| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 2,087,315:1 | +C | 6.2% | intergenic (+128/+790) | PP_1865 → / ← aroH | transposase/Phospho‑2‑dehydro‑3‑deoxyheptonate aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 2,087,315 | 1 | . | C | 6.2% | 341.9 / 14.8 | 81 | intergenic (+128/+790) | PP_1865/aroH | transposase/Phospho‑2‑dehydro‑3‑deoxyheptonate aldolase |
| Reads supporting (aligned to +/- strand): ref base . (26/50); new base C (3/2); total (29/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.60e-01 | |||||||||||
GAGGCCCCAAGGTGCTCAAAGCGAAGGCCTGGGTGCAAGGGACGGCTGTACATGCTCATGTTTCGACGGATCGGGTCATCAAAGCCGCCAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATG > AE015451/2087081‑2087547 | gAGGCCCCAAGGTGCTCAAAGCGAAGGCCTGGGTGCAAGGGACGGCTGTACATGCTCATGTTTCGACGGATCGGGTCATCAAAGCCGCCAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCaa < 1:198469/236‑2 (MQ=255) aaGGTGCTCAAAGCGAAGGCCTGGGTGCAAGGGACGGCTGTACATGCTCATGTTTCGACGGATCGGGTCATCAAAGCCGCCAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑AGCGCt < 2:851572/233‑1 (MQ=255) tGCTCAAAGCGAAGGCCTGGGTGCAAGGGACGGCTGTACATGCTCATGTTTCGACGGATCGGGTCATCAAAGCCGCCAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGcc < 1:1379413/236‑1 (MQ=255) cTCAAAGCGAAGGCCTGGGTGCAAGGGACGGCTGTACATGCTCATGTTTCGACGGATCGGGTCATCAAAGCCGCAAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGGCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGcc < 1:516744/234‑1 (MQ=255) tCAAAGCGAAGGCCTGGGTGCAAGGGACGGCTGTACATGCTCATGTTTCGACGGATCGGGTCATCAAAGCCGCCAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCa < 2:1000174/229‑1 (MQ=255) aaaGCTAAGGCCTGGGTGCAAGGGACGGCTGTACATGCTCATGTTTCGACGGATCGGGTCATCAAAGCCGCCAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTGCGCTACGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGGACCGCGCTg > 1:1354783/1‑226 (MQ=255) aGCGAAGGCCTGGGTGCAAGGGACGGCTGTACATGCTCAGGTTTCGACGGATCGGGTCATCAAAGCCGCCAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAACACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCccatcca < 1:385451/236‑1 (MQ=255) cgcaGGCCTGGGTGCAAGGGACGGCTGTACATGCTCATGTTTCGACGGATCGGGTCATCAAAGCCGCCAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAg < 1:489447/221‑1 (MQ=255) cGAAGGCCTGGGTGCAAGGGACGGCTGTACATGCTCATGTTTCGACGGATCGGGTCATCAAAGCCGCCAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCt < 1:960169/220‑1 (MQ=255) cGAAGGCCTGGGTGCAAGGGACGGCTGTACATGCTCATGTTTCGACGGATCGGGTCATCAAAGCCGCCAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATg < 1:872793/236‑1 (MQ=255) ccTGGGTGCAAGGGACGGCTGTACATGCTCATGTTTCGACGGATCGGGTCATCAAAGCCGCCAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGcc > 1:641280/1‑235 (MQ=255) aGGGACGGCTGTACATGCTCATGTTTCGACGGATCGGGTCATCAAAGCCGCCAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTGCGCTACGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGGACCGCGCTGCAGGcccc < 2:1354780/214‑1 (MQ=255) aGGGACGGCTGTACATGCTCATGTTTCGACGGATCGGGTCATCAAAGCCGCCAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATgg < 2:616755/221‑1 (MQ=255) cGGCTGTACATGCTCATGTTTCGACGGATCGGGTCATCAAAGCCGCCAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑cgcg < 2:1169820/197‑1 (MQ=255) cTGTACATGCTCATGTTTCGACGGATCGGGTCATCAAAGCCGCCAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTg > 1:944177/1‑197 (MQ=255) aagagacagCTCATGTTTCGACGGATCGGGTCATCAAAGCCGCCAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCC‑‑CCATGGGGCCT‑CAGCGCTTGACGGGCATcc < 1:643623/227‑1 (MQ=255) tGTACATGCTCATGTTTCGACGGATCGGGTCATCAAAGCCGCCAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCccatc > 2:631832/1‑207 (MQ=255) tGTACATGCTCATGTTTCGACGGATCGGGTCATCAAAGCCGCCAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATc > 1:649691/1‑235 (MQ=255) aTGCTCATGTTTCGACGGATCGGGTCATCAAAGCCGCCAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCTGCAGCGCTTg < 1:194339/222‑1 (MQ=255) cATGTTTCGACGGATCGGATCATCAAAGCCGCCAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGAc < 1:1507319/218‑1 (MQ=255) tttCGACGGATCGGGTCATCAAAGCCGCCAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCa < 2:1387668/175‑1 (MQ=255) gATCGGGTCATCAAAGCCGCCAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGGCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCC‑‑CCATGGGGCCT‑CAgcga > 2:643621/1‑198 (MQ=255) gggTCATCAAAGCCGCCAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGa > 2:1438032/1‑201 (MQ=255) aaGCCGCCAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGccc > 2:535348/1‑207 (MQ=255) gccgccAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCAt < 1:435470/201‑1 (MQ=255) caaaaccaaaaGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTC < 2:980576‑M1/212‑1 (MQ=255) ccTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCT < 1:535350‑M1/236‑1 (MQ=255) ccTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATC < 1:1091064‑M1/235‑1 (MQ=255) cTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCACAAAGGAACCAAAAACCGGCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATC < 1:310230‑M1/234‑1 (MQ=255) cTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCT < 1:1438035‑M1/235‑1 (MQ=255) gAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCccat < 1:96598/147‑1 (MQ=255) gAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGT < 1:1320986‑M1/236‑1 (MQ=255) aatggATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑cgcg < 2:1326083/129‑1 (MQ=255) aaGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGC < 2:593038‑M1/232‑1 (MQ=255) aaGGGATGGCCCTTACGGCGGGTCCC‑TTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCCACGCTGCGCCCCGGGGTCCCCCCGCTCCCGCCTTGCTCCCGGGAGCA‑CGCGCTGc > 2:1006056/1‑135 (MQ=17) tGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCAt < 1:1363022/167‑1 (MQ=255) cTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGT < 1:1247913‑M1/223‑1 (MQ=255) aCGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATc < 2:944175/160‑1 (MQ=255) cggcggGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACcgcgc < 2:649689/236‑1 (MQ=255) gTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATc > 2:880376/1‑153 (MQ=255) cTTGTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTtgcct < 1:675391‑M1/214‑6 (MQ=255) tttGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAg > 1:793884/1‑110 (MQ=255) tttGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAg < 2:793882/110‑1 (MQ=255) ttGTCGTGGCAAACAGGAACCAAACACCGTCGGCCCCCAGCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTC < 2:360920‑M1/180‑1 (MQ=255) tCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTtgcctgaactacc < 2:773725‑M1/215‑14 (MQ=255) gTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTa < 1:553017/222‑1 (MQ=255) gTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTa > 2:553015/1‑222 (MQ=255) gTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCt > 2:528756/1‑220 (MQ=255) gTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCC‑CAGCCCTTGACGGGCATCCATGCCCGTCATctccccc > 2:1259000/1‑157 (MQ=255) gtggccaaagtaaccAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGC < 1:717380‑M1/191‑1 (MQ=255) aaaGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTC < 2:875561‑M1/168‑1 (MQ=255) aaaaaCCGTCGACTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGCTACCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCCCTGCAGCCCCCATCCATGGTCCCT‑CAGCGCTCGACCGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCT > 1:1191534‑M1/1‑164 (MQ=255) aaaaCCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTC < 1:411818‑M1/142‑1 (MQ=255) aaaaCCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTC < 2:1116323‑M1/142‑1 (MQ=255) aaaaCCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTC > 1:1116326‑M1/1‑142 (MQ=255) aaaaCCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTC > 2:411816‑M1/1‑142 (MQ=255) cGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCTATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGa < 1:798749/235‑1 (MQ=255) cGTCCGCTCCCATCCTCCCGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTtgcctgaacta < 1:265693‑M1/189‑12 (MQ=255) gCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCAt > 1:456530/1‑125 (MQ=255) gCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCAt < 2:456528/125‑1 (MQ=255) gCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCgag > 2:991255/1‑215 (MQ=255) cTCCCATCATCCGGCCCCGACGCTGCGCTCCGGGGTCCCCGCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATC < 2:59004‑M1/167‑1 (MQ=255) tACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCg < 1:727073/236‑1 (MQ=255) tGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCgcgcgc < 2:153974/168‑1 (MQ=255) cTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTtgcctgaactaccgcgcacttagaagcacgatcaaaagcgaaaagcgcttagcgccgtacctgcttgagggtttcggcaatcaggaacgc > 1:1448799‑M1/1‑145 (MQ=255) cTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATCCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGa > 2:348332/1‑153 (MQ=255) tCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCc > 2:167639‑M1/1‑106 (MQ=255) ccGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTtgcctgaactaccgcgcacttagaagcacgatcaaaagcgaaaagcgcttagcgccgtacctgcttgagggtttcggcaatcaggaacgcc > 2:761269‑M1/1‑143 (MQ=255) ggTCCCCTCGCTCCGGCCTTGCTTCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTtgcctgaactaccgcgcacttagaagcacgatcaaaagcgaaaagcgcttagcgccgtacctgcttgagggtttcggcaatcaggaacgccaact > 2:1398293‑M1/1‑139 (MQ=255) ggTCCCCTCACTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCgg > 2:795885/1‑194 (MQ=255) ccccTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGt < 1:631834/235‑1 (MQ=255) ccTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTc > 1:860260/1‑234 (MQ=255) gCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCg < 1:1210676/132‑1 (MQ=255) gCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTg < 1:1349309/235‑1 (MQ=255) cTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGt < 1:1158192/228‑1 (MQ=255) ccGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGaaa > 2:1058118/1‑234 (MQ=255) cGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGc < 1:555403/203‑1 (MQ=255) cGGCCGTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTtgcctgaactaccgcgcacttagaagcacgatcaaaagcgaaaagc < 2:477221‑M1/172‑47 (MQ=255) gCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTtgcctgaactaccgcgcacttagaagcaagatcaaaagcgaaaagcgctt > 2:1286944‑M1/1‑124 (MQ=255) tGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCT > 2:178539‑M1/1‑117 (MQ=255) tGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCT < 1:178540‑M1/117‑1 (MQ=255) tCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGt < 1:1051554/216‑1 (MQ=255) ccGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATaa < 1:917655/225‑1 (MQ=255) gCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTtgcctgaactaccgcgcacttagaagcacgatcaaaagcgaaaagcgcttagcgccgtacctgcttgagggtttcggcaatcaggaacgccaactccagcgactgatcggcattcatccgcggatc < 1:1398296‑M1/235‑127 (MQ=255) cA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTtgcctgaactaccgcgcacttagaagcacgatcaaaagcgaaaagcgcttagcgccgtacctgcttgaggg < 1:144538‑M1/179‑72 (MQ=255) | GAGGCCCCAAGGTGCTCAAAGCGAAGGCCTGGGTGCAAGGGACGGCTGTACATGCTCATGTTTCGACGGATCGGGTCATCAAAGCCGCCAAAACCAAAAGACCTTGAAAGGGATGGCCCTTACGGCGGGTCCCTTTTTGTCGTGGCAAAAAGGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCCATGGGGCCT‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATG > AE015451/2087081‑2087547 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |