| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 1,499,497:1 | +C | 84.4% | intergenic (+74/‑90) | trpS → / → zapE | Tryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 1,499,497 | 1 | . | C | 84.4% | 180.8 / 32.6 | 64 | intergenic (+74/‑90) | trpS/zapE | Tryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base . (3/7); new base C (17/37); total (20/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01 | |||||||||||
TGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTG‑T‑T‑T‑CGGGCAC‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCGGG > AE015451/1499273‑1499720 | tGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAc‑g‑c < 1:1458834/236‑1 (MQ=255) ggcGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAc‑g‑cccgctc < 1:969167/233‑6 (MQ=39) cGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑t‑tcgcgggc < 1:528856/216‑8 (MQ=255) gCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACt < 2:586432/234‑1 (MQ=255) gCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCAtt < 1:1397065/235‑1 (MQ=38) gAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGc < 1:881046/235‑1 (MQ=255) gCTTCGGCCTGTGGCGCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑t‑tcgcgggc < 1:1406763/200‑8 (MQ=255) cgtCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑t‑tcgcg < 2:837791/194‑5 (MQ=255) ttCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCg > 1:860361/1‑232 (MQ=255) tCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc < 1:976544/235‑1 (MQ=39) ggCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTAt < 1:683124/236‑1 (MQ=255) ccTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGAc < 1:574378/211‑1 (MQ=255) tgtgGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAACCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCgg > 2:814730/1‑226 (MQ=38) cAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTg < 2:1487062/204‑1 (MQ=37) aTGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑t‑tcgcgggc < 2:1302827/182‑8 (MQ=255) tGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAc‑g‑c < 1:394207/185‑1 (MQ=255) aGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCcac < 2:870331/190‑1 (MQ=37) aCGGGCCGCAGTGCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCgg < 2:293820/199‑1 (MQ=37) ttCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAt < 1:917024/236‑1 (MQ=255) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 1:920308/236‑1 (MQ=255) aCAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCACAC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 1:773992/235‑1 (MQ=39) cAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:860359/233‑1 (MQ=255) aaCGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:1291383/232‑1 (MQ=255) aaGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTAt > 2:1323466/1‑181 (MQ=37) aaGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAAc > 2:1323467/1‑219 (MQ=255) cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACg < 1:1440888/236‑1 (MQ=255) gTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAt < 1:573551/217‑1 (MQ=38) tGCGGCCATTCAGAGCCTGAGCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAATGAATCATGACTCCCTTAGAACGCTATc < 1:919412/236‑1 (MQ=38) gCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 2:1046267/222‑1 (MQ=38) gagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTa < 2:1306669/148‑1 (MQ=21) cTGAGCGAAGCCTGGGCACCACAGCAGGACTGAGGCTGCCAAGCGGTGGGAGCGGGCGTGCCCGCGAAACGGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑t‑tcgcg < 2:216563/111‑5 (MQ=21) aaCGAAGCCTGGGCACCACAGCAGGAGTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCgg < 2:21787/146‑1 (MQ=21) cGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGGG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCCTTAAGCGGGCCCATCGCTACAGTGACGGcc > 2:62183/1‑166 (MQ=25) gaGCCTTGGCACCACAGCAGGACTGGGGCTGCCAAGCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGa < 2:119366/158‑1 (MQ=21) cTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:734779/188‑1 (MQ=37) caccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttg > 1:1330519/1‑164 (MQ=37) acaGCAGGACTGAGGCTGCCAAACGGGGGGGGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCCCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:330601/179‑1 (MQ=37) cagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt > 2:610387/1‑158 (MQ=25) cagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGGTTTCTGTTGCCTCg > 2:1076065/1‑164 (MQ=25) ggACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGACACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 1:609297/184‑1 (MQ=37) cggAGGCTGCCAAACGGTGGGGGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:1142031/168‑1 (MQ=25) cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACgcggcg > 2:543788/1‑226 (MQ=38) cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGGG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGc > 2:272384/1‑123 (MQ=18) aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt > 2:794218/1‑160 (MQ=25) aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 1:794220/160‑1 (MQ=37) aaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 1:1404561/159‑1 (MQ=25) aaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt > 2:1404558/1‑159 (MQ=25) gggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACg < 2:1150297/162‑1 (MQ=37) gggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATg < 2:1206874/148‑1 (MQ=25) gCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttg < 2:1474836/125‑1 (MQ=25) gCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAc‑g‑c > 2:1112848/1‑59 (MQ=1) cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCatg < 1:1385996/223‑3 (MQ=39) cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAgc < 1:843696/229‑1 (MQ=39) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGAT‑C‑G‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCg > 1:101769/1‑112 (MQ=21) gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCTTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCa < 2:1455556/200‑1 (MQ=39) gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCAcc < 2:594723/220‑1 (MQ=255) gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCa > 1:1455559/1‑200 (MQ=39) aaaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCACACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCACGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc < 1:856896/154‑1 (MQ=37) aaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 2:542395/143‑1 (MQ=37) cgccgcGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 2:563559/137‑1 (MQ=37) gccgcGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAc > 1:1058418/1‑181 (MQ=38) cgGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCAcgc < 1:497065/236‑1 (MQ=255) atggcaccGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGaa < 1:543790/229‑1 (MQ=255) gCTTTGCCGGTG‑TCC‑G‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATg < 2:345622/160‑1 (MQ=255) cTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACg > 1:1100902/1‑121 (MQ=38) cTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCg > 2:795061/1‑184 (MQ=255) ttGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 2:1019236/1‑137 (MQ=39) ttGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCggg > 1:1117542/1‑236 (MQ=255) ccGGTG‑T‑TCG‑CGGGCAC‑GCCCTCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCCTCCATGACGCGGTGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTgg < 1:166898/208‑1 (MQ=255) | TGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTG‑T‑T‑T‑CGGGCAC‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCGGG > AE015451/1499273‑1499720 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |