Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	211,003	A→G	8.6%	P5503P (CCA→CCG)	PP_0168 →	putative surface adhesion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	211,003	0	A	G	8.6%	174.9 / 7.5	58	P5503P (CCA→CCG)	PP_0168	putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base A (29/24);  new base G (3/2);  total (32/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.07e-01

TGACGTTTACAACGATGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTAGACGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGGAGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCACTACCGTCACCGACGAGC  >  AE015451/210784‑211235                                                                                                                                                                                                                            |                                                                                                                                                                                                                                         tGACGTTTACAACGCTGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTAGACGCCACTGGTGCGACCTACGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGt                                                                                                                                                                                                                           <  1:883344/235‑1 (MQ=255)         aCAACGATGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTAGACGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATcaac                                                                                                                                                                                                                                    >  2:615235/1‑218 (MQ=40)         aCAACGATGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTAGACGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCAtt                                                                                                                                                                                                                      >  2:645343/1‑232 (MQ=39)         aCAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGATCTTCGAGAACCTGGAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGAcc                                                                                                                                                                                                                  >  1:512754/1‑236 (MQ=255)                   tGGCCAGATCAGCCTGGGCATCAACTCGGCGGTAGACGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTg                                                                                                                                                                                                         <  1:543948/235‑1 (MQ=39)                             aGCCTGGGCATCAACTCGGCGGTAGACGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGc                                                                                                                                                                                                           >  1:891694/1‑223 (MQ=40)                                  gggCATCAACTCGGCGGTAGACGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCACCAACCACAGTGCCTTGACCTTCAc                                                                                                                                                                                                             >  1:53780/1‑216 (MQ=11)                                  gggCATCAACTCGGCGGTAGACGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAAcc                                                                                                                                                                                                                                >  1:283937/1‑197 (MQ=40)                                  gggCATCAACTCGGCGGTAGACGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCg                                                                                                                                                                                         >  1:672484/1‑236 (MQ=255)                                              ggcggTAGACGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTg                                                                                                                                                                              <  1:645346/235‑1 (MQ=37)                                                         ccACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACgg                                                                                                                                                                   >  1:651067/1‑235 (MQ=35)                                                               gTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGt                                                                                                                                                             <  1:744559/235‑1 (MQ=21)                                                                  cGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGt                                                                                                                                                             <  2:497451/232‑1 (MQ=35)                                                                     ccTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTc                                                                                                                                                                                                               >  2:668025/1‑179 (MQ=37)                                                                     ccTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTc                                                                                                                                                                                                               <  1:668028/179‑1 (MQ=35)                                                                       ttCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTg                                                                                                                                                                              <  2:672481/210‑1 (MQ=35)                                                                        tCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGAAGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACt                                                                                                                                                      <  2:509372/233‑1 (MQ=21)                                                                           agaACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGAc                                                                                                                                                                                           <  1:827949/193‑1 (MQ=12)                                                                           agaACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGAc                                                                                                                                                                                           >  2:827946/1‑193 (MQ=9)                                                                             aaCCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCg                                                                                                                                               <  1:11857/235‑1 (MQ=33)                                                                             aaCCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCg                                                                                                                                               <  1:29746/235‑1 (MQ=33)                                                                                  gCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACgg                                                                                                                                                                   >  2:574577/1‑210 (MQ=34)                                                                                     aCTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCaa                                                                                                                                                                      >  1:109675/1‑204 (MQ=34)                                                                                                    ttCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGa                                                                                                                                  <  2:400673/225‑1 (MQ=34)                                                                                                    ttCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGt                                                                                                                            >  2:600342/1‑231 (MQ=35)                                                                                                        gTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCg                                                                                                                                                                                                     <  1:460451/154‑1 (MQ=35)                                                                                                           cAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTa                                                                                                                         >  2:835383/1‑227 (MQ=35)                                                                                                                   cgccaccacCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGc                                                                                                                                                                                                                         <  1:738595/120‑1 (MQ=38)                                                                                                                      caccacCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCt                                                                                                      >  2:867169/1‑235 (MQ=37)                                                                                                                            cgacgaAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGa                                                                                               >  1:647277/1‑236 (MQ=39)                                                                                                                             gacgaAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCg                                                                                                                                               <  2:18968/187‑1 (MQ=35)                                                                                                                                      gtggCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCgg                                                                                                                                   >  2:650271/1‑190 (MQ=35)                                                                                                                                      gtggCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCgg                                                                                                                                   <  1:650274/190‑1 (MQ=35)                                                                                                                                         gCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATcaacaa                                                                                                                                                                                                                                  <  1:338697/92‑1 (MQ=40)                                                                                                                                         gCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATcaacaa                                                                                                                                                                                                                                  >  2:338694/1‑92 (MQ=40)                                                                                                                                          ccAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGCAAcc                                                                                 >  1:577907/1‑236 (MQ=38)                                                                                                                                            aaGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCg                                                                                                                                    <  1:688447/183‑1 (MQ=35)                                                                                                                                                 gACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGTCCAACg                                                                                                                                                                    >  2:305470/1‑146 (MQ=37)                                                                                                                                                       gACCCCTTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGt                                                                                                                                                             <  2:862444/147‑1 (MQ=21)                                                                                                                                                           ccGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGcccc                                                                                                                                     >  1:313167/1‑167 (MQ=33)                                                                                                                                                           ccGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGAAGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGcccc                                                                                                                                     >  1:621545/1‑167 (MQ=21)                                                                                                                                                             gTCCGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGcccc                                                                                                                                     <  2:109674/165‑1 (MQ=18)                                                                                                                                                                      cGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGAt                                                                                        <  2:732343/201‑1 (MQ=38)                                                                                                                                                                            cggcgAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCTACACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGGAGGTGCGGACGTCGGTAAGTTCGAGc                                                  <  2:512751/233‑1 (MQ=37)                                                                                                                                                                             ggcgAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGGAGGTGCGGACGTCGGTAAGTTCGAGCAACt                                              >  1:786521/1‑236 (MQ=38)                                                                                                                                                                                     gcccTACACCCTCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGGAGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTgg                                      <  2:729726/234‑1 (MQ=38)                                                                                                                                                                                          acacCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTggg                                                                                                                                                          >  1:56062/1‑115 (MQ=32)                                                                                                                                                                                                tCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTCACTGCCCCGGACAACGTCTAc                                                                                                                        >  2:212242/1‑143 (MQ=17)                                                                                                                                                                                                   cGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGCAAcc                                                                                 >  2:901775/1‑179 (MQ=37)                                                                                                                                                                                                    gCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGGAGGTCCGGACGTCGGTAAGTTCg                                                     >  1:325387/1‑206 (MQ=25)                                                                                                                                                                                                     cTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGGAGGTGCGGACGTCGGTCAGTTCAAGCa                                                 >  2:431510/1‑209 (MQ=25)                                                                                                                                                                                                     cTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGGAGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAg                      >  1:148930/1‑236 (MQ=37)                                                                                                                                                                                                           aacaaAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGGAGGTGCg                                                                     >  1:599857/1‑183 (MQ=37)                                                                                                                                                                                                           aacaaAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGGAGGTGCg                                                                     <  2:599854/183‑1 (MQ=37)                                                                                                                                                                                                           aacaaAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGGAGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTc                        >  2:793848/1‑228 (MQ=38)                                                                                                                                                                                                                aGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGGAGGTGCGGACGTCGGTAAGt                                                        >  2:636806/1‑191 (MQ=17)                                                                                                                                                                                                                aGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGGAGGTGCGGACGTCGGTAAGt                                                        <  1:636809/191‑1 (MQ=17)                                                                                                                                                                                                                          ccAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGGAGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCACTACCGTCACCGACGAGc  <  1:600345/235‑1 (MQ=39)                                                                                                                                                                                                                            |                                                                                                                                                                                                                                         TGACGTTTACAACGATGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTAGACGCCACTGGTGCGACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGGAGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCACTACCGTCACCGACGAGC  >  AE015451/210784‑211235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑