Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	196,351	C→G	21.3%	T619T (ACC→ACG)	PP_0168 →	putative surface adhesion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	196,351	0	C	G	21.3%	100.6 / 27.1	56	T619T (ACC→ACG)	PP_0168	putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base C (23/21);  new base G (9/3);  total (32/24)
Fisher's exact test for biased strand distribution p-value = 2.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

GGTCAGCGTCACCATCGAGAACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA  >  AE015451/196132‑196570                                                                                                                                                                                                                            |                                                                                                                                                                                                                            ggTTAGCGTCACCATCGAGAACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                                                                                             <  1:546156/236‑1 (MQ=255) ggTCAGCGTCACCATCGAGAACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                                                                                             <  1:938279/236‑1 (MQ=255)                         cACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCa                                                                                                                                                                                                                            <  2:357079/197‑1 (MQ=40)                         cACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCa                                                                                                                                                                                                                            >  1:357082/1‑197 (MQ=255)                               cGGTAATTTCGACCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                                                                                             <  2:354266/206‑1 (MQ=38)                                           gCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGc                                                                                                                                                                                                           >  1:65763/1‑196 (MQ=38)                                           gCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGa                                                                                                                                                                      <  2:563327/233‑1 (MQ=21)                                                     ccGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAt                                                                                                                                                                    <  2:695321/225‑1 (MQ=14)                                                          cccGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGa                                                                                                                                                                                       >  1:435113/1‑201 (MQ=14)                                                                    gcccAGACCACCATCAACGACCCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGa                                                                                                                                                                      <  2:360745/205‑1 (MQ=9)                                                                        aGACCACGATCACCGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                                                                                             <  2:124971/165‑1 (MQ=18)                                                                          aCCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGtt                                                                                                                                                 <  1:310645/223‑1 (MQ=21)                                                                                 tCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCaaga                                                                                                                                                                                                       >  1:523256/1‑160 (MQ=39)                                                                                                acacCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGAcc                                                                                                                                                                             >  1:604269/1‑173 (MQ=21)                                                                                                          cgcgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                                                                                             <  2:348464/131‑1 (MQ=32)                                                                                                          cgcgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAAttt                                                                                                      >  1:606715/1‑234 (MQ=9)                                                                                                          cgcgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTc                                                                                                     >  1:809718/1‑235 (MQ=12)                                                                                                          cgcgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                                                                                             >  1:348467/1‑131 (MQ=17)                                                                                                              aCCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCAca                                                                                                                 >  1:925372/1‑218 (MQ=17)                                                                                                                               gTCGGACACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTc                                                                                                                                                                                         >  2:778978/1‑130 (MQ=255)                                                                                                                                 cGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAGACCATCACCgg                                                                                                                                                                                                                     >  2:78331/1‑99 (MQ=11)                                                                                                                                           aGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGAc                                                                                          <  2:809715/213‑1 (MQ=2)                                                                                                                                               ggagTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAAcc                                                                                                                                                                                                                             <  1:614432/75‑1 (MQ=21)                                                                                                                                                 cgTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                                                                                             <  1:467262/92‑1 (MQ=33)                                                                                                                                                 cgTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                                                                                             >  2:467259/1‑92 (MQ=16)                                                                                                                                                    gATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAGACCATCACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGActgttc                                                                                                                                                            >  1:819404/1‑133 (MQ=11)                                                                                                                                                        aCTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGAc                                                                                                                                             >  1:460395/1‑149 (MQ=34)                                                                                                                                                          ttACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAAt                                                                                                        <  1:855368/184‑1 (MQ=2)                                                                                                                                                          gtACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCg                                                                          <  1:754575/213‑1 (MQ=255)                                                                                                                                                                cGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGTAAAAc                                                                                                                                                                                                      >  1:4065/1‑84 (MQ=18)                                                                                                                                                                 gTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACcg                                                                                                                >  2:938975/1‑169 (MQ=37)                                                                                                                                                                     cccTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGc                                                                                                                                                                                            <  2:682897/89‑1 (MQ=35)                                                                                                                                                                     cccTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGc                                                                                                                                                                                            >  1:682900/1‑89 (MQ=33)                                                                                                                                                                     cccTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGt                                                                                                                                                                                          >  1:170910/1‑91 (MQ=33)                                                                                                                                                                     cccTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGt                                                                                                                                                                                          <  2:170909/91‑1 (MQ=33)                                                                                                                                                                     cccTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGc                                          >  1:724504/1‑235 (MQ=39)                                                                                                                                                                     cccTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGAc                                                                                                                                                                 <  2:56664/116‑1 (MQ=11)                                                                                                                                                                     cccTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGAc                                                                                                                                                                 >  1:56664/1‑116 (MQ=11)                                                                                                                                                                       cTGAGCAATCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGc                                                                                                                                                                                            <  2:407059/87‑1 (MQ=255)                                                                                                                                                                       cTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCg                                                                                                                                                                                           <  2:674631/88‑1 (MQ=255)                                                                                                                                                                       cTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCg                                                                                                                                                                                           >  1:674634/1‑88 (MQ=255)                                                                                                                                                                       cTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCg                                                                                                                                                                       >  2:552513/1‑108 (MQ=11)                                                                                                                                                                             aaTCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGAcaccact                                      <  1:752166/231‑2 (MQ=12)                                                                                                                                                                             aaTCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGAcaccact                                      >  2:752163/1‑230 (MQ=16)                                                                                                                                                                             aaTCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGGAACTGACCCCgc                                                                                     >  1:96712/1‑183 (MQ=37)                                                                                                                                                                                 cTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCCGCAAAACCCAGGGCAg                                                                                                                                                                                             >  1:669217/1‑76 (MQ=21)                                                                                                                                                                                  tGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGcca                                                                                                                                                                      >  1:721847/1‑96 (MQ=255)                                                                                                                                                                                  tGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGCaaaa                                                                                                                                                                                                       >  2:930293/1‑65 (MQ=18)                                                                                                                                                                                      cAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTc                                                   <  1:15724/209‑1 (MQ=255)                                                                                                                                                                                       aGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCCTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGc                                                                                                                     >  1:716361/1‑142 (MQ=255)                                                                                                                                                                                                tGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGAc                                                                                                                                                                              <  2:669214/76‑1 (MQ=34)                                                                                                                                                                                                 gaccgTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCgg                                                                                                                                                                         >  1:531756/5‑80 (MQ=1)                                                                                                                                                                                                          ccTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCg                                                                                                                                                                       >  1:928090/1‑73 (MQ=34)                                                                                                                                                                                                              tCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGaa  <  2:724501/234‑1 (MQ=37)                                                                                                                                                                                                               ccAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGc                                                                                                                                                                                               >  1:368666/1‑44 (MQ=38)                                                                                                                                                                                                                cAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGa                                                                   <  2:53099/167‑1 (MQ=38)                                                                                                                                                                                                                      ccAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAg                                                                                                                                                                                             >  1:537910/1‑39 (MQ=255)                                                                                                                                                                                                                           ccATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACcggcgg                                                                                                            >  2:178008/1‑115 (MQ=39)                                                                                                                                                                                                                            |                                                                                                                                                                                                                            GGTCAGCGTCACCATCGAGAACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA  >  AE015451/196132‑196570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑