| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 4,022,806 | C→G | 14.0% | intergenic (‑501/+167) | PP_3547 ← / ← emrB | putative NAD(P)‑binding oxidoreductase with NAD(P)‑binding Rossmann‑fold domain/multidrug efflux transport system ‑ permease subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 4,022,806 | 0 | C | G | 14.0% | 129.9 / 14.7 | 43 | intergenic (‑501/+167) | PP_3547/emrB | putative NAD(P)‑binding oxidoreductase with NAD(P)‑binding Rossmann‑fold domain/multidrug efflux transport system ‑ permease subunit |
| Reads supporting (aligned to +/- strand): ref base C (24/13); new base G (3/3); total (27/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.94e-01 | |||||||||||
TCATTCACGCAGGTGGCGCATAGTCACCCTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTGCCTTGATTTTAGCTCAATGCCCCGCCGATGCCGGGCCCGCCTTAGCGGTAAAGGGCGGCTTGGCGAACCACACCAGCAG > AE015451/4022605‑4023038 | tcattcaCGCAGGTGGCGCATAGTCACCCTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCt > 1:406279/1‑236 (MQ=255) caCGCAGGTGGCGCATAGTCACCCTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCg > 1:492627/1‑218 (MQ=255) aGGTGGCGCATAGTCACCCTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCg > 1:838619/1‑213 (MQ=255) agcgcgacgcggtccaagcacggctttcaaacccagcgctcttgatctcgatcttgatcttgatctcgatcttgatcttgatcttgatcttgatcttgatcttgatcttgatcttgatcttgatcttgatcttcgcgacttcaggaggccgaacgcaggccttgcgaaggcaggtgacgggcatCCATGCCCGTCACCTCCCTTCGCAAGCCCTACGCTCGGCCTCCTGAAGTCg > 1:183839‑M2/185‑235 (MQ=255) cTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCtt > 2:717715/1‑185 (MQ=255) cTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCtt < 1:717718/185‑1 (MQ=255) ttACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCt > 1:636628/1‑235 (MQ=255) ggcggGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAg < 1:122869/186‑1 (MQ=255) ttcaaacccagcgctcttgatctcgatcttgatcttgatctcgatcttgatcttgatcttgatcttgatcttgatcttgatcttgatcttgatcttgatcttgatcttcgcgacttcaggaggccgaacgcaggccttgcgaaggcaggtgacgggcatGGATGCCCGTCACCTGCCTTCGCaa > 2:645188‑M2/160‑184 (MQ=255) ttcaaacccagcgctcttgatctcgatcttgatcttgatctcgatcttgatcttgatcttgatcttgatcttgatcttgatcttgatcttgatcttgatcttgatcttcgcgacttcaggaggccgaacgcaggccttgcgaaggcaggtgacgggcatGGATGCCCGTCACCTGCCTTCGCaa < 1:645191‑M2/25‑1 (MQ=255) ggTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCtt < 2:651554/176‑1 (MQ=255) cttgatctcgatcttgatcttgatcttgatcttgatcttgatcttgatcttgatcttgatcttgatcttgatcttcgcgacttcaggaggccgaacgcaggccttgcgaaggcaggtgacgggcatCCATGCCCGTCACCTCCCTTCGCAAGCCCTACGCTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGAACTATCGCGCGCTTAGAAgcaaca > 1:197625‑M2/127‑216 (MQ=255) aCGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGcgaa > 2:451763/1‑170 (MQ=11) aCGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGaa > 2:634312/1‑164 (MQ=40) cTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGaa > 2:387240/1‑214 (MQ=255) ccatcatcCGGCCCCTGCGCTGCGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAagcgagagcgagagcgagagcgaga < 1:649794/233‑1 (MQ=255) ccatcatcCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCaa < 1:339956/130‑1 (MQ=18) cTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCCAGCCCTGCGCTCGGCCTCCTGAAGGCGCAATCCGCGGCGCCTg > 2:88047/1‑155 (MQ=255) cttgatcttgatcttgatcttgatcttgatcttcgcgacttcaggaggccgaacgcaggccttgcggaggcaggtgacgggcgtCCATGCCCGTCACCTCCCTCCGCACGCCCTGCGCGCGACCTCCTGAAGTc > 1:431932‑M2/85‑134 (MQ=255) ggggTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGaa > 2:757456/1‑165 (MQ=255) cccTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTGc < 2:926090/228‑1 (MQ=255) atcttgatcttgatcttgatcttcgcgacttcaggaggccgaacgcaggccttgcggaggcaggtgacgggcatCCAGGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGaa < 2:649037‑M2/86‑1 (MQ=255) cGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTGCCTTGATTTTAg > 1:907156/1‑235 (MQ=255) cGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTGCCTTGATTTTAg > 1:848866/1‑235 (MQ=255) gatcttgatcttgatcttcgcgacttcaggaggccgaacgcaggccttgcggaggcaggtgacgggcatCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACaa < 2:383571‑M2/86‑1 (MQ=255) ccGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTGCCTTGATTTTAGCTCAATGCCCCGCCGAt > 1:666174/1‑236 (MQ=255) ttcgcgacttcaggaggccgaacgcaggccttgcggaggcaggtgacgggcatGGATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTGCCTTGATTTTAGCTCAATGCCccgccg < 2:749465‑M2/181‑1 (MQ=255) gacttcaggaggccgaacgcaggccttgcggaggcaggtgacgggcatCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCg > 2:743018‑M2/49‑194 (MQ=255) gaCCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTGCCTTGATTTTAGCTCAATGCCCCGCCGATGCCggg < 1:365217/235‑1 (MQ=255) ccGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTGCCTTGATTTTAGCTCACTGCCCCGCCGTTGCCGGGcc < 2:492624/235‑1 (MQ=255) gcTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTGCCTTGATTTTAGATCAATGCCCCGCCGATGCCGGGCCCGCCt > 1:564366/1‑236 (MQ=255) gaggccgaacgcaggccttgcggaggcaggtgacgggcatGGATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTGCCTTGATTTTAGCTCAATGCCCCGCCGATGcc < 2:434872‑M2/186‑1 (MQ=255) cgaaGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATGGATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCCTAGACGCAAAATCAAAAGCCGAagc > 2:38635/4‑145 (MQ=17) aGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTGCCTTGATTTTAGCTCAATGCCCCGCCGATGCCGGGCCCGCCTTAGCg < 1:432682/236‑1 (MQ=255) gCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAagcgagagcgagagcgagagcgagagcgagagcatcgg > 2:433908/1‑169 (MQ=255) gCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTGCCTTGATTTTAGCTCAATGCCCCGCCGATGCCGGGCCCGCCTTAGCg > 2:432679/1‑234 (MQ=255) gcgcagggcttgcgaagggaggtgacgggcatCCATGCCCGTCCCCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAg > 2:867478‑M2/33‑131 (MQ=255) gcgcTTGACGGGCATGGATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTGCCTTGATTTTAGCTCAATGCCCCGCCGATGCCGGGCCCGCCTTAGCGGTAAAGGGCGGCTTGGCGa > 1:862825/1‑235 (MQ=255) cgcTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGCGCGAGAGCGAGAGCAt > 2:291704/1‑158 (MQ=255) gcTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTGCCTTGATTTTAGCTCAATGCCCCGCCGATGCCGGGCCCGCCTTAGCGGTAAAGGGCGGCTTGGCGacc > 1:167875/1‑233 (MQ=255) cTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATc < 1:36559/86‑1 (MQ=255) cTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATc > 2:36559/1‑86 (MQ=255) atCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTGCCTTGATTTTAGCTCAATGCCCCGCCGATGCCGGGCCCGCCTTAGCGGTAAAGGGCGGCTTGGCGAACCACACcagcag < 1:828702‑M2/233‑1 (MQ=255) | TCATTCACGCAGGTGGCGCATAGTCACCCTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTGCCTTGATTTTAGCTCAATGCCCCGCCGATGCCGGGCCCGCCTTAGCGGTAAAGGGCGGCTTGGCGAACCACACCAGCAG > AE015451/4022605‑4023038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |