Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AE015451 197,551 C→G 6.2% T1019T (ACC→ACG)  PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451197,5510CG 6.2% 164.7 / 4.4 63T1019T (ACC→ACG) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base C (34/25);  new base G (2/2);  total (36/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.41e-01

TTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCAC  >  AE015451/197326‑197784
                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                         
ttCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTg                                                                                                                                                                                                                                 >  1:909404/1‑236 (MQ=37)
  cGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGtt                                                                                                                                                                                                                                  <  2:659013/233‑1 (MQ=37)
         gagacagaCCATCGAGAACGCCACCGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCa                                                                                                                                                                                                                        <  1:175542/229‑1 (MQ=25)
                                           ttCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                                                                                                           >  2:669769/1‑199 (MQ=34)
                                           ttCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                                                                                                           <  1:669772/199‑1 (MQ=37)
                                                                      ccGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGAc                                                                                                                                                           >  1:587191/1‑236 (MQ=33)
                                                                       cGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACt                                                                                                                                                          <  1:117679/236‑1 (MQ=9)
                                                                             aGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCggga                                                                                                                                                                                                                        >  1:742940/1‑166 (MQ=25)
                                                                                                 gTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGcg                                                                                                                                                                                                                            <  2:601458/144‑2 (MQ=17)
                                                                                                         caccacCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAt                                                                                                                                                                                  >  1:132839/1‑178 (MQ=35)
                                                                                                               cgcgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCca                                                                                                                                 >  1:621299/1‑221 (MQ=16)
                                                                                                               cgcgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTCGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAGCGCCACAGGCGGCaacgtc                                                                                                                   >  1:239045/1‑231 (MQ=255)
                                                                                                                  gACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGc                                                                                                               <  1:382638/236‑1 (MQ=17)
                                                                                                                   aCCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTc                                                                                                                                                                                                 >  2:612205/1‑153 (MQ=35)
                                                                                                                   aCCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                                                                                                           <  2:107513/127‑1 (MQ=17)
                                                                                                                    cccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAt                                                                                                                                                                                  <  2:917920/167‑1 (MQ=9)
                                                                                                                     ccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGAc                                                                                                                                                                               <  2:583636/169‑1 (MQ=35)
                                                                                                                     ccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGc                                                                                                                                                    <  2:587188/196‑1 (MQ=18)
                                                                                                                                        gTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTAACCCCGAATCCGAccc                                                                                          >  1:792957/1‑233 (MQ=2)
                                                                                                                                                        tGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGaatc                                                                                                >  1:351494/1‑211 (MQ=14)
                                                                                                                                                             aCCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGa                                                                                                                                                                                    <  2:777540/124‑1 (MQ=9)
                                                                                                                                                                    ccGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCTTTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGt                                                                                                                         >  1:520327/1‑176 (MQ=38)
                                                                                                                                                                     cGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTc                                                             >  1:575321/1‑235 (MQ=40)
                                                                                                                                                                      gTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAg                                                                                                                                                                                              <  2:453272/105‑1 (MQ=18)
                                                                                                                                                                       tGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGa                                                           >  1:629418/1‑235 (MQ=255)
                                                                                                                                                                        gacccTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                                                                                                           <  2:212157/71‑1 (MQ=17)
                                                                                                                                                                        gacccTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAg                                                                                                                                                                                              <  2:809282/100‑1 (MQ=17)
                                                                                                                                                                        gATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCCTGGTCCATATCCACACCTCcgcgcac                                                                                                                                                                                  >  1:632911/1‑112 (MQ=14)
                                                                                                                                                                          tCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAAcc                                                                                                                                                                                                                                           <  1:793626/56‑1 (MQ=40)
                                                                                                                                                                          tCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCggca                                                                                                                        >  1:244794/1‑169 (MQ=25)
                                                                                                                                                                          tCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCg                                                                                        >  1:126906/1‑203 (MQ=14)
                                                                                                                                                                          cccTGAGCAATCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCgaga                                                                                                                                       <  2:283297/155‑1 (MQ=14)
                                                                                                                                                                          cccTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                                                                                                           <  2:691969/71‑1 (MQ=38)
                                                                                                                                                                          cccTGAGCAACCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAg                                                                                                                                                                                                                              >  2:614429/2‑69 (MQ=16)
                                                                                                                                                                            cTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCa                                                                                                                                                                                                                                          <  2:530912/55‑1 (MQ=35)
                                                                                                                                                                            cTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCa                                                                                                                                                                                                                                          >  1:530915/1‑55 (MQ=38)
                                                                                                                                                                            cTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGcgg                                                                                                                                                                                                                           >  2:411341/1‑68 (MQ=14)
                                                                                                                                                                            cTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATc                                                                                                                                           >  1:62746/1‑150 (MQ=17)
                                                                                                                                                                               acagaTCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCa                                                                                                                                                                                               <  2:545893/91‑1 (MQ=255)
                                                                                                                                                                               aGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGACCATACCATCACCGTTGAAGCCGGCAAAACCCAGCGCAGCGTCGATTTCCAGAcccc                                                                                                                                                                                         >  1:233090/1‑101 (MQ=11)
                                                                                                                                                                                gCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACAGCCAAATCATCACCGTTGCAg                                                                                                                                                                                                                              >  1:750836/1‑63 (MQ=11)
                                                                                                                                                                                  aaTCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGCAccc                                                                                               >  1:184668/1‑188 (MQ=37)
                                                                                                                                                                                    tCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGa                                                           <  1:448860/222‑1 (MQ=21)
                                                                                                                                                                                    tCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCGTTACCGTTGAAGc                                                                                                                                                                                                                             >  2:839487/1‑60 (MQ=255)
                                                                                                                                                                                    tCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                                                                                                           <  1:517791/62‑1 (MQ=40)
                                                                                                                                                                                      cTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGCTCGACTGTCAGCGTCACCATCGAGAGCGCCACAGGCGGCAACTTCGAACAACTGACTCCGAATCCGACCCCg                                                                                        >  1:582478/1‑191 (MQ=11)
                                                                                                                                                                                       tGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGcc                                                                                                                                                                                                                            >  1:473523/1‑58 (MQ=1)
                                                                                                                                                                                            aGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCg                                                                                                                                                                                                                           >  1:568121/1‑54 (MQ=11)
                                                                                                                                                                                            aGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                                                                                                           >  1:767974/1‑54 (MQ=255)
                                                                                                                                                                                            aGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                                                                                                           <  2:767971/54‑1 (MQ=255)
                                                                                                                                                                                            aGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCGCCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCt                                                                                     >  2:537411/1‑188 (MQ=255)
                                                                                                                                                                                               cGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCacta                                                   <  2:629415/219‑1 (MQ=255)
                                                                                                                                                                                                 ccGGTAACTGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                                                                                                           >  2:145515/1‑49 (MQ=255)
                                                                                                                                                                                                 ccGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                                                                                                           <  2:135724/49‑1 (MQ=255)
                                                                                                                                                                                                 ccGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                                                                                                           >  1:135725/1‑49 (MQ=255)
                                                                                                                                                                                                 ccGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTg                                                                                                          >  1:742139/1‑162 (MQ=35)
                                                                                                                                                                                                 ccGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGCTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGCAATTTCGAACATCTGACTCCGAACCCGACGCCAGCCCCGAcaa                                                                              >  1:369750/1‑188 (MQ=11)
                                                                                                                                                                                                   ggTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAg                                                                                                                                                                                                                      <  2:557284/52‑1 (MQ=255)
                                                                                                                                                                                                      gaCCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCgg                                                                                                                                                                                       <  2:531753/79‑1 (MQ=17)
                                                                                                                                                                                                                    ccAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAg               >  2:592309/1‑234 (MQ=255)
                                                                                                                                                                                                                      aaCGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGaa                                                                                                  >  1:588229/1‑149 (MQ=32)
                                                                                                                                                                                                                      aaCGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGCTCGACTGTCAGCGTCACCATCTAGAACGCCACAGGCGGt                                                                                                                         >  2:375/1‑126 (MQ=17)
                                                                                                                                                                                                                               aCCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGa                                       <  1:27660/199‑1 (MQ=255)
                                                                                                                                                                                                                               aCCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCAc  <  1:592312/236‑1 (MQ=255)
                                                                                                                                                                                                                                ccATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGaa                                                                                                  <  1:398471/139‑1 (MQ=32)
                                                                                                                                                                                                                                ccATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGaa                                                                                                  >  2:398468/1‑139 (MQ=33)
                                                                                                                                                                                                                                ccATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGccccga                                                                                 >  1:126665/1‑151 (MQ=25)
                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                         
TTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCAC  >  AE015451/197326‑197784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑