGTAGTCGAGCTGGCGCGGTGTCATCTCGCTGTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGTGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCtt                                                                                                                             <  2:72861/167‑1 (MQ=255)
                                                                                                                                            tctcGATGGTGTTCAGGTAGTCGAGCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGTGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGgcgc                                                                     >  1:35824/1‑212 (MQ=255)
                                                                                                                                                                  gAGCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTc                                                                                                                                                             <  2:160213/102‑1 (MQ=255)
                                                                                                                                                                  gAGCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTc                                                                                                                                                             >  1:160214/1‑102 (MQ=255)
                                                                                                                                                                  gAGCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGc                      >  1:246247/1‑237 (MQ=255)
                                                                                                                                                                       ggagcgGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGTGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATg                                             <  2:422765/206‑1 (MQ=255)
                                                                                                                                                                        gcgcGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGTGGATTTCTTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCg                                                <  1:223334/205‑1 (MQ=255)
                                                                                                                                                                                  aTCTCGCTTTGTTGCAGCAGGGGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGTGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTc                  <  1:219511/225‑1 (MQ=255)
                                                                                                                                                                                     tcGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCa                                                                                                                                                                             >  1:230868/1‑67 (MQ=255)
                                                                                                                                                                                     tcGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCa                                                                                                                                                                             <  2:230867/67‑1 (MQ=255)
                                                                                                                                                                                                   cagGTGGGTAAAGCCGAGGATGTCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGTCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCa  <  2:243704/224‑1 (MQ=255)
                                                                                                                                                                                                                      aTGCCGTTGAGCGGGGTGTGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTg                         <  2:35824/182‑1 (MQ=255)
                                                                                                                                                                                                                            tttaGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGACCTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTcc                                                                                 <  2:62514/117‑1 (MQ=255)
                                                                                                                                                                                                                            ttGAGCGGGGTGCGGATTTCGTGGCTCATGTTTGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTg                                     <  2:360800/164‑1 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                          
GGCCGGAGCGAGGATGGTCAGGGTGTCCTGGATCAGGTCCCGCAGGTTGAACGGTATGCTGTCGAGCACCAGCTTGCCGGCCTCGATCTTGGAGAAATCGAGGATCTCGTTGATGATTCCCAGCAAGTTGTCAGCAGACTTCTCGATGGTGTTCAGGTAGTCGAGCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCA  >  AE015451/1843669‑1844087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: