Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	152,157	A→T	13.8%	intergenic (‑51/+43)	PP_0143 ← / ← PP_0144	conserved membrane protein of unknown function/Metalloprotease, insulinase family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	152,157	0	A	T	13.8%	81.5 / 6.6	29	intergenic (‑51/+43)	PP_0143/PP_0144	conserved membrane protein of unknown function/Metalloprotease, insulinase family
Reads supporting (aligned to +/- strand):  ref base A (4/21);  new base T (2/2);  total (6/23)
Fisher's exact test for biased strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.72e-01

CAGCTGACCAGCATGAGCACGGCGACCCAGCTGGCCAGGCCAGCCTGGCGGTCGACCAGGATGAAGCTGCCGATACCGGAGAGGAAACCGCCGAGCGCAATGATGCCCGGGTAGCGCTTGAGTAGTTCGATGATGCGCTGGACGATATGGGGGATTTGGGGCATTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGAGCAAGGCCATCAACCCGTAGAGCTCGTCATAGCCTAGCAACGGCTTTTCGATGCGCACATAGCCATCTTCCTTGAGCAGATCCTTCAATGCC  >  AE015451/151943‑152377                                                                                                                                                                                                                       |                                                                                                                                                                                                                             cAGCTGACCAGCATGAGCACGGCGACCCAGCTGGCCAGGCCAGCCTGGCGGTCGACCAGGATGAAGCTGCCGATACCGGAGAGGAAACCGCCGAGCGCAATGATGCCCGGGTAGCGCTTGAGTAGTTCGATGATGCGCTGGACGATATGGGGGATTTGGGGCATTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCgg                                                                                                                                                                                                            >  2:864931/1‑233 (MQ=255)     ggACAAGCATGAGCACGGCGACCCAGCTGGCCAGGCCAGCCTGGCGGTCGACCAGGATGAGGCTGCCGATACCGGAGAGGAAACCGCCGAGCGCAATGATGCCCGGGTAGCGCTTGAGTAGTTCGATGATGCGCTGGACGATATGGGGGATTTGGGGCATTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTc                                                                                                                                                                                                              <  2:717192/226‑1 (MQ=255)         cAGCATGAGCACGGCGACCCAGCTGGCCAGGCCAGCCTGGCGGTCGACCAGGATGAAGCTGCCGATACCGGAGAGGAAACCGCCGAGCGCAATGATGCCCGGGTAGCGCTTGAGTAGTTCGATGATGCGCTGGACGATATGGGGGATTTGGGGCATTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCaaaa                                                                                                                                                                                                 <  1:713334/236‑1 (MQ=255)                aGCACGGCGACCCAGCTGGCCAGGCCAGCCTGGCGGTCGACCAGGATGAAGCTGCCGATACCGGAGAGGAAACCGCCGAGCGCAATGATGCCCGGGTAGCGCTTGAGTAGTTCGATGATGCGCTGGACGATATGGGGGATTTGGGGCATTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAg                                                                                                                                                                                                                  <  2:521811/212‑1 (MQ=255)                       ggACCCAGCTGGCCAGGCCAGCCTGGCGGTCGACCAGGATGAAGCTGCCGATACCGGAGAGGAAACCGCCGAGCGCAATGATGCCCGGGTAGCGCTTGAGTAGTTCGATGATGCGCTGGACGATATGGGGGATTTGGGGCATTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGAtt                                                                                                                                                                                   <  1:718327/235‑1 (MQ=255)                                  gccaggccagCCTGGCGGTCGACCAGGATGAAGCTGCCGATACCGGAGAGGAAACCGCCGAGCGCAATGATGCCCGGGTAGCGCTTGAGTAGTTCGATGATGCGCTGGACGATATGGGGGATTTGGGGCATTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCcgc                                                                                                                                                                                                                       <  2:813850/189‑1 (MQ=255)                                                                   ttGCCGATCCCGGAGAGGAAACCGCCGAGCGCAAGGATGCCCGGGTAGCGCTTGAGTAGTTCGATGATGCGCTGGACGATATGGGGGATTTGGGGCATTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCg                                                                                                                                                                                                             <  2:313478/165‑1 (MQ=255)                                                                       cGATACCGGAGAGGAAACCGCCGAGCGCAATGATGCCCGGGTAGCGCTTGAGTAGTTCGATGATGCGCTGGACGATATGGGGGATTTGGGGCATTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCg                                                                                                                                                                              <  1:296490/193‑1 (MQ=255)                                                                         aTACCGGAGAGGAAACCGCCGAGCGCAATGATGCCCGGGTAGCGCTTGAGTAGTTCGATGATGCGCTGGACGATATGGGGGATTGGGGGCATTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACCAGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTa                                                                                                                                                                    >  2:515463/1‑201 (MQ=255)                                                                             cGGAGAGGAAACCGCCGAGCGCAATGATGCCCGGGTAGCGCTTGAGTAGTTCGATGATGCGCTGGACGATATGGGGGATTTGGGGCATTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCg                                                                                                                                                                              <  1:209076/187‑1 (MQ=255)                                                                                      caCCGCCGAGCGCAATGATGCCCGGGTAGCGCTTGAGTAGTTCGATGATGCGCTGGACGATATGGGGGATTTGGGGCATTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCg                                                                                                                                                                              <  2:34172/177‑1 (MQ=255)                                                                                      aaCCGCCGAGCGCAATGATGCCCGGGTAGCGCTTGAGTAGTTCGATGATGCGCTGGACGATATGGGGGATTTGGGGCATTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGaacg                                                                                                                                                                                                                  <  2:581645/142‑3 (MQ=255)                                                                                        ccgccgAGCGCAATGATGCCCGGGTAGCGCTTGAGTAGTTCGATGATGCGCTGGACGATATGGGGGATTTGGGGCATTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCgtg                                                                                                                                                                      <  1:187944/184‑1 (MQ=255)                                                                                                  aaaggatgCCCGGGTGGCGCGTGAGTAGTTCGATGATGCGCTGGACGATATGGGGGATTTGGGGCATTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCgtg                                                                                                                                                                      <  2:94644/170‑1 (MQ=255)                                                                                                                   cgcTTGAGTAGTTCGATGATGCGCTGGACGATATGGGGGATTTGGGGCATTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGAGCAAgg                                                                                        <  1:450294/235‑1 (MQ=255)                                                                                                                    gcTTGAGTAGTCCGATGATGCGCTGGACGATATGGGGGATTTGGGGCATTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCcgc                                                                                                                                                                                                                       <  1:635818/107‑1 (MQ=255)                                                                                                                      ttGAGTAGTTCGATGATGCGCTGGACGATATGGGGGATTTGGGGCATTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCTTGCCcgc                                                                                                                                                                                                                       >  2:806818/1‑105 (MQ=255)                                                                                                                                         gcTGGACGATATGGGGGATTTGGGGCATTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCTTGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAgg                                                                                                           <  1:468029/194‑1 (MQ=255)                                                                                                                                              gcGATATGGGGGATTTGGGGCATTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGAGCAAGGCCATCAACCCGTa                                                                           <  1:858671/220‑1 (MQ=255)                                                                                                                                                               gggCATTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGc                                                                                                                                                                          >  1:818870/1‑109 (MQ=255)                                                                                                                                                                     tGGGGGCTACGACAGAAAAGTGCGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCTCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGAGCAAGGCCATCAACCCGTa                                                                           <  2:532678/198‑1 (MQ=255)                                                                                                                                                                       ggggCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGc                                                                                                          <  2:832741/165‑1 (MQ=255)                                                                                                                                                                                 cAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCTTGCCCGCGAAAg                                                                                                                                                                                                                  >  1:523833/1‑51 (MQ=21)                                                                                                                                                                                 cAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCTTGCCCGCGAAAg                                                                                                                                                                                                                  <  2:523830/51‑1 (MQ=21)                                                                                                                                                                                            ggTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGAGCAAGGCCATCAACCCGTaga                                                                         <  1:34544/177‑1 (MQ=255)                                                                                                                                                                                                           ctctTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGAGCAAGGCCATCAACCCGTAGAGCTCGTCATAGCCTAGCAACGGCTTTTCGAt                                          <  1:41439/193‑1 (MQ=255)                                                                                                                                                                                                              ttCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGAGCAAGGCCATCAACCCGTAGAGCTCGTCATAGCCTAGCAACGGCTTTTCGATGCGCACATAGCCATCTTCCTTGAGCAGATCCTTCAATGcc  <  2:572096/230‑1 (MQ=255)                                                                                                                                                                                                                 gcgGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGAGCAAGGCCATCaa                                                                                 >  2:269668/1‑148 (MQ=255)                                                                                                                                                                                                                    ggCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGTTACCGCCGATTTACCGCTGCCGTGTAGCACTACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGATCAAGGCCATCAACCCGTAGAGCTCGTCATAGCCTAGCAACGGCTTTTCGATGCGCACATAGCCATCTTCCTTGAGCATATCCTTCa       <  1:515466/219‑1 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                                             CAGCTGACCAGCATGAGCACGGCGACCCAGCTGGCCAGGCCAGCCTGGCGGTCGACCAGGATGAAGCTGCCGATACCGGAGAGGAAACCGCCGAGCGCAATGATGCCCGGGTAGCGCTTGAGTAGTTCGATGATGCGCTGGACGATATGGGGGATTTGGGGCATTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGAGCAAGGCCATCAACCCGTAGAGCTCGTCATAGCCTAGCAACGGCTTTTCGATGCGCACATAGCCATCTTCCTTGAGCAGATCCTTCAATGCC  >  AE015451/151943‑152377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑