Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154514,348,7050AG46.9% 10.0 / 43.6 32intergenic (‑70/+404)PP_3820/galUGroup II intron‑encoding maturase/UTP‑glucose‑1‑phosphate uridylyltransferase
Reads supporting (aligned to +/- strand):  ref base A (8/9);  new base G (5/10);  total (13/19)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.47e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CGCTGACGCCGCCGGCACCTTTGCACTGTCAGGCATCACCCTCGGCTTCTGGCCGGGATTCGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCCCGCTGTGCCGCCTCTATCCGCTTCCTGTTCGTCAGGCCAGCATTTTGCCTCGGGCTTCCTTCAGATTCGCGGTCACCCGC  >  AE015451/4348501‑4348934
                                                                                                                                                                                                            |                                                                                                                                                                                                                                     
cGCTGACGCCGCCGGCACCTTTGCACTGTCAGGCATCACCCTCGGCTTCTGGCCGGGATTCGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGt                                                                                                                                                                                                       <  1:225624/237‑1 (MQ=11)
    gACGCCGCCGGCACCTTTGCACTGTCAGGCATCACCCTCGGCTTCTGGCCGGGATTCGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTgg                                                                                                                                                                                                     <  2:430827/235‑1 (MQ=31)
                                      cccTCGGCTTCTGGCCGGGATTCGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGAcc                                                                                                                                                                 <  1:374048/237‑1 (MQ=17)
                                                                     aGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTAc                                                                                                                                                                                            <  1:426583/179‑1 (MQ=33)
                                                                     aGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTAc                                                                                                                                                                                            >  2:426581/1‑179 (MQ=33)
                                                                          ccGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGCCGGCGCCGCCTACTGGCGGCATACCCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTc                                                                                                                                                                         <  1:114835/193‑1 (MQ=16)
                                                                              tgtgGGAAATGTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTAc                                                                                                                                                                                            <  2:312605/170‑1 (MQ=18)
                                                                                                tACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTCTTGTGCttt                                                                                                        >  1:301412/1‑236 (MQ=17)
                                                                                                                      aCGCCTACTGGCGGCATACCCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAg                                                                                                                                                                                                        <  2:116142/118‑1 (MQ=2)
                                                                                                                            aCTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATTTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGACTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCCCGCtgtg                                                                           >  1:177431/1‑237 (MQ=17)
                                                                                                                               ggcggcATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTcgcgc                                                                                                                                                                     <  1:234727/144‑1 (MQ=21)
                                                                                                                                     aTAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACCGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCCCACCCCGCTGTGCCGCctct                                                                   >  1:307539/1‑236 (MQ=17)
                                                                                                                                          cTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTtg                                                                                               <  2:259606/203‑1 (MQ=32)
                                                                                                                                                ggCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAg                                                                                                                                                                                                        >  1:13038/1‑92 (MQ=14)
                                                                                                                                                ggCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAg                                                                                                                                                                                                        <  1:390525/92‑1 (MQ=31)
                                                                                                                                                ggCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAg                                                                                                                                                                                                        >  2:390523/1‑92 (MQ=31)
                                                                                                                                                    gaggcaggCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCCCGCtg                                                                             <  1:35177/205‑1 (MQ=17)
                                                                                                                                                    cTTGGTGGCTCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCATCGCCATTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGAcc                                                                                                                                                                 <  2:352149/127‑1 (MQ=255)
                                                                                                                                                          ggCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCAc                                                                                    <  1:124310/198‑1 (MQ=18)
                                                                                                                                                           gcgcGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGc                                                                                                                               <  1:6369/154‑1 (MQ=33)
                                                                                                                                                               ggTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCtt                                                                                                                                                        >  2:307879/1‑125 (MQ=25)
                                                                                                                                                               ggTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGt                                                                                                                  >  2:1803/1‑163 (MQ=18)
                                                                                                                                                                    aCCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGt                                                                                                                                                                                                       <  1:27854/73‑1 (MQ=17)
                                                                                                                                                                        ccACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCCCGCGGTGCCGCCTCTATCCGCTTCCTGTTCGTCAGGCCAGCATTTTGCCTc                               <  1:172388/237‑1 (MQ=17)
                                                                                                                                                                         cACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCAcccc                                                                                 >  2:35177/1‑186 (MQ=33)
                                                                                                                                                                              actacGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCTCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGCTTTGTGCCACCTCACCCCGCTGTgccgc                                                                       >  2:220717/1‑191 (MQ=18)
                                                                                                                                                                              actacGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTCCAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGACCTACGTCACAGCGTTCCGTGCAAGTCTTg                                                                                                              >  2:222697/1‑152 (MQ=16)
                                                                                                                                                                                 acGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCg                                                                                                                         >  1:135385/1‑138 (MQ=21)
                                                                                                                                                                                           cTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCCCCCTGTGCCGCCTCTATCCGCTTCCTGTTCGTCAg                                               >  1:105490/1‑202 (MQ=17)
                                                                                                                                                                                            tGACTTCTGCACGCGCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGCTCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCAcc                                                                                        <  2:323326/160‑1 (MQ=18)
                                                                                                                                                                                                     ctcGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCt                                                                                                                                         <  2:3131/100‑1 (MQ=255)
                                                                                                                                                                                                     cACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCCCGCTGTGCCGCCTCTATCCGCTTCCTGTTCGTCAGGCCAGCATTTTGCCTCGGGCTTCCTTCAGATTCGCGGTCACCcgc  <  1:230922/237‑1 (MQ=17)
                                                                                                                                                                                                            |                                                                                                                                                                                                                                     
CGCTGACGCCGCCGGCACCTTTGCACTGTCAGGCATCACCCTCGGCTTCTGGCCGGGATTCGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCCCGCTGTGCCGCCTCTATCCGCTTCCTGTTCGTCAGGCCAGCATTTTGCCTCGGGCTTCCTTCAGATTCGCGGTCACCCGC  >  AE015451/4348501‑4348934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑