Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	205,504	T→C	7.3%	D3670D (GAT→GAC)	PP_0168 →	putative surface adhesion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	205,504	0	T	C	7.3%	175.1 / 4.3	55	D3670D (GAT→GAC)	PP_0168	putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base T (20/31);  new base C (2/2);  total (22/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-01

GGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCAT  >  AE015451/205277‑205727                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                ggCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCa                                                                                                                                                                                                                         <  1:811767/236‑1 (MQ=255)     aCCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGACCAGATc                                                                                                                                                                                                                     <  1:679037/236‑1 (MQ=255)           gcggcAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCa                                                                                                                                                                                                                    <  2:365619/231‑1 (MQ=38)                       aaGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCacgtc                                                                                                                                                                                                    <  1:757764/235‑5 (MQ=9)                        aGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGcc                                                                                                                                                                                                                          <  2:518359/212‑1 (MQ=35)                                                 aCCGCCGCCCAGACTCCGGTAGCCGAGAACGTCAAACCGCCCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCt                                                                                                                                                                                                                              <  2:101366/183‑1 (MQ=255)                                                 aCCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCTGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCtt                                                                                                                                                                         <  1:511548/236‑1 (MQ=38)                                                  ccgccgACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACt                                                                                                                                                                                                     <  2:928162/207‑1 (MQ=35)                                                   ggccgACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAg                                                                                                                                                                                                                        <  2:478872/186‑1 (MQ=21)                                                                      gCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGggcgg                                                                                                                                                    <  1:71364/236‑1 (MQ=38)                                                                                  aaaCCGACCTTACCTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATg                                                                                                                                                                                         <  2:635073/187‑1 (MQ=37)                                                                                   aaCCGCCCTTCCCTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCGGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCa                                                                                                                                                                                                                    <  2:98320/159‑1 (MQ=255)                                                                                         ccTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGt                                                                                                                                  <  1:481093/235‑1 (MQ=255)                                                                                           ttCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGAc                                                                                                                               >  1:786711/1‑236 (MQ=40)                                                                                                          aaCCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGAc                                                                                                                                                                            >  2:532886/1‑176 (MQ=33)                                                                                                               gCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACcg                                                                                                                    <  1:312616/227‑1 (MQ=39)                                                                                                                        tCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAg                                                                                                              >  2:437469/1‑224 (MQ=40)                                                                                                                         cACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTc                                                                                                                                      >  2:485973/1‑199 (MQ=40)                                                                                                                                    cGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCCACGCTTCGGTTCaa                                                                                                                                    >  1:217426/1‑190 (MQ=255)                                                                                                                                      tGACCCTGAGCAACACCGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACcg                                                                                                                    <  1:634815/204‑1 (MQ=255)                                                                                                                                        aCCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGt                                                                                   <  1:583978/235‑1 (MQ=255)                                                                                                                                           cTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTACAACTGGGTGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCg                                                                                 >  1:523686/1‑234 (MQ=255)                                                                                                                                                caacaaTGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTc                                                                                                                                           >  2:440974/1‑171 (MQ=255)                                                                                                                                                caacaaCGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGGTCAAGTGACCGACACCACCGACGAAGGGGGGGc                                                                                                       >  1:165177/1‑207 (MQ=255)                                                                                                                                                     gcGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTTCAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGGCGGTCACCGAAgg                                                                      <  1:575854/234‑1 (MQ=255)                                                                                                                                                      cGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGaa                                                                        >  1:411536/1‑232 (MQ=40)                                                                                                                                                         ccAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCTCAGGGTGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCACCTCGGCTGTGGATGCCACTGGTGCCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAggcggc                                                                  <  1:420315/235‑1 (MQ=255)                                                                                                                                                         ccAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAggcggc                                                                  <  1:916866/235‑1 (MQ=40)                                                                                                                                                           aGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACCCCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAggcggc                                                                  <  1:255974/233‑1 (MQ=38)                                                                                                                                                              tCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCg                                                                                                                             >  2:858236/1‑171 (MQ=40)                                                                                                                                                                 ccATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCAc                                                          <  1:532889/235‑1 (MQ=39)                                                                                                                                                                     cAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAggcggc                                                                  <  1:846136/223‑1 (MQ=39)                                                                                                                                                                       aGGCCGGTAAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGAcc                                                                                             <  2:556868/194‑1 (MQ=255)                                                                                                                                                                          ccGGTGAAACCAGCGCGCCGTACACCCACGCTGCTCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGGAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCAc                                                          >  2:478112/1‑226 (MQ=255)                                                                                                                                                                          ccGGTGAAACCAGCGCGCCGTACACCCACGCTGCTCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCAc                                                          <  1:478115/226‑1 (MQ=255)                                                                                                                                                                          ccGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGCCGAAg                                                                                                              >  2:141275/1‑174 (MQ=255)                                                                                                                                                                          ccGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCgaga                                                              >  1:53148/1‑222 (MQ=39)                                                                                                                                                                               gAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACCGGt                                                                                                                                                                                 >  2:465462/1‑102 (MQ=25)                                                                                                                                                                                        gcgcCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACcaaca                                   <  1:418868/235‑1 (MQ=39)                                                                                                                                                                                         cgcCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCa                                                  <  1:494690/219‑1 (MQ=40)                                                                                                                                                                                          gcCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTggag                                                                                                                                                            >  2:747606/1‑109 (MQ=25)                                                                                                                                                                                                cccccACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTc                          <  1:194390/234‑1 (MQ=38)                                                                                                                                                                                                        cTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATc                  <  1:598696/236‑1 (MQ=40)                                                                                                                                                                                                         tGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCAccg                                                                                                                                                                                   >  2:247461/1‑72 (MQ=255)                                                                                                                                                                                                           cgcAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCg                     <  2:396749/230‑1 (MQ=40)                                                                                                                                                                                                           cgcAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGGGGTGGCCAAGCTggc                                                                                              >  2:101335/1‑155 (MQ=255)                                                                                                                                                                                                              aGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGa                                                                                                                            <  2:130319/124‑1 (MQ=40)                                                                                                                                                                                                              aGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGa                                                                                                                            >  1:130320/1‑124 (MQ=40)                                                                                                                                                                                                              aGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCaacaac            >  1:307748/1‑236 (MQ=37)                                                                                                                                                                                                                ggtgATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGAccc                                                                                      <  1:340520/157‑1 (MQ=255)                                                                                                                                                                                                                ggCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAAcc           <  1:340029/235‑1 (MQ=255)                                                                                                                                                                                                                       aCGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGc    <  1:356777/235‑1 (MQ=40)                                                                                                                                                                                                                        cGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCAt  >  1:396549/1‑236 (MQ=40)                                                                                                                                                                                                                        cGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCAt  >  1:455636/1‑236 (MQ=255)                                                                                                                                                                                                                        cGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCAAGTCGGCGGTGGATGCAAATGGTGCGACCTTCGAAAACCTGCAACTGggcg                                                                                                                                                     >  2:769685/1‑89 (MQ=11)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                GGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCAT  >  AE015451/205277‑205727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑