Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AE015451 201,385 T→C 11.1% N2297N (AAT→AAC)  PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451201,3850TC11.1% 161.2 / 5.0 52N2297N (AAT→AAC) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base T (23/23);  new base C (2/4);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCGGCACTAATGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGA  >  AE015451/201164‑201618
                                                                                                                                                                                                                             |                                                                                                                                                                                                                                         
gTCGGCACTAATGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTgg                                                                                                                                                                                                                                 <  2:590055/232‑1 (MQ=255)
 tCGGCACTAATGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCt                                                                                                                                                                                                                                   >  1:350332/1‑229 (MQ=255)
   ggCACTAATGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCACCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGa                                                                                                                                                                                                                          <  1:505125/236‑1 (MQ=255)
                  gtggtCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCt                                                                                                                                                                                                           <  1:724443/236‑1 (MQ=255)
                     gtCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAg                                                                                                                                                                                                        >  1:162311/1‑236 (MQ=255)
                             cATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGTCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGc                                                                                                                                                                                                       >  2:864854/1‑229 (MQ=255)
                                        tCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCAcc                                                                                                                                                                                     <  1:108861/236‑1 (MQ=40)
                                             ggcgCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCa                                                                                                                                                                                 <  1:927962/235‑1 (MQ=255)
                                                gcAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGc                                                                                                                                                                                                                                >  1:672432/1‑185 (MQ=40)
                                                gcAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGc                                                                                                                                                                                                                                <  2:672429/185‑1 (MQ=255)
                                                    aTGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGcc                                                                                                                                                                                                                                      >  2:554779/1‑175 (MQ=39)
                                                              gTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAgc                                                                                                                                                                <  1:600816/235‑1 (MQ=255)
                                                                         tgaacctgGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGc                                                                                                                                                                                                                                <  1:554782/160‑1 (MQ=255)
                                                                              caggACAACACCCCGGTCAGCCCCGACGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACAccc                                                                                                                                                   <  1:225198/230‑1 (MQ=255)
                                                                                    aaGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACAATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAgcg                                                                                                                                                               >  1:580641/1‑214 (MQ=255)
                                                                                              tCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGcaac                                                                                                                                                                                                    >  2:672740/1‑167 (MQ=255)
                                                                                              tCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGAc                                                                                                                               >  1:43524/1‑236 (MQ=255)
                                                                                                       ccGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCaacaac                                                                                                                                                                                                 <  2:820517/161‑1 (MQ=255)
                                                                                                          tgaccgACGAGCCAGGCACACCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGa                                                                                                                                                                                                                     <  2:657798/133‑1 (MQ=255)
                                                                                                          tgaccaaCGAGCCAGGCACACCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAgcgcg                                                                                                                                                             <  1:689496/188‑1 (MQ=255)
                                                                                                                        ggCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCa                                                                                                      >  1:747249/1‑235 (MQ=255)
                                                                                                                           aCTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCaacaac                                                                                                                                                                                                 <  1:361044/141‑1 (MQ=255)
                                                                                                                           aCTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCaacaac                                                                                                                                                                                                 >  2:361041/1‑141 (MQ=255)
                                                                                                                                  gCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGccc                                                                                                                                                                                             >  2:2657/1‑136 (MQ=255)
                                                                                                                                    aaCGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAgcg                                                                                                                                                               <  2:762187/166‑1 (MQ=255)
                                                                                                                                          ggCGACTTGGTCAAGGTCACCATCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCt                                                                                                                >  1:207694/1‑207 (MQ=11)
                                                                                                                                          ggCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGCCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGGACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCt                                                                                                                >  1:271859/1‑207 (MQ=255)
                                                                                                                                                aTCGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGAt                                                                                                                                  <  2:609572/183‑1 (MQ=25)
                                                                                                                                                   gTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGc                                                                          <  1:663013/236‑1 (MQ=255)
                                                                                                                                                          tCACCATCACGGCCGACCAGGCTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGt                                                                   <  1:897167/236‑1 (MQ=255)
                                                                                                                                                            aCCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGAcgc                                                                                                                                            <  2:142532/161‑1 (MQ=255)
                                                                                                                                                             ccATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGcc                                                                                                                                                                                                                                      >  2:59917/1‑70 (MQ=255)
                                                                                                                                                             ccATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGcc                                                                                                                                                                                                                                      <  1:59917/70‑1 (MQ=255)
                                                                                                                                                                tCACGGCCGAACAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCaacaac                                                                                                                                                                                                 <  2:629457/104‑1 (MQ=255)
                                                                                                                                                                      ccGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAg                                                                                                                      >  2:736900/1‑173 (MQ=255)
                                                                                                                                                                       cGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCg                                                                                                                                    >  2:688749/1‑158 (MQ=40)
                                                                                                                                                                         aCCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACg                                                                                                                  >  2:34369/1‑174 (MQ=255)
                                                                                                                                                                           cAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGAt                                                                                                                                  >  2:936502/1‑156 (MQ=255)
                                                                                                                                                                           cAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGAt                                                                                                                                  <  1:936505/156‑1 (MQ=255)
                                                                                                                                                                                cTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTGCaa                                              <  1:742789/235‑1 (MQ=255)
                                                                                                                                                                                             aaCGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGc                                                                                                                 <  2:913842/155‑1 (MQ=255)
                                                                                                                                                                                             aaCGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCCTCAACTCGGCGGTGGACGCCACTGGTGCTGCGTTCga                                                        >  1:267139/1‑212 (MQ=255)
                                                                                                                                                                                             aaCGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCt                                                  >  1:639093/1‑218 (MQ=255)
                                                                                                                                                                                             aaCGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGc                                                                                                                 >  1:913845/1‑155 (MQ=255)
                                                                                                                                                                                              aCGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCGtt                                                           <  1:719281/208‑1 (MQ=25)
                                                                                                                                                                                                             tCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGGGCAACACCGCTCAGGTCACCATCAAGGCCggc                                                                                                                                                                         >  2:806791/1‑82 (MQ=255)
                                                                                                                                                                                                             tCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCGtt                                                           <  1:919013/193‑1 (MQ=255)
                                                                                                                                                                                                                   cgCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTGCAACTGGGCGGCAACGCt                               <  2:916446/214‑1 (MQ=255)
                                                                                                                                                                                                                           aaTCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCa                                                                                                                                                                  >  2:230631/1‑76 (MQ=255)
                                                                                                                                                                                                                           aaTCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCa                                                                                                                                                                  <  1:230633/76‑1 (MQ=255)
                                                                                                                                                                                                                           aaTCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTcgacga  >  1:681601/1‑236 (MQ=255)
                                                                                                                                                                                                                           aaTCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTcgac    >  1:604113/1‑234 (MQ=255)
                                                                                                                                                                                                                             |                                                                                                                                                                                                                                         
GTCGGCACTAATGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAATCAGCCGCTGGCCCACGACCTGATCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGA  >  AE015451/201164‑201618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑