| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 199,550 | T→C | 12.3% | L1686L (TTG→CTG) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 199,550 | 0 | T | C | 12.3% | 152.6 / 7.4 | 56 | L1686L (TTG→CTG) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base T (19/30); new base C (3/4); total (22/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
TCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAAC‑CAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTG > AE015451/199338‑199771 | ccGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAAC‑CAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTcggcg < 1:99858/229‑1 (MQ=255) cATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAAC‑CACCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGtgct < 1:570466/235‑1 (MQ=255) aTCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAAC‑CAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGtgct > 2:317111/1‑234 (MQ=255) tCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAAC‑CAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTcg > 2:593573/1‑212 (MQ=255) ccGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAAC‑CAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCAtt < 1:315220/235‑1 (MQ=255) aCCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAAC‑CAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCgaga < 2:650782/234‑1 (MQ=255) gagaACGTCAAGCCGACCTGCACCGTGCACGTCAAC‑CAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGc < 2:684677/197‑1 (MQ=255) aaGCCGACCTTCACCGTGCACGTCAAC‑CAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCgg < 1:317114/235‑1 (MQ=255) ccGACCTTCACTGTGCACATCAACACCGCC‑CTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGtgct < 2:462271/195‑1 (MQ=11) tCAAC‑CAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCtt < 2:413293/191‑1 (MQ=255) aaC‑CAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCCGTCGACGCCACTgg > 1:500061/1‑170 (MQ=255) aaccccccGCTGGCACACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAAc < 2:909929/180‑1 (MQ=255) aGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGtgct < 2:371438/200‑1 (MQ=255) aCCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCgg > 2:544787/1‑181 (MQ=255) gCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCt > 2:173805/1‑157 (MQ=255) gCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCt < 1:173807/157‑1 (MQ=255) caacaaCGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGttccg > 2:910590/1‑163 (MQ=255) aacaacGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGa > 2:459301/1‑152 (MQ=255) aacaacGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCg < 1:785429/235‑1 (MQ=255) aacaacGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGa > 1:756220/1‑236 (MQ=255) ccAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTg < 1:428674/236‑1 (MQ=255) aGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGGGGACGCCACTGGtg > 2:251537/1‑123 (MQ=25) aGGTCACCATCAAGGCGGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGt < 2:604117/233‑1 (MQ=255) aTCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTcggcg < 2:588201/99‑1 (MQ=37) gaCCAGCGCGACGTACACCCACGACGCGCAAGTCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCAccc < 2:600209/233‑1 (MQ=255) ccAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCGGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACTCCGTCGGTCACCGAAGGCggc < 1:523838/209‑2 (MQ=255) cgcgcCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACcaaca > 1:433032/1‑236 (MQ=18) cgcCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCAc < 1:393068/211‑1 (MQ=255) gcCGTACACCCGCGACGCGCAAGGCGATGCCGGCTATCAGGACGCTGGCCAGACCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGtt < 2:201761/133‑1 (MQ=255) cGTACACTCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGAc < 1:335974/236‑1 (MQ=38) tACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGACGTGGTGGCCAAGCTGACt > 2:746965/1‑171 (MQ=255) acacCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTGCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTg < 1:626976/198‑1 (MQ=14) cacCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCaa < 2:521012/162‑1 (MQ=255) ctcACGACGCGCAGGGCGATGACGTCTATCAGGATGCGGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTCGAGCTGGGCGGTGCTGCGTCGGTGCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCAccccct > 2:824735/3‑175 (MQ=11) ccACGACGCGCAAGGCGATGACGTCTAGCAGGACGCTGGCCAGATCAGCTTGGGCATCAACGCGGCGGTGGACGCCACTGGTGCTGCAGTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCAc < 2:286913/201‑1 (MQ=255) cACGACGCGCAAGGCGATGACGTCTATCAGGACGCGGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTcg < 2:117033/142‑1 (MQ=255) cgacgCGCAGGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCAcc < 1:242344/211‑1 (MQ=255) cgacgCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCAcc > 1:450651/1‑211 (MQ=255) cgacgCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCAcc < 2:450648/211‑1 (MQ=40) gacgCGCAGGGCGATGACGTCTATCAGGATGCGGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCg < 2:601858/181‑1 (MQ=255) cgcgCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCg > 2:929227/1‑185 (MQ=255) cgcAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCaac > 1:494471/1‑235 (MQ=39) cgcAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGGGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTga > 1:198679/1‑187 (MQ=255) cAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCaa < 2:494468/233‑1 (MQ=17) aaGGCGATGACGTCTATCAGGACGCTGGCCAGATCGGCTTGGGCCTCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAg > 2:548538/1‑92 (MQ=255) ggCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGCAGGCGGTGAGATCAcc > 1:893897/1‑189 (MQ=255) cGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAAc > 2:366146/1‑232 (MQ=255) acatCTATCAGGATGCGGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTga < 1:563441/172‑1 (MQ=255) cGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACGCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCGTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCAc < 1:16446/229‑1 (MQ=255) tCTATCAGGATGCGGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTCGAGTTGGGCGGTGCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCGTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTg < 2:758939/212‑1 (MQ=255) tCAGGACGCTGGCCAGATCAGCTTGGGCATCAGCTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTaca < 1:288665/179‑1 (MQ=255) cTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACcaacaa > 2:48136/1‑190 (MQ=18) tGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCCGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTg < 1:854312/235‑1 (MQ=255) gCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCaa < 1:411567/205‑1 (MQ=255) gCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCaa > 2:411564/1‑205 (MQ=255) gCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTc > 2:399552/1‑227 (MQ=255) ttGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCAt < 1:290730/40‑1 (MQ=37) | TCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAAC‑CAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTG > AE015451/199338‑199771 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |