Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	4,475,617	A→G	8.5%	G106G (GGT→GGC)	PP_3965 ←	transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	4,475,617	0	A	G	8.5%	163.0 / 4.9	47	G106G (GGT→GGC)	PP_3965	transposase
Reads supporting (aligned to +/- strand):  ref base A (17/26);  new base G (2/2);  total (19/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.49e-02

CTGTTCGATGAGGATTTCATCGTTCTGAATCTTCCTGCTCATCGCCTCGACCTGGGATTGCAATTGCAACGCCTGTGCCGCCAAGGCACGAAGCTGTTCCGGGGTCATCTGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGCTGTCGAGTTCGACCTCGCAGCCGTGGCGAATGCCGGGCCAGTGGAACTTGCCTTGGTTCAGTCGACGCGCTGCCAGCCAGACACCGACGCCATCGTGCACCAGCACTTTCATCCGGTTAGCCCGGCGATTGGCGAACAGATAAGCGCAGTGCGGCT  >  AE015451/4475401‑4475831                                                                                                                                                                                                                         |                                                                                                                                                                                                                       cTGTTCGATGAGGATTTCATCGTTCTGAATCTTCCTGCTCATCGCCTCGACCTGGGATTGCAATTGCAACGCCTGTGCCGCCAAGGCACGAAGCTGTTCCGGGGTCATCTGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGa                                                                                                                                                                                                           <  2:587541/230‑1 (MQ=255)  tGTTCGATGAGGATTTCATCGTTCTGAATCTTCCTGCTCATCGCCTCGACCTGGGATTGCAATTGCAACGCCTGTGCCGCCAAGGCACGAAGCTGTTCCGGGGTCATCTGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCt                                                                                                                                                                                                     <  1:925664/235‑1 (MQ=255)           aGGATTTCATCGTTCTGAATCTTCCTGCTCATCGCCTCGACCTGGGATTGCAATTGCAACGCCTGTGCCGCCAAGGCACGAAGCTGTTCCGGGGTCATCTGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAg                                                                                                                                                                                           <  1:45216/236‑1 (MQ=255)                  cATCGTTCTGAATCTTCCTGCTCATCGCCTCGACCTGGGATTGCAATTGCAACGCCTGTGCCGCCAAGGCACGAAGCTGTTCCGGGGTCATCTGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGc                                                                                                                                                                                    <  1:904666/236‑1 (MQ=255)                             aTCTTCCTGCTCATCGCCTCGACCTGGGATTGCAATTGCAACGCCTGTGCCGCCAAGGCACGAAGCTGTTCCGGGGTCATCTGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACgcgc                                                                                                                                                                                                      <  1:46471/207‑1 (MQ=255)                                   cTGCTCATCGCCTCGACCTGGGATTGCAATTGCAACGCCTGTGCCGCCAAGGCACGAAGCTGTTCCGGGGTCATCTGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCAc                                                                                                                                                                                                              <  2:21414/193‑1 (MQ=255)                                    tGCTCATCGCCTCGACCTGGGATTGCAATTGCAACGCCTGTGCCGCCAAGGCACGAAGCTGTTCCGGGGTCATCTGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAgttgt                                                                                                                                                                  <  1:504678/236‑1 (MQ=255)                                          tCGCCTCGACCTGGGATTGCAATTGCAACGCCTGTGCCGCCAAGGCACGAAGCTGTTCCGGGGTCATCTGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGc                                                                                                                                                              <  1:758970/234‑1 (MQ=255)                                            gCCTCGACCTGGGATTGCAATTGCAACGCCTGTGCCGCCAAGGCACGAAGCTGTTCCGGGGTCATCTGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCAc                                                                                                                                                                                                <  2:556573/198‑1 (MQ=255)                                            gCCTCGACCTGGGATTGCAATTGCAACGCCTGTGCCGCCAAGGCACGAAGCTGTTCCGGGGTCATCTGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGATTACAGCATGCTGATCACGCCACCCGCGCCGACTCGCt                                                                                                                                                                                                     <  2:745361/193‑1 (MQ=11)                                            gCCTCGACCTGGGATTGCAATTGCAACGCCTGTGCCGCCAAGGCACGAAGCTGTTCCGGGGTCATCTGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGATTACAGCATGCTGATCACGCCACCCGCGCCGACTCGCt                                                                                                                                                                                                     >  1:745364/1‑193 (MQ=11)                                                        gATTGCAATTGCAACGCCTGTGCCGCCAAGGCACGAAGCTGTTCCGGGGTCATCTGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCAc                                                                                                                                                                                                <  2:858882/186‑1 (MQ=255)                                                               attgcaaCGCCGGTGCCGCCAAGGCACGAAGCTGTTCCGGGGTCATCTGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGc                                                                                                                                                                            <  2:22607/199‑1 (MQ=255)                                                                ttgcaaCGCCTGTGCCGCCAAGGCACGAAGCTGTTCCGGGGTCATCTGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTGATCACGCCACCCGCGCCGACTCGCt                                                                                                                                                                                                     <  2:812989/173‑1 (MQ=11)                                                                ttgcaaCGCCTGTGCCGCCAAGGCACGAAGCTGTTCCGGGGTCATCTGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTGATCACGCCACCCGCGCCGACTCGCt                                                                                                                                                                                                     >  1:812992/1‑173 (MQ=11)                                                                 tgcaaCGCCTGTGCCGCCAAGGCACGAAGCTGTTCCGGGGTCATCTGGTCGAGATTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGACCAGGCGCGATGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGCTGTCGAGTTCGACCTCGCAGCCg                                                                                                                                       >  2:382549/1‑234 (MQ=255)                                                                    aaCGCCTGTGCCGCCAAGGCACGAAGCTGTTCCGGGGTCATCTGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGCTGTCGAGt                                                                                                                                                      <  1:430110/216‑1 (MQ=255)                                                                    aaCGCCTGTGCCGCCAAGGCACGAAGCTGTTCCGGGGTCATCTGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGCTGTCGAGt                                                                                                                                                      >  2:430107/1‑216 (MQ=255)                                                                       gCCTGTGCCGCCAAGGCACGAAGCTGTTCCGGGGTCATCTGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACg                                                                                                                                                                                               <  2:741256/172‑1 (MQ=255)                                                                          tgtgCCGCCAAGGCACGAAGCTGTTCCGGGGTCATCTGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGGTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGCCAGGccccg                                                                                                                                                                                     >  2:179143/1‑177 (MQ=255)                                                                           gtgCCGCCAAGGCACGAAGCTGTTCCGGGGTCATCTGGTCGAGATTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGACCAGGCGCGATGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGCTGTCGAGTTCGACCTCGCAGCCGTGGCGAATGcc                                                                                                                            <  1:382552/235‑1 (MQ=255)                                                                              ccgccAAGGCACGAAGCTGTTCCGGGGTCATCTGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGCTGTCGAGTTCGACCTCGCAGCCGt                                                                                                                                      >  1:459653/1‑222 (MQ=255)                                                                                     ggCACGAAGCTGTTCCGGGGTCATCTGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCg                                                                                                                                                                                                            <  2:389670/145‑1 (MQ=255)                                                                                                    cGGGGTCATCTGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGc                                                                                                                                                              <  1:552577/176‑1 (MQ=255)                                                                                                    cGGGGTCATCTGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGCTGTCGAGTTCGACCTCGCAGCCGTGGCGAATGCCGGGCCAGTGGAACTTGCCTTGGTTCa                                                                                                  >  1:740135/1‑236 (MQ=255)                                                                                                              tGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGCTGTCGAGTTCGACCTCGCAGCCGTGGCGAATGCCGGGc                                                                                                                        <  1:654322/204‑1 (MQ=255)                                                                                                              tGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGCTGTCGAGTTCGACCTCGCAGCCGTGGCGAATGCCGGGc                                                                                                                        >  2:654319/1‑204 (MQ=255)                                                                                                              tGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGCTGTCGAGTTCGACCTCGCAGCCGTGGCGAATGCCGGGCCAGTGGAACTTGCCTTg                                                                                                       >  2:756772/1‑221 (MQ=255)                                                                                                              tGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGCTGTCGAGTTCGACCTCGCAGCCGTGGCGAATGCCGGGCCAGTGGAACTTGCCTTGGTTCAGTCGa                                                                                             >  2:327344/1‑231 (MQ=255)                                                                                                              tGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGCTGTCGAGTTCGACCTCGCAGCCGTGGCGAATGCCGGGCCAGTGGAACTTGCCTTGGTTCAGTCGAcg                                                                                           >  2:792757/1‑233 (MQ=255)                                                                                                                         gggCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCa                                                                                                                                                                                >  2:108530/1‑137 (MQ=255)                                                                                                                              agaAAGTCATGCCGCCGATTGTGCCAGACCAGGCGCGATGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCGCCAACGCCTGGAGTTGTTCGCTGTCGAGTTCGACCTc                                                                                                                                              <  1:559341/166‑1 (MQ=255)                                                                                                                              agaAAGTCATGCCGCCGATTGTGCCAGACCAGGCGCGATGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGCTGTCGAGTTCGACCTc                                                                                                                                              >  2:559338/1‑166 (MQ=255)                                                                                                                                 aaGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGCTGTCGAGTTCGACCTCGCAGCCGTGGCGAATGCCGGGCCAGTGGAACTTGCCTTGGTTCAGTCGACGCGCTGCCAGCCAGACACCGACg                                                                     <  1:786641/236‑1 (MQ=255)                                                                                                                                    tCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGCTGTCGAGTTCGACCTCGCAGCCGTGGCGAATGCCGGGCCAGTGGAACTTGCCTTGGTTCAGTCGACGCGCTGCCAGCCAGACACCGACg                                                                     <  2:365808/233‑1 (MQ=255)                                                                                                                                            ccgATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGCTGTCGAGTTCGACCTCGCAGCCGTGGCGAATGCCGGGCCAGTGGAACTTGCCTTGGTTCAGTCGACGCGCTGCCAGCCAGACACCGACGCCATCGTGCAc                                                          <  1:792760/236‑1 (MQ=255)                                                                                                                                               aTTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCa                                                                                                                                                                                   >  2:381065/1‑112 (MQ=255)                                                                                                                                               aTTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCa                                                                                                                                                                                   <  1:381068/112‑1 (MQ=255)                                                                                                                                                     ccAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGCTGTCGAGTTCGACCTCGCAGCCGTGGCGAATGCCGGGCCAGTGGAACTTGCCTTGGTTCAGTCGACGCGCTGCCAGCCAGACACCGACGCCATCGTGCACCAGc                                                      <  2:548330/231‑1 (MQ=255)                                                                                                                                                                              aGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGCTGTCGAGTTCGACCTCGCAGCCGTGGCGAATGCCGGGCCAGTGGAACTTGCCTTGGTTCAGTCGACGCGCTGCCAGCCAGACACCGACGCCATCGTGCACCAGCACTTTCATCCGGTTAGCCCGGCGATTGGc                         <  1:756775/235‑1 (MQ=255)                                                                                                                                                                               gTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGCTGTCGAGTTCGACCTCGCAGCCGTGGCGAATGCCGGGCCAGTGGAACTTGCCTTGGTTCAGTCGACGCGCTGCCAGCCAGACACCg                                                                        <  2:924358/187‑1 (MQ=255)                                                                                                                                                                                        ccAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCGGCCACGGCAGGCCCAGCACCAACGCACGGAgttgtt                                                                                                                                                                 >  2:176565/1‑89 (MQ=255)                                                                                                                                                                                          aaaTGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGCTGTCGAGTTCGACCTCGCAGCCGTGGCGAATGCCGGGCCAGTGGAACTTGCCTTGGTTCAGTCGACGCGCTGCCAGCCAGACACCGACGCCa                                                                  >  2:925281/1‑182 (MQ=255)                                                                                                                                                                                          aaaTGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGCTGTCGAGTTCGACCTCGCAGCCGTGGCGAATGCCGGGCCAGTGGAACTTGCCTTGGTTCAGTCGACGCGCTGCCAGCCAGACACCGACGCCa                                                                  <  1:925284/182‑1 (MQ=255)                                                                                                                                                                                                    cGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGCTGTCGAGTTCGACCTCGCAGCCGTGGCGAATGCCGGGCCAGTGGAACTTGCCTTGGTTCAGTCGACGCGCTGCCAGCCAGACACCGa                                                                       >  1:131057/1‑167 (MQ=255)                                                                                                                                                                                                                 cTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGCTGTCGAGTTCGACCTCGCCGCCGTGGCGAATGCCGGGCCAGTGGAACTTGCCTTGGTTCAGTCgg                                                                                             >  2:216947/1‑131 (MQ=255)                                                                                                                                                                                                                 cTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGCTGTCGAGTTCGACCTCGCAGCCGTGGCGAATGCCGGGCCAGTGGAACTTGCCGTGGtt                                                                                                    >  2:299177/1‑125 (MQ=255)                                                                                                                                                                                                                       ccacCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGCTGTCGAGTTCGACCTCGCAGCCGTGGCGAATGCCGGGCCAGTGGAACTTGCCTTGGTTCAGTCGACGCGCTGCCAGCCAGACACCGACGCCATCGTGCACCAGCACTTTCATCCGGTTAGCCCGGCGATTGGCGAACAGATAAGCGCAGTGCGGCt  <  1:174267/216‑1 (MQ=255)                                                                                                                                                                                                                         |                                                                                                                                                                                                                       CTGTTCGATGAGGATTTCATCGTTCTGAATCTTCCTGCTCATCGCCTCGACCTGGGATTGCAATTGCAACGCCTGTGCCGCCAAGGCACGAAGCTGTTCCGGGGTCATCTGGTCGAGGTTGGGCGAGAAAGTCATGCCGCCGATTGTGCCAGAGCAGGCGCGGGGCGACGACAAGTAGACCGGCCAAATGGCCGGCGTTTACAGCATGCTAATCACACCGCCTGCACCGACGCGCTGCCACGGCAGGCCCAGCACCAACGCCTGGAGTTGTTCGCTGTCGAGTTCGACCTCGCAGCCGTGGCGAATGCCGGGCCAGTGGAACTTGCCTTGGTTCAGTCGACGCGCTGCCAGCCAGACACCGACGCCATCGTGCACCAGCACTTTCATCCGGTTAGCCCGGCGATTGGCGAACAGATAAGCGCAGTGCGGCT  >  AE015451/4475401‑4475831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑