| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 207,343 | T→C | 17.0% | A4283A (GCT→GCC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 207,343 | 0 | T | C | 17.0% | 91.0 / 2.2 | 39 | A4283A (GCT→GCC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base T (12/20); new base C (2/5); total (14/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCACGTCGGTCACCGACGAGCCGGGCACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTG > AE015451/207218‑207571 | aaGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCACGTCGGTCACCGACGAGCCGGGCACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAg > 1:588400/1‑236 (MQ=32) aaGACCCCGGTCAGCACGTCGGTCACCGACGAGCCGGGCACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACACCCACGCTgcgc > 1:62050/1‑235 (MQ=33) cccGGTCAGCACGTCGGTCACCGACGAGCCGGGCACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCa < 2:639369/206‑1 (MQ=33) tCAGCACGTCGGTCACCGACGAGCCGGGCACCCCAGGCAAGGAAGGCGACTTGGTCAAGGTCACCATCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAgcgcggc > 1:350619/1‑206 (MQ=17) tCAGCACGTCGGTCACCGACGAGCCGGGCACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGa > 1:164031/1‑235 (MQ=37) cAGCACGTCGGTCACCGACGAGCCGGGCACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAgc > 2:831811/1‑202 (MQ=32) cAGCACGTCGGTCACCGACGAGCCGGGCACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACAGCCGACCAGACATCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCggc > 1:140737/1‑192 (MQ=14) gTCGGTCACCGACGAGCCGGGCACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCACGGCCGGTGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTc > 1:217231/1‑232 (MQ=37) gTCGGTCACCGACGAGCCGGGCACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTg > 1:61938/1‑188 (MQ=32) gacgaGCCGGGCACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATa < 2:394042/194‑1 (MQ=35) aGCCGGGCACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCa < 1:854267/235‑1 (MQ=255) cGGGCACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGGCACCATCAAGGCCGGTGaaa < 1:601978/174‑1 (MQ=18) gggCACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACAccc > 1:657836/1‑191 (MQ=35) gggCACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACAccc < 2:657833/191‑1 (MQ=35) ccccccAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGAt < 1:209889/203‑1 (MQ=38) gggCAACGAAGGCGACCTGGTCAAGGTCACCATCACAGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGcc < 2:486240/220‑1 (MQ=21) cAACGAAGGCGACTTGGTCAAGGTCACCATCACAGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCg < 1:417607/194‑1 (MQ=21) aaCGAAGGCGACTTGGTCAAGGTCACCATCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCAc < 1:240068/236‑1 (MQ=37) aaGGCGACTTGGTCAAGGTCACCATCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTcc < 1:206171/236‑1 (MQ=37) ggCGACTTGGTCAAGGTCACCATCACAGCCGCCCAGACCTCGGTAGCCGAGACCGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTa < 2:116669/167‑1 (MQ=11) ggCGACATGGCCACGGTCACCACCCCAGCCGCCCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTAACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACACCCACGCTGCGCAAg < 2:189929/184‑1 (MQ=11) cGACTTGGTCAAGGTCACCAGCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCtg < 1:273125/236‑1 (MQ=37) aCTTGGTCAAGGTCACCATCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTgcgc > 2:86675/1‑177 (MQ=14) tGGTCAAGGTCACCATCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCTGCTGTGGa > 1:858308/1‑235 (MQ=39) ggtcacggtcacCATCACAGCCGACCAGACCTCGGTGGCTGAGACCGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGAt < 1:89816/181‑1 (MQ=255) gTCACCATCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGAc < 2:588397/234‑1 (MQ=37) cATCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGAt < 2:164030/169‑1 (MQ=40) cATCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCg < 2:356526/234‑1 (MQ=37) tCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAg > 2:689887/1‑136 (MQ=34) cacaGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCtg > 2:644782/1‑215 (MQ=39) gCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCtt < 1:26406/233‑1 (MQ=35) ccGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTcc < 1:9228/206‑1 (MQ=35) ccGACCAGACCTCGGGGGCCGAGAACGGCAAACCGACCTTCAACGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAgc < 2:73067/131‑1 (MQ=255) aGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACAccc > 2:652984/1‑138 (MQ=255) aGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACAccc < 1:652987/138‑1 (MQ=255) aGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCa < 2:597453/123‑1 (MQ=35) ggTGGCTGAGAACGTCAAACCGGCCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTg < 2:413648/235‑1 (MQ=25) ggTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTg < 1:831814/235‑1 (MQ=35) gTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTg < 2:565944/234‑1 (MQ=35) | AAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCACGTCGGTCACCGACGAGCCGGGCACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACAGCCGACCAGACCTCGGTGGCTGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTG > AE015451/207218‑207571 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |