| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 202,581 | A→C | 5.2% | E2696A (GAG→GCG) ‡ | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 202,581 | 0 | A | C | 5.2% | 253.8 / 3.5 | 73 | E2696A (GAG→GCG) ‡ | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base A (24/45); new base C (2/2); total (26/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.56e-01 | |||||||||||
CCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTA > AE015451/202347‑202812 | ccAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGTACCAACTCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAg > 1:911018/1‑236 (MQ=25) caacaaCCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCa > 2:478450/1‑233 (MQ=40) acaacCACTCGGAGCTGTACTTCAAGCTGACCGATGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAGCCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTcc < 1:667413/235‑1 (MQ=255) acaacCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTcc < 1:698330/235‑1 (MQ=40) gAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGc > 1:838612/1‑228 (MQ=255) aCTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGc < 1:905435/230‑1 (MQ=255) tCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCATGGAAGTTCGCAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACTAAGGCGATATCg < 2:522095/234‑1 (MQ=255) gACCGGCGGCACCCCCGTCGTCGTGGCAGCCAACCGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCaaa < 1:2507/232‑1 (MQ=255) ccGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCa > 2:702468/1‑197 (MQ=40) tgtcgtcgtGGCAGCCAACAGCACCACCGGTTCGGCCACTGTAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCaaa < 1:847905/216‑1 (MQ=21) gCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGt < 2:805254/233‑1 (MQ=255) gCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGc > 1:842769/1‑236 (MQ=255) cAGCACCCCCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGc < 1:245218/164‑1 (MQ=255) ggTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAAcc < 1:536630/236‑1 (MQ=255) ggCCACTGCCACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAACGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCGGGCACCCCAGGCAACGAAGGc < 1:211694/172‑1 (MQ=255) gCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGaacg > 2:509164/1‑168 (MQ=255) cACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCCTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGActt < 2:581753/233‑3 (MQ=255) aCTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAAcc < 2:699143/227‑1 (MQ=255) cTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAAcc < 2:782721/226‑1 (MQ=255) cAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCAcc < 2:411483/233‑1 (MQ=255) aaCCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCg < 2:143343/170‑1 (MQ=255) ccgccgCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCAcc < 1:439787/230‑1 (MQ=255) gCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTTGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCt < 1:57972/223‑1 (MQ=255) gACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACGGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACAGCTGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCg > 1:746394/1‑223 (MQ=255) gACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTa > 2:83054/1‑225 (MQ=255) cAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCg > 1:427888/1‑236 (MQ=255) cAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCg < 1:881144/236‑1 (MQ=255) tCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAgg < 2:326770/127‑1 (MQ=255) cTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGGGGCCGAGAACGTCaa > 2:519896/1‑201 (MQ=255) gagggcGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCg < 2:62285/146‑1 (MQ=255) cGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGGATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACGCCAGGCAACGAAGGCGa > 2:922888/1‑141 (MQ=255) ggCACCAACGCGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGc < 2:427885/230‑1 (MQ=255) ccccAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTActgct < 2:711374/161‑1 (MQ=255) cccAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGa < 1:102006/232‑1 (MQ=255) aCCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCGGGCACCCCAGGCAACGAAGGCGacttggtc < 2:672504/144‑8 (MQ=255) aCCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCGGGCACCCCAGGCAACGAAGGCGacttggtc > 1:672507/1‑137 (MQ=255) aCCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGa > 2:100017/1‑131 (MQ=255) aCCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCt > 1:620447/1‑236 (MQ=255) cAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACat > 2:756303/1‑208 (MQ=255) aCGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCCGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGCGGCCGAGAACGCCAAACCGACCTTCACCGTACATATCAACACCGCCtt > 2:270075/1‑220 (MQ=255) aCGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGt < 2:268044/172‑1 (MQ=255) gcgcCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACGGAGGTCACTGACGAGCCAGGCACTCCa > 2:109558/1‑117 (MQ=255) gtCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGAc > 1:689400/1‑155 (MQ=1) gtCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGAc < 2:689397/155‑1 (MQ=11) cATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACg > 1:653015/1‑236 (MQ=255) tCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCt < 1:705669/174‑1 (MQ=255) gACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCg < 2:719476/193‑1 (MQ=255) cGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAgg > 1:608282/1‑236 (MQ=255) tCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTg < 2:488057/209‑1 (MQ=255) cAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGa < 2:818860/161‑1 (MQ=255) aTGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGaa > 2:267771/1‑86 (MQ=255) aTGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGaa < 1:267774/86‑1 (MQ=255) tGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCaacaac < 2:484564/211‑1 (MQ=255) gTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCg < 2:752846/170‑1 (MQ=255) aaGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAGGTCACCATTACTGCTGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGtt > 2:380987/1‑152 (MQ=11) aaaaCCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGcc > 2:737108/1‑218 (MQ=255) aaaCCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTg < 2:510800/163‑1 (MQ=255) gAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACAccc < 1:209360/236‑1 (MQ=255) gAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACAccc < 1:756306/236‑1 (MQ=255) tGGACAAGACCGCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAgcgcg < 2:846812/221‑1 (MQ=255) tGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGcc < 2:838609/206‑1 (MQ=255) ggACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCGCCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTg < 1:651975/236‑1 (MQ=255) gACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACa < 2:16023/134‑1 (MQ=255) cAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTgcg < 1:702471/235‑1 (MQ=255) cccGGTCAGCACTGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTGCTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCt < 2:143239/116‑1 (MQ=255) cccGGTCAGCACCGAGGTCACTGACGAGCCAGTCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCGGACCATACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACg > 1:709787/1‑224 (MQ=255) cccGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCt < 2:162098/116‑1 (MQ=255) cccGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGt < 1:598414/235‑1 (MQ=255) ccGGTCAGCACCGAGGTCGCTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACg < 2:192014/175‑1 (MQ=255) ggTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTa > 2:626881/1‑213 (MQ=255) aCCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTa > 1:755456/1‑236 (MQ=255) ccGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCt < 1:571000/215‑1 (MQ=255) gAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCATCACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTa < 2:869605/233‑1 (MQ=255) | CCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTA > AE015451/202347‑202812 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |