Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AE015451 212,542 G→C 6.9% T6016T (ACG→ACC)  PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451212,5420GC 6.9% 204.0 / 2.8 69T6016T (ACG→ACC) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base G (39/25);  new base C (3/2);  total (42/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGACCAACAAAGACGGTCTGCCGATTAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTG  >  AE015451/212315‑212777
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           
cTGACCAACAAAGACGGTCTGCCGATTAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCa                                                                                                                                                                                                                                      >  1:389406/1‑235 (MQ=35)
    ccAACAAAGACGGTCTGCCGATTAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCAc                                                                                                                                                                                                                                  >  1:877426/1‑235 (MQ=37)
      aacaaAGACGGTCTGCCGATTAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCTTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGt                                                                                                                                                                                                                                >  2:486750/1‑235 (MQ=37)
       acaaAGACGGTCTGCCGATTAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGATCGAGCAACTGACGCTGGACAAGACGCCGGt                                                                                                                                                                                                                                        >  1:274423/1‑226 (MQ=25)
                   tGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACcgac                                                                                                                                                                                                                     >  2:887775/1‑233 (MQ=11)
                     ccGATTTACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGc                                                                                                                                                                                                                 >  1:662883/1‑235 (MQ=21)
                         ttAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACcga                                                                                                                                                                                                                      <  2:447847/226‑1 (MQ=33)
                                         aTTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTa                                                                                                                                                                                                                          <  2:638547/206‑1 (MQ=33)
                                         aTTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTa                                                                                                                                                                                                                          >  1:638550/1‑206 (MQ=33)
                                         aTTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAAc                                                                                                                                                                                                >  2:340974/1‑232 (MQ=33)
                                              ccTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCggg                                                                                                                                                                                                    >  2:366468/1‑223 (MQ=32)
                                                ttCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGACGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCt                                                                                                                                                                                     >  1:887798/1‑236 (MQ=17)
                                                     ccTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACcgac                                                                                                                                                                                                                     >  2:672787/1‑199 (MQ=33)
                                                     ccTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGt                                                                                                                                                                                  >  1:134260/1‑234 (MQ=32)
                                                     ccTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTc                                                                                                                                                                                 >  1:4070/1‑235 (MQ=32)
                                                     ccTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTc                                                                                                                                                                                 <  1:486753/235‑1 (MQ=33)
                                                     ccTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCa                                                                                                                                                                                >  1:611059/1‑236 (MQ=33)
                                                           cGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCAGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCt                                                                                                                                                                                     >  1:39538/1‑225 (MQ=17)
                                                            gACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGcc                                                                                                                                                                                                                                           <  2:819917/170‑1 (MQ=34)
                                                            gACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGcc                                                                                                                                                                                                                                           >  1:819920/1‑170 (MQ=33)
                                                            gACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGAGGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGGCAGCACGTCGGCTACCGACGAGCCAGGt                                                                                                                                                                                                            >  1:86141/1‑201 (MQ=17)
                                                                 cAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGc                                                                                                                                                                                                                                    >  1:517199/1‑172 (MQ=33)
                                                                 cAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGc                                                                                                                                                                                                                                    <  2:517196/172‑1 (MQ=34)
                                                                  aaaaCCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCAt                                                                                                                                                                      >  2:329075/1‑233 (MQ=33)
                                                                   aaaCCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTa                                                                                                                                                                                                                          <  1:756300/180‑1 (MQ=33)
                                                                       cGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCAccgcc                                                                                                                                                               <  2:320681/235‑1 (MQ=34)
                                                                                    ccGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCgc                                                                                                                                                  >  2:786203/1‑234 (MQ=25)
                                                                                                                cTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCAcc                                                                                                                                                                  >  1:301983/1‑191 (MQ=33)
                                                                                                                 tGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCAcc                                                                                                                     >  1:122636/1‑235 (MQ=39)
                                                                                                                          cccGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACACTGGACAAGACCCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCga                                                                                                                                           >  2:695366/1‑204 (MQ=14)
                                                                                                                            cGGATAACGGCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGAcc                                                                                                                                                      <  2:48988/191‑1 (MQ=25)
                                                                                                                              gATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCaa                                                                                                          >  2:557847/1‑233 (MQ=11)
                                                                                                                                tAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCaa                                                                                                          >  2:477098/1‑231 (MQ=255)
                                                                                                                                      cTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGc                                                                                                                                                                                                        >  1:471326/1‑131 (MQ=34)
                                                                                                                                      cTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCAccgc                                                                                                                                                                >  2:915153/1‑171 (MQ=35)
                                                                                                                                      cTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCAccgc                                                                                                                                                                <  1:915156/171‑1 (MQ=35)
                                                                                                                                      cTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTgg                                                                                               >  1:914817/1‑236 (MQ=255)
                                                                                                                                      cTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCATCGAAGGCGACATGGTCAAGGTCACCATCAcggc                                                                                                                                                                >  1:878671/1‑171 (MQ=12)
                                                                                                                                       tACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCt                                                                                                                          <  1:695369/208‑1 (MQ=40)
                                                                                                                                         cGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGc                                                                                              >  2:841830/1‑234 (MQ=255)
                                                                                                                                             ggCAGCAACGCCCCCGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGGACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCAccgcc                                                                                                                                                               <  2:348874/165‑1 (MQ=255)
                                                                                                                                                agcaACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACACTGGACAAGACCCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGAc                                                                                       >  2:805734/1‑234 (MQ=37)
                                                                                                                                                 gcaACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCg                                                                                                                                                   >  2:818143/1‑173 (MQ=39)
                                                                                                                                                 ccaACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTAACCATCACCGCCGAc                                                                                                                                                            >  2:343348/2‑164 (MQ=25)
                                                                                                                                                     cGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCg                                                                                <  1:887778/236‑1 (MQ=255)
                                                                                                                                                               gTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCa                                                                                                                                                          >  1:662823/1‑152 (MQ=14)
                                                                                                                                                               gTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCGGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCa                                                                                                                                                          <  2:662820/152‑1 (MQ=9)
                                                                                                                                                                tGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGcc                                                                                                    <  2:400003/205‑1 (MQ=255)
                                                                                                                                                                        aTCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCGGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTc                                                                                                                                    <  2:134259/165‑1 (MQ=255)
                                                                                                                                                                        aTCGATACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAg                                                                      <  2:689792/227‑1 (MQ=255)
                                                                                                                                                                          cGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGc                                                                                                                                                                                                                                    <  1:319889/67‑1 (MQ=255)
                                                                                                                                                                          cGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGc                                                                                                                                                                                                                                    >  2:319886/1‑67 (MQ=255)
                                                                                                                                                                          cGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGcc                                                            >  1:708447/1‑235 (MQ=255)
                                                                                                                                                                          cGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACACTGGACAAGACCCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGc                                                             >  1:122762/1‑234 (MQ=12)
                                                                                                                                                                                 gTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAgg                                                        >  2:578712/1‑232 (MQ=255)
                                                                                                                                                                                     gCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCa                                                          >  2:531939/1‑226 (MQ=255)
                                                                                                                                                                                       ggTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGAc                                                                                                                                                       >  2:13859/1‑131 (MQ=34)
                                                                                                                                                                                          gCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATc                                                >  1:75275/1‑231 (MQ=37)
                                                                                                                                                                                                    gCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAAcc                                 <  1:663706/236‑1 (MQ=37)
                                                                                                                                                                                                        gTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCa                                <  2:914814/233‑1 (MQ=39)
                                                                                                                                                                                                        gTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAgcg                             <  1:721348/236‑1 (MQ=37)
                                                                                                                                                                                                        gTTCGAGCAACTGACACTGGACAAGACCCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAgc                              <  1:805737/235‑1 (MQ=37)
                                                                                                                                                                                                         ttCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAgc                              <  2:877423/234‑1 (MQ=37)
                                                                                                                                                                                                            gAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAgcgcg                           <  1:366471/234‑1 (MQ=37)
                                                                                                                                                                                                                   tGACGCTCGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCAGGTACCCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTg                                                                              <  2:906590/176‑1 (MQ=9)
                                                                                                                                                                                                                      cGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGGACACCCa                >  1:164784/1‑235 (MQ=17)
                                                                                                                                                                                                                         tGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCa                                                          <  2:389403/190‑1 (MQ=33)
                                                                                                                                                                                                                           gACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTgc          <  1:672790/236‑1 (MQ=33)
                                                                                                                                                                                                                              aaGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTgcgc        <  1:397007/235‑1 (MQ=32)
                                                                                                                                                                                                                                   gCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGtg  <  1:578715/236‑1 (MQ=32)
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           
CTGACCAACAAAGACGGTCTGCCGATTAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTG  >  AE015451/212315‑212777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑