| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 199,360 | T→C | 8.2% | A1622A (GCT→GCC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 199,360 | 0 | T | C | 8.2% | 240.5 / 5.3 | 81 | A1622A (GCT→GCC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base T (28/46); new base C (4/3); total (32/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAACAGCACCACCGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATT > AE015451/199144‑199590 | cAACAGCACCACCGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGCAGcc > 1:302873/1‑235 (MQ=25) cAGCACCACCGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCt < 1:398823/214‑1 (MQ=34) caccGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTc < 1:938599/235‑1 (MQ=35) caccGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTc < 1:326712/235‑1 (MQ=37) caccGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTc < 1:642684/235‑1 (MQ=35) cTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCAcc < 1:803775/236‑1 (MQ=39) tGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCa < 2:513742/233‑1 (MQ=39) tAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCt < 2:554130/228‑1 (MQ=40) gACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGACGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGcc > 1:938009/1‑212 (MQ=38) cTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTg < 2:481587/235‑1 (MQ=255) tATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACtt > 1:517834/1‑181 (MQ=39) tATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTc < 2:875583/198‑1 (MQ=37) tATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGc < 1:672276/236‑1 (MQ=255) tATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACGCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTc > 1:875586/1‑198 (MQ=18) tATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAACCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACtt < 2:517831/181‑1 (MQ=255) tGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCg < 2:91411/181‑1 (MQ=37) tGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGcc > 1:604120/1‑235 (MQ=255) tCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGCCCCa > 1:413296/1‑235 (MQ=255) ggCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGa < 1:257408/236‑1 (MQ=17) gCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCAc < 1:545413/233‑1 (MQ=255) accaaccaGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCaa > 2:876609/1‑215 (MQ=40) accaaccaGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAg < 1:640254/219‑1 (MQ=255) accaaccaGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACGCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAg > 2:640251/1‑219 (MQ=255) caacgcgCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCtt > 2:257405/7‑198 (MQ=18) caaccaGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGTACGCCAGGCAACGAAGGCGATATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTgg > 1:600212/1‑236 (MQ=255) aGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTg < 1:821679/236‑1 (MQ=40) aGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAg < 2:759999/212‑1 (MQ=11) aGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAg > 1:760002/1‑212 (MQ=21) tCAACGCCCTCGACGCGGTCAGCGGCGCAGATGCCTGGAAGTTCGAAAACCTGACCCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTg < 2:119159/191‑1 (MQ=255) cAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACGCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCaa > 1:907981/1‑198 (MQ=39) aaTGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGcaa > 1:650785/1‑236 (MQ=39) ccATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACGCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCaa > 1:350653/1‑193 (MQ=21) cATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCaacaac < 1:911439/235‑1 (MQ=37) aCGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCAc > 1:675214/1‑203 (MQ=17) aCGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCa > 2:488304/1‑235 (MQ=21) gTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCAc < 2:907978/198‑1 (MQ=40) gCAAGGAATTTCTAAAACCTGAACCAGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCAAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGc < 2:684668/131‑1 (MQ=14) aaGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTgg < 1:835785/227‑1 (MQ=40) aaGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACGCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTgg > 2:835782/1‑227 (MQ=255) ggtCGAAAACCTGAACCCGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCa < 2:845368/231‑1 (MQ=255) tCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAgcgcg < 1:450822/236‑1 (MQ=255) aaaCCTGACCCTGGCCAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAgcgcg < 2:868626/232‑1 (MQ=255) ccctgaacctgGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCa < 2:848410/209‑1 (MQ=40) aaGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACgcgc > 1:286916/1‑235 (MQ=255) aGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCa < 2:868691/195‑1 (MQ=255) aGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCa > 1:868694/1‑195 (MQ=40) ccccGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTgg < 2:938006/156‑1 (MQ=35) cccGGTCAGCACCGAGGTCACCGACGAGCCAGGTACGCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCAc < 1:418201/148‑1 (MQ=255) cccGGTCAGCACCGAGGTCACCGACGAGCCAGGTACGCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCAc > 2:418198/1‑148 (MQ=255) cccGGTCAGCACCGAGGTCACCGACGAGCCAGGTACGCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCCCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAg > 1:909932/1‑193 (MQ=255) cccGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCa < 1:688960/180‑1 (MQ=40) ccGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGc < 1:551087/234‑1 (MQ=255) cGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGAt < 1:487107/236‑1 (MQ=255) cGGTAAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCAAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACg < 2:388792/174‑1 (MQ=255) gTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGt < 1:81165/119‑1 (MQ=39) tCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTgg > 1:892796/1‑150 (MQ=38) tCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTgg < 2:892793/150‑1 (MQ=38) tCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGTg > 2:708392/1‑195 (MQ=17) cAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCaacaac < 1:857512/169‑1 (MQ=37) cACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAg < 2:149273/172‑1 (MQ=39) aCCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAgc > 2:274713/1‑198 (MQ=255) aCCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAgc < 1:274716/198‑1 (MQ=255) gTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTCAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCt > 1:38108/1‑229 (MQ=255) gTCACCGACGAGCCAGGCACGCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCt < 2:100494/63‑1 (MQ=14) tCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCt > 1:934398/1‑179 (MQ=14) cgaagaGCCAGGCACCCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCa < 1:225633/185‑1 (MQ=255) gacgaGCCAGGTACGCCAGGCAACGAAGGCGATATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCa > 1:461540/1‑113 (MQ=255) gacgaGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCa > 2:412615/1‑104 (MQ=38) gacgaGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCa < 1:412618/104‑1 (MQ=38) gacgaGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAgg < 2:626229/212‑1 (MQ=255) cTCCAGGCAACGAAGGAGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTg < 2:860832/125‑1 (MQ=38) cAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCa < 2:350650/223‑1 (MQ=255) ggCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGc < 1:876612/215‑1 (MQ=255) aGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGa < 2:461537/113‑1 (MQ=40) ggCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGc < 2:537417/95‑1 (MQ=39) ggCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGc > 1:537420/1‑95 (MQ=39) ccGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTcggcg < 1:99858/229‑1 (MQ=255) cATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCACCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGtgct < 1:570466/235‑1 (MQ=255) aTCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGtgct > 2:317111/1‑234 (MQ=255) tCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTcg > 2:593573/1‑212 (MQ=255) cACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCt > 1:605365/1‑124 (MQ=255) cACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCt < 2:605362/124‑1 (MQ=255) ccGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCAtt < 1:315220/235‑1 (MQ=255) | CAACAGCACCACCGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATT > AE015451/199144‑199590 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |