| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 201,373 | T→C | 12.0% | T2293T (ACT→ACC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 201,373 | 0 | T | C | 12.0% | 142.8 / 2.8 | 48 | T2293T (ACT→ACC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base T (20/22); new base C (3/3); total (23/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
TGCAACCGCACCAGACAACGTCTATGTCGGCACTAATGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTGCAA > AE015451/201139‑201574 | tGCAACCGCACCAGACAACGTCTATGTCGGCACTAATGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACt < 1:518866/235‑1 (MQ=255) gTCGGCACTAATGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTgg < 2:590055/232‑1 (MQ=255) tCGGCACTAATGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCt > 1:350332/1‑229 (MQ=255) ggCACTAATGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCACCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGa < 1:505125/236‑1 (MQ=255) gtggtCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCt < 1:724443/236‑1 (MQ=255) gtCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAg > 1:162311/1‑236 (MQ=255) cATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGTCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGc > 2:864854/1‑229 (MQ=255) tCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCAcc < 1:108861/236‑1 (MQ=40) ggcgCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCa < 1:927962/235‑1 (MQ=255) gcAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGc < 2:672429/185‑1 (MQ=255) gcAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGc > 1:672432/1‑185 (MQ=40) aGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACTATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACCGTACACATCaac > 2:644661/1‑171 (MQ=255) aTGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGcc > 2:554779/1‑175 (MQ=39) gTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAgc < 1:600816/235‑1 (MQ=255) tgaacctgGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGc < 1:554782/160‑1 (MQ=255) caggACAACACCCCGGTCAGCCCCGACGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACAccc < 1:225198/230‑1 (MQ=255) aaGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACAATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAgcg > 1:580641/1‑214 (MQ=255) tCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGcaac > 2:672740/1‑167 (MQ=255) tCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGAc > 1:43524/1‑236 (MQ=255) ccGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCaacaac < 2:820517/161‑1 (MQ=255) tgaccgACGAGCCAGGCACACCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGa < 2:657798/133‑1 (MQ=255) tgaccaaCGAGCCAGGCACACCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAgcgcg < 1:689496/188‑1 (MQ=255) ggCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCa > 1:747249/1‑235 (MQ=255) aCTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCaacaac > 2:361041/1‑141 (MQ=255) aCTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCaacaac < 1:361044/141‑1 (MQ=255) gCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGccc > 2:2657/1‑136 (MQ=255) aaCGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAgcg < 2:762187/166‑1 (MQ=255) ggCGACTTGGTCAAGGTCACCATCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCt > 1:207694/1‑207 (MQ=11) ggCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGCCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGGACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCt > 1:271859/1‑207 (MQ=255) aTCGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGAt < 2:609572/183‑1 (MQ=25) gTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGc < 1:663013/236‑1 (MQ=255) tCACCATCACGGCCGACCAGGCTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGt < 1:897167/236‑1 (MQ=255) aCCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGAcgc < 2:142532/161‑1 (MQ=255) ccATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGcc > 2:59917/1‑70 (MQ=255) ccATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGcc < 1:59917/70‑1 (MQ=255) tCACGGCCGAACAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCaacaac < 2:629457/104‑1 (MQ=255) ccGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAg > 2:736900/1‑173 (MQ=255) cGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCg > 2:688749/1‑158 (MQ=40) aCCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACg > 2:34369/1‑174 (MQ=255) cAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGAt > 2:936502/1‑156 (MQ=255) cAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGAt < 1:936505/156‑1 (MQ=255) cTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTGCaa < 1:742789/235‑1 (MQ=255) aaCGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGc < 2:913842/155‑1 (MQ=255) aaCGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCCTCAACTCGGCGGTGGACGCCACTGGTGCTGCGTTCga > 1:267139/1‑212 (MQ=255) aaCGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCt > 1:639093/1‑218 (MQ=255) aaCGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGc > 1:913845/1‑155 (MQ=255) aCGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCGtt < 1:719281/208‑1 (MQ=25) tCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGGGCAACACCGCTCAGGTCACCATCAAGGCCggc > 2:806791/1‑82 (MQ=255) tCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCGtt < 1:919013/193‑1 (MQ=255) | TGCAACCGCACCAGACAACGTCTATGTCGGCACTAATGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTGCAA > AE015451/201139‑201574 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |