Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	205,465	G→A	7.3%	P3657P (CCG→CCA)	PP_0168 →	putative surface adhesion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	205,465	0	G	A	7.3%	179.6 / 4.9	59	P3657P (CCG→CCA)	PP_0168	putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base G (22/32);  new base A (2/2);  total (25/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01

GGTCAGCACTACCGTCACCGACGAGCCTGGTACCCCAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACA  >  AE015451/205240‑205694                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                      ggTCAGCACTACCGTCACCGACGAGCCTGGTACCCCAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCCTACACCCa                                                                                                                                                                                                                               >  2:496165/1‑234 (MQ=18)     aGCACTACCGTCACCGACGAGCCTGGTACCCCAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTg                                                                                                                                                                                                                          >  1:831700/1‑235 (MQ=34)          tACCGTCACCGACGAGCCTGGTACCCCAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTgcg                                                                                                                                                                                                                        >  1:292142/1‑232 (MQ=34)               tCACCGACGAGCCTGGTACCCCAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGc                                                                                                                                                                                                                  <  2:831697/233‑1 (MQ=38)                              gTACCCCAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACg                                                                                                                                                                                                  <  2:498536/234‑1 (MQ=39)                               tACCCCAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACg                                                                                                                                                                                                  <  2:323646/233‑1 (MQ=39)                                      ggCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCa                                                                                                                                                                                        <  1:811767/236‑1 (MQ=255)                                       gCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTgc                                                                                                                                                                                                                         >  2:396123/1‑202 (MQ=35)                                          aCCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGACCAGATc                                                                                                                                                                                    <  1:679037/236‑1 (MQ=255)                                            ccGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGc                                                                                                                                                                                                                  <  2:936678/204‑1 (MQ=18)                                                gcggcAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCa                                                                                                                                                                                   <  2:365619/231‑1 (MQ=38)                                                            aaGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCacgtc                                                                                                                                                                   <  1:757764/235‑5 (MQ=9)                                                             aGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGcc                                                                                                                                                                                         <  2:518359/212‑1 (MQ=35)                                                                         cAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGAt                                                                                                                                                                                                               <  2:803405/178‑1 (MQ=12)                                                                         cAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGAt                                                                                                                                                                                                               >  1:803408/1‑178 (MQ=37)                                                                                aCCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACg                                                                                                                                                                                                  <  1:171273/184‑1 (MQ=37)                                                                                     cACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGAt                                                                                                                                                                                                               >  2:757418/1‑166 (MQ=16)                                                                                      aCCGCCGCCCAGACTCCGGTAGCCGAGAACGTCAAACCGCCCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCt                                                                                                                                                                                             <  2:101366/183‑1 (MQ=255)                                                                                      aCCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCTGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCtt                                                                                                                                        <  1:511548/236‑1 (MQ=38)                                                                                       ccgccgACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACt                                                                                                                                                                    <  2:928162/207‑1 (MQ=35)                                                                                        ggccgACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAg                                                                                                                                                                                       <  2:478872/186‑1 (MQ=21)                                                                                           cgACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTa                                                                                                                                                                                                       >  1:900691/1‑168 (MQ=37)                                                                                           cgACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTa                                                                                                                                                                                                       <  2:900688/168‑1 (MQ=37)                                                                                             aCCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCAGGCTgc                                                                                                                                                                                                                         >  1:161927/1‑148 (MQ=17)                                                                                               cAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGcctg                                                                                                                                                                                                               >  1:202566/1‑153 (MQ=14)                                                                                               cAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACg                                                                                                                                                                                                           >  2:235227/1‑160 (MQ=35)                                                                                                           gCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGggcgg                                                                                                                   <  1:71364/236‑1 (MQ=38)                                                                                                                       aaaCCGACCTTACCTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATg                                                                                                                                                        <  2:635073/187‑1 (MQ=37)                                                                                                                        aaCCGCCCTTCCCTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCGGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCa                                                                                                                                                                                   <  2:98320/159‑1 (MQ=255)                                                                                                                              ccTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGt                                                                                                 <  1:481093/235‑1 (MQ=255)                                                                                                                                ttCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGAc                                                                                              >  1:786711/1‑236 (MQ=40)                                                                                                                                      gTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCCCCGTACACCCACGCTGCGCAGGGc                                                                                                                                                                                                                  <  1:517440/114‑1 (MQ=17)                                                                                                                                               aaCCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGAc                                                                                                                                           >  2:532886/1‑176 (MQ=33)                                                                                                                                                    gCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACcg                                                                                   <  1:312616/227‑1 (MQ=39)                                                                                                                                                             tCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAg                                                                             >  2:437469/1‑224 (MQ=40)                                                                                                                                                              cACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTc                                                                                                     >  2:485973/1‑199 (MQ=40)                                                                                                                                                                         cGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCCACGCTTCGGTTCaa                                                                                                   >  1:217426/1‑190 (MQ=255)                                                                                                                                                                           tGACCCTGAGCAACACCGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACcg                                                                                   <  1:634815/204‑1 (MQ=255)                                                                                                                                                                             aCCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGt                                                  <  1:583978/235‑1 (MQ=255)                                                                                                                                                                                cTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTACAACTGGGTGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCg                                                >  1:523686/1‑234 (MQ=255)                                                                                                                                                                                     caacaaTGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTc                                                                                                          >  2:440974/1‑171 (MQ=255)                                                                                                                                                                                     caacaaCGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGGTCAAGTGACCGACACCACCGACGAAGGGGGGGc                                                                      >  1:165177/1‑207 (MQ=255)                                                                                                                                                                                          gcGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTTCAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGGCGGTCACCGAAgg                                     <  1:575854/234‑1 (MQ=255)                                                                                                                                                                                           cGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGaa                                       >  1:411536/1‑232 (MQ=40)                                                                                                                                                                                              ccAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCTCAGGGTGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCACCTCGGCTGTGGATGCCACTGGTGCCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAggcggc                                 <  1:420315/235‑1 (MQ=255)                                                                                                                                                                                              ccAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAggcggc                                 <  1:916866/235‑1 (MQ=40)                                                                                                                                                                                                aGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACCCCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAggcggc                                 <  1:255974/233‑1 (MQ=38)                                                                                                                                                                                                   tCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCg                                                                                            >  2:858236/1‑171 (MQ=40)                                                                                                                                                                                                      ccATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCAc                         <  1:532889/235‑1 (MQ=39)                                                                                                                                                                                                          cAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAggcggc                                 <  1:846136/223‑1 (MQ=39)                                                                                                                                                                                                            aGGCCGGTAAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGAcc                                                            <  2:556868/194‑1 (MQ=255)                                                                                                                                                                                                               ccGGTGAAACCAGCGCGCCGTACACCCACGCTGCTCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGGAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCAc                         >  2:478112/1‑226 (MQ=255)                                                                                                                                                                                                               ccGGTGAAACCAGCGCGCCGTACACCCACGCTGCTCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCAc                         <  1:478115/226‑1 (MQ=255)                                                                                                                                                                                                               ccGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGCCGAAg                                                                             >  2:141275/1‑174 (MQ=255)                                                                                                                                                                                                               ccGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCgaga                             >  1:53148/1‑222 (MQ=39)                                                                                                                                                                                                                    gAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACCGGt                                                                                                                                                >  2:465462/1‑102 (MQ=25)                                                                                                                                                                                                                             gcgcCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACcaaca  <  1:418868/235‑1 (MQ=39)                                                                                                                                                                                                                              cgcCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCa                 <  1:494690/219‑1 (MQ=40)                                                                                                                                                                                                                               gcCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTggag                                                                                                                           >  2:747606/1‑109 (MQ=25)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                      GGTCAGCACTACCGTCACCGACGAGCCTGGTACCCCAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACA  >  AE015451/205240‑205694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑