| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 4,964,454 | C→T | 100% | D302N (GAC→AAC) | fleQ ← | Transcriptional regulator FleQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 4,964,454 | 0 | C | T | 94.3% | 114.2 / ‑1.3 | 35 | D302N (GAC→AAC) | fleQ | Transcriptional regulator FleQ |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (20/13); total (20/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.33e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGCGCTCGACCAGGTTGGCCAGCTCGCGTACGTTGCCCGGCCAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCG > AE015451/4964244‑4964665 | tGCGCTCGACCAGGTTGGCCAGCTCGCGTACGTTGCCCGGCCAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGAt > 1:429069/1‑235 (MQ=255) ggCCAGCTCGCGTACGTTGCCCGGCCAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCg > 1:863715/1‑217 (MQ=255) aGCTCGCGTACGTTGCCCGGCCAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTtgtg < 1:667112/236‑1 (MQ=255) cTCGCGTACGTTGCCCGGCCAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTtgtg < 2:419813/234‑1 (MQ=255) ccgcgTACGTTGCCCGGCCAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTcgc < 1:219408/192‑1 (MQ=255) ccGGCCAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCgatgat > 1:485556/1‑235 (MQ=255) gTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTc > 1:820929/1‑234 (MQ=255) gCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGCGCCGTCCTCGATCATGGTTTCGa > 1:193557/1‑189 (MQ=255) tGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGc < 1:16893/222‑1 (MQ=255) tCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCt > 1:759339/1‑235 (MQ=255) aGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTAccc < 1:340729/236‑1 (MQ=255) gCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCa > 1:820202/1‑235 (MQ=255) ggAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGtt > 2:390760/1‑169 (MQ=255) aGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCAcgc < 1:69840/228‑1 (MQ=255) gCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAg < 2:255423/235‑1 (MQ=255) ggAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGACGGTGCCGTCCTCGATCCTGGTTTCGCGGTTCTtgt > 2:70087/1‑167 (MQ=255) gcgcTACTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACg < 1:437148/184‑1 (MQ=255) cgcTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTgcag < 1:217495/236‑1 (MQ=255) ttCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGCTCATGGTTTCGA‑GTTCTTGGGCGTCGCAGCGATGATGCGCACGTCg > 2:96989/1‑182 (MQ=255) ttCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGcac > 1:57073/1‑236 (MQ=255) tcGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCacac < 1:553391/235‑1 (MQ=255) gTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGGACg > 1:265350/1‑219 (MQ=255) gTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCACTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGGTTTcgg > 2:815113/1‑112 (MQ=255) gcgAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTAc > 2:217842/1‑189 (MQ=255) gcgAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGCAGGTACGCt > 2:141402/1‑210 (MQ=255) aaTCAGCTCGTTCATCAGCAACGGGATGTCTTCCCCCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGAcc < 1:670077/232‑1 (MQ=255) cgcGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGt < 2:72677/133‑1 (MQ=255) cAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCACCGGCATGTCGc > 1:48942/1‑236 (MQ=255) ggATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTCGTACAGGCTTTCGCGGAAGGTGCCGTCCTCGTTCATGGTTTCGAGGTTCTTGTGCGGCGCAGCGATGATGCGCACGCCGATGCttc > 2:316465/1‑146 (MQ=255) cACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTa > 1:727196/1‑236 (MQ=255) gcggcgCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAg < 2:759336/220‑1 (MQ=255) aaCACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGGTGGCCAGGTCGAAACGccc > 1:270724/1‑236 (MQ=255) acacGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCg < 1:735668/236‑1 (MQ=255) ggTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCtt < 1:869224/148‑1 (MQ=255) ggTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCtt > 2:869221/1‑148 (MQ=255) | TGCGCTCGACCAGGTTGGCCAGCTCGCGTACGTTGCCCGGCCAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCG > AE015451/4964244‑4964665 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |