| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 74,493 | C→T | 6.8% | intergenic (+81/+17) | PP_0063 → / ← PP_0064 | putative Lipid A biosynthesis lauroyl acyltransferase/conserved protein of unknown function |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 74,493 | 0 | C | T | 6.8% | 194.6 / 4.6 | 59 | intergenic (+81/+17) | PP_0063/PP_0064 | putative Lipid A biosynthesis lauroyl acyltransferase/conserved protein of unknown function |
| Reads supporting (aligned to +/- strand): ref base C (32/23); new base T (2/2); total (34/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 | |||||||||||
TGCGCCGGAAGAGATGTACAGCACCGATGTGAATGTGTCGGCGGCGGCGATGAGCAAGGTGGTCGAACGCTATGTGCGCGAGTACCCCAGCCAGTACATGTGGAGCATGAAGCGCTTCAAGAAGCGCCCGGCTGGCGAGCCGCGCTGGTATTAAGCCATTCACCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTCGCCCTGCAGCTGATAGGCCTTACCCAGCAGCTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTT > AE015451/74259‑74724 | tGCGCCGGAAGAGATGTACAGCACCGATGTGAATGTGTCGGCGGCGGCGATGAGCAAGGTGGTCGAACGCTATGTGCGCGAGTACCCCAGCCAGTACATGTGGAGCATGAAGCGCTTCAAGAAGCGCCCGGCTGGCGAGCCGCGCTGGTATTAAGCCATTCACCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGTCGTATTCGCGGGTAAACCCGCt > 1:503706/1‑236 (MQ=255) cGATGTGAATGTGTCGGCGGCGGCGATGAGCAAGGTGGTCGAACGCTATGTGCGCGAGTACCCCAGCCAGTACATGTGGAGCATGAAGCGCTTCAAGAAGCGCCCGGCTGGCGAGCCGCGCTGGTATTAAGCCATTCACCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGa > 1:404017/1‑236 (MQ=255) tgAATGTGTCGGCGGCGGCGATGAGCAAGGTGGTCGAACGCTATGTGCGCGAGTACCCCAGCCAGTACATGTGGAGCATGAAGCGCTTCAAGAAGCGCCCGGCTGGCGAGCCGCGCTGGTATTAAGCCATTCACCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGAAAACCCGCTCCGACAGGGACCGAGTCAAGCCGATGtg > 1:139090/1‑234 (MQ=255) gtgtCGGCGGCGGCGATGAGCAAGGTGGTCGAACGCTATGTGCGCGAGTACCCCAGCCAGTACATGTGGAGCATGAAGCGCTTCAAGAAGCGCCCGGCTGGCGAGCCGCGCTGGTATTAAGCCATTCACCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGtt > 1:161573/1‑235 (MQ=255) aTGAGCAAGGTGGTCGAACGCTATGTGCGCGAGTACCCCAGCCAGTACATGTGGAGCATGAAGCGCTTCAAGAAGCGCCCGGCTGGCGAGCCGCGCTGGTATTAAGCCATTCACCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTgaga < 2:328677/233‑1 (MQ=255) gcgcGAGTACCCCAGCCAGTACATGTGGAGCATGAAGCGCTTCAAGAAGCGCCCGGCTGGCGAGCCGCGCTGGTATTAAGCCATTCACCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGCCCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAACAACGGTCATTCCTTTTTCGGCCTGc > 1:883472/1‑234 (MQ=255) gcgcGAGTACCCCAGCCAGTACATGTGGAGCATGAAGCGCTTCAAGAAGCGCCCGGCTGGCGAGCCGCGCTGGTATTAAGCCATTCACCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTg > 2:818814/1‑233 (MQ=255) gAGTACCCCAGCCAGTACATGTGGAGCATGAAGCGCTTCAAGAAGCGCCCGGCTGGCGAGCCGCGCTGGTATTAAGCCATTCACCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCa > 1:444033/1‑236 (MQ=255) aCCCCAGCCAGTACATGTGGAGCATGAAGCGCTTCAAGAAGCGCCCGGCTGGCGAGCCGCGCTGGTATTAAGCCATTCACCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCa > 1:516673/1‑235 (MQ=255) cagccagTACATGTGGAGCATGAAGCGCTTCAAGAAGCGCCCGGCTGGCGAGCCGCGCTGGTATTAAGCCATTCACCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGTTc < 1:108863/150‑1 (MQ=255) cagccagTACATGTGGAGCATGAAGCGCTTCAAGAAGCGCCCGGCTGGCGAGCCGCGCTGGTATTAAGCCATTCACCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGTTc > 2:108862/1‑150 (MQ=255) cagccagTACATGTGGAGCATGAAGCGCTTCAAGAAGCGCCCGGCTGGCGAGCCGCGCTGGTATTAAGCCATTCACCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGCAAAACGGTCATTTCTTTTTCGGCCTTCTTGTCTCCAttg > 2:484326/1‑232 (MQ=255) cagccagTACATGTGGAGCATGAAGCGCTTCAAGAAGCGCCCGGCTGGCGAGCCGCGCTGGTATTAAGCCATTCACCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGc > 1:895964/1‑236 (MQ=255) gagaATGAAGCGCTTCAAGAAGCGCCCGGCTGGCGAGCCGCGCTGGTATTAAGCCATTCACCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGctt < 2:617890/173‑1 (MQ=255) aTGAAGCGCTTCAAGAAGCGCCCGGCTGGCGAGCCGCGCTGGTATTAAGCCATTCACCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTg < 1:862384/206‑1 (MQ=255) aGCGCTTCAAGAAGCGCCCGGCTGGCGAGCCGCGCTGGTATTAAGCCATTCACCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGt > 2:581309/1‑233 (MQ=255) cgcTTCAAGAAGCGCCCGGCTGGCGAGCCGCGCTGGTATTAAGCCATTCACCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTcc > 1:110880/1‑236 (MQ=255) tCAAGAAGCGCCCGGCTGGCGAGCCGCGCTGGTATTAAGCCATTCACCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACg > 2:260859/1‑172 (MQ=255) aagaagCGCCCGGCTGGCGAGCCGCGCTGGTATTAAGCCATTCACCGTCCTCTGTAGGAACGGGTTTACCCGCGAATACGTCGGTGAATCCAACATCGCATTCGCGGGTAAACCCGTTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTc < 2:534894/184‑1 (MQ=25) aagaagCGCCCGGCTGGCGAGCCGCGCTGGTATTAAGCCATTCACCGTCCTCTGTAGGAACGGGTTTACCCGCGAATACGTCGGTGAATCCAACATCGCATTCGCGGGTAAACCCGTTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTc > 1:534897/1‑184 (MQ=25) ggTATTAAGCCATTCACCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTCGCCCTg < 2:404014/234‑1 (MQ=255) tATTAAGCCATTCACCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCg < 2:697653/155‑1 (MQ=255) cACCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTgcgcgc > 2:704155/1‑202 (MQ=255) cACCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGAAGTTGGTTTCACCGAGGTATTCGCGGGTGAATCCGc > 2:345097/1‑75 (MQ=25) aCCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGCAAAACGGTCCTTTCTTTTTCGGCCTGCTTGTCCCCCTGGTCCTGCGCCGCCACGCTCCCGTCTTCCCACGCttct > 2:230064/1‑194 (MQ=39) aCCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCt < 1:935426/184‑1 (MQ=255) aCCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCt > 2:935423/1‑184 (MQ=255) cGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGc < 2:557721/209‑1 (MQ=255) ctctGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCGGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATccc < 2:568882/174‑1 (MQ=255) tctGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTCGCCCTGCAGCTGATAGGCCTTACCcag > 2:892503/1‑233 (MQ=255) gTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAACCCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCt < 2:684751/139‑1 (MQ=255) gTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGc > 1:47701/1‑152 (MQ=255) tAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTCGCCCTGCAGCt < 1:763270/213‑1 (MQ=255) cGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCa < 1:892176/137‑1 (MQ=255) cGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCa > 2:892173/1‑137 (MQ=255) gggTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGCTGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCAGTCTTc > 2:678820/1‑168 (MQ=255) gggTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTCGCCCTGCAGCTGATAGGCCTTACCCAGCAGCTTCCAtgct < 1:557640/235‑1 (MQ=255) ggTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTCGCCCTGc > 2:118509/1‑201 (MQ=255) gTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTc < 1:225698/193‑1 (MQ=255) gTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTc < 2:466968/193‑1 (MQ=255) gTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTc > 2:225696/1‑193 (MQ=255) ttACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTCGCCCTGCGGCTGAGAGGCCTTACCcagc > 1:226631/1‑219 (MQ=255) ttACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTCGCCCTGCAGCTGATAGGCCTTACCCagcagc > 2:648500/1‑222 (MQ=255) aCCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTgcg > 2:86378/1‑174 (MQ=255) aTGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTCGCCCTGCAGCTGATAGGCCTTACCCAGCAGCTTCCATGCTGCCGAGTATTTCgg < 1:545614/235‑1 (MQ=255) aTGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTCGCCCTGCAGCTGATAGGCCTTACCCAGCAGCTTCCATGCTGCCGAGTATTTCgg < 1:704158/235‑1 (MQ=255) cGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTCTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCGCGCTTTGCTCGCCTCCGCCTGCTCGCCCTGCAGCTGATAGGCCTTACCCAGCAGCTTCCATGCTGCCGAGTATTTCgg < 2:503703/232‑1 (MQ=255) aTGTTGGATTCACCGACGTATTCGCGGGTAACCCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCtt < 2:306425/100‑1 (MQ=255) tGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTCGCCCTGCAGCt > 2:317468/1‑183 (MQ=255) tGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTCGCCCTGCAGCt < 1:317471/183‑1 (MQ=255) gATTCACCGCCGTATTCGCGGGTAACCCCGCTCCTACAGGGCCCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTCGCCCTGCAGCTGATAGGCCTTAccc < 2:110879/195‑1 (MQ=255) gATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTCGcc < 1:335461/173‑1 (MQ=255) gATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTCGcc > 2:335458/1‑173 (MQ=255) gACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTCGCCCTGCAGCTGATAGGCCTTAcc > 1:614556/1‑186 (MQ=255) gACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTCGCCCTGCAGCTGATAGGCCTTACCCAGCAGCTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTAcaca > 1:333755/1‑231 (MQ=255) gACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTCGCCCTGCAGCTGATAGGCCTTACCCAGCAGCTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGt > 1:408530/1‑235 (MQ=255) aTTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTGTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTCGCCCTGc > 2:814641/1‑164 (MQ=255) aaaCCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTg > 1:668516/1‑99 (MQ=255) tcccGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTCGCCCTGCAGCTGATAGGCCTTACCCAGCAGCTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTacac < 1:43432/212‑1 (MQ=255) ccGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTCGCCCTGCAGCTGATAGGCCTTACCCAGCAGCTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCtt < 1:499619/235‑1 (MQ=255) | TGCGCCGGAAGAGATGTACAGCACCGATGTGAATGTGTCGGCGGCGGCGATGAGCAAGGTGGTCGAACGCTATGTGCGCGAGTACCCCAGCCAGTACATGTGGAGCATGAAGCGCTTCAAGAAGCGCCCGGCTGGCGAGCCGCGCTGGTATTAAGCCATTCACCGTCCTCTGTAGGAACGGGTTTACCCGCGAATGCGATGTTGGATTCACCGACGTATTCGCGGGTAAACCCGCTCCTACAGGGACCGCGTCAAGCCGATGTCTTGTTCAGCTTCTTGAGAAAAACGGTCATTTCTTTTTCGGCCTGCTTGTCACCATGGGCCTGCGCCGCCACGATCCCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTCGCCCTGCAGCTGATAGGCCTTACCCAGCAGCTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTT > AE015451/74259‑74724 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |