New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	250227 =	59 (0.690)	3 (0.040)	3/336	NT	5.5%	coding (976/1347 nt)	nfeD	putative membrane bound peptidase, nefD
	?	AE015451	250271 =	47 (0.580)					coding (932/1347 nt)	nfeD	putative membrane bound peptidase, nefD

TCACCAACCCCAGCGTAGCGCTGATCCTGATCATGATCGGCGTTTACGGCCTGTTGTTCGAATTCATGAACCCAGGTTCTGCGGTGGGCGGTGTGGTCGGTGGTATCTGCCTGCTGCTGGCGCTGTATGCGCTGCAGCTGTTGCCGGTGAGTTTTGCCGGGGTGGCGCTGATCCTGCTTGGCATCACCTTCATGATCGCCGAAGCCTTCTTGCCCAGTTTCGGCGTGGTCGGCTTCGGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AE015451/250466‑250227 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcttcggcgATCATGAAGGTGATGCCAAGCAGGATCAGCGCCACCCCGGCAAAACTCACCGGCAACAGCTGCAGCGCATACAGCGCCAGCAGCAGGCAGATACCACCGACCACACCGCCCACCGCAGAACCTGGGTTCATGAATTCGAACAACAGGCCGTAAACGCCGATCATGATCAGGATCAGCGCTACGCTGGGGTTGGTGATGACCGCCAGAAACCGTGTGCGCCAGTCCGGCAGA  >  AE015451/250271‑250501                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         TCACCAACCCCAGCGTAGCGCTGATCCTGATCATGATCGGCGTTTACGGCCTGTTGTTCGAATTCATGAACCCAGGTTCTGCGGTGGGCGGTGTGGTCGGTGGTATCTGCCTGCTGCTGGCGCTGTATGCGCTGCAGCTGTTGCCGGTGAGTTTTGCCGGGGTGGCGCTGATCCTGCTTGGCATCACCTTCATGATCGCCGAAGCCTTCTTGCCCAGTTTCGGCGTGGTCGGCTTC                                                                                                                                                                                                                                             >  1:559380/1‑236  CACCAACCCCAGCGTAGCGCTGACCCTGATCATGATCGGCGTTTACGGCCTGTTGTTCGAATTCATGAACCCAGGTTCTGCGGTGGGCGGTGTGGTCGGTGGTATCTGCCTGCTGCTGGCGCTGTATGCGCTGCAGCTGTTGCCGGTGAGTTTTGCCGGGGTGGCGCTGATCCTGCTTGGCATCACCTTCATGATCGCCGAAGCCTTCTTGCCCAGTTTCGGCGTGGTCGGCTTCG                                                                                                                                                                                                                                            <  1:831912/236‑1                      TGATCCTGATCATGATCGGCGTTTACGGCCTGTTGTTCGAATTCATGAACCCAGGTTCTGCGGTGGGCGGTGTGGTCGGTGGTATCTGCCTGCTGCTGGCGCTGTATGCGCTGCAGCTGTTGCCGGTGAGTTTTGCCGGGGTGGCGCTGATCCTGCTTGGCATCACCTTCATGATCGCCGAAGCCTTCTTGCCCAGTTTCGGCGTGGTCGG                                                                                                                                                                                                                                                 >  2:502651/1‑211                                                                                                                                                                                                        CGAAGCCTTCTTGCCCAGTTTCGGCGTGGTCGGCTTCGGCGATCATGAAGGTGATGCCAAGCAGGATCAGCGCCACCCCGGCAAAACTCACCGGCAACAGCTGCAGCGCATACAGCGCCAG                                                                                                                                                         <  1:118622/121‑1                                                                                                                                                                                                        CGAAGCCTTCTTGCCCAGTTTCGGCGTGGTCGGCTTCGGCGATCATGAAGGTGATGCCAAGCAGGATCAGCGCCACCCCGGCAAAACTCACCGGCAACAGCTGCAGCGCATACAGCGCCAG                                                                                                                                                         >  2:118621/1‑121                                                                                                                                                                                                                TCTTGCCCAGTTTCGGCGTGGTCCGCTTCGGCGATCATGAAGGTGATCCCAAGCAGGATCAGCGCCACCCCGGCAAAACTCACCGGCAACAG                                                                                                                                                                              <  2:206892/92‑1                                                                                                                                                                                                                                          TTCGGCGATCATGAAGGTGATGCCAAGCAGGATCAGCGCCACCCCGGCAAAACTCACCGGCAACAGCTGCAGCGCATACAGCGCCAGCAGCAGGCAGATACCACCGACCACACCGCCCACCGCAGAACCTGGGTTCATGAATTCGAACAACAGGCCGTAAACGCCG                                                                          <  1:1510458/166‑1                                                                                                                                                                                                                                            CGGCGATCATGAAGGTGATGCCAAGCAGGATCAGCGCCACCCCGGCAAAACTCACCGGCAACAGCTGCAGCGCATACAGCGCCAGCAGCAGGCAGATACCACCGACCACACCGCCCACCGCAGAACCTGGGTTCATGAATTCGAACAACAGGCCGTAAACGCCG                                                                          >  1:705320/1‑164                                                                                                                                                                                                                                            CGGCGATCATGAAGGTGATGCCAAGCAGGATCAGCGCCACCCCGGCAAAACTCACCGGCAACAGCTGCAGCGCATACAGCGCCAGCAGCAGGCAGATACCACCGACCACACCGCCCACCGCAGAACCTGGGTTCATGAATTCGAACAACAGGCCGTAAACGCCG                                                                          <  2:705318/164‑1                                                                                                                                                                                                                                             GGCGATCATGAAGGTGATGCCAAGCAGGATCAGCGCCACCCCGGCAAAACTCACCGGCAACAGCTGCAGCGCATACAGCGCCAGCAGCAGGCAGATACCACCGACCACACCGCCCACCGCAGAACCTGGGTTCATGAATTCGAACAACAGGCCGTAAACGCCGATCATGATCAGGATCAGCGCTACGCTGGGGTTGGTGATGACCGCCAGAAACCGTGTGCGCCAGTCCGGCAGA  <  1:818180/235‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         TCACCAACCCCAGCGTAGCGCTGATCCTGATCATGATCGGCGTTTACGGCCTGTTGTTCGAATTCATGAACCCAGGTTCTGCGGTGGGCGGTGTGGTCGGTGGTATCTGCCTGCTGCTGGCGCTGTATGCGCTGCAGCTGTTGCCGGTGAGTTTTGCCGGGGTGGCGCTGATCCTGCTTGGCATCACCTTCATGATCGCCGAAGCCTTCTTGCCCAGTTTCGGCGTGGTCGGCTTCGGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AE015451/250466‑250227 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcttcggcgATCATGAAGGTGATGCCAAGCAGGATCAGCGCCACCCCGGCAAAACTCACCGGCAACAGCTGCAGCGCATACAGCGCCAGCAGCAGGCAGATACCACCGACCACACCGCCCACCGCAGAACCTGGGTTCATGAATTCGAACAACAGGCCGTAAACGCCGATCATGATCAGGATCAGCGCTACGCTGGGGTTGGTGATGACCGCCAGAAACCGTGTGCGCCAGTCCGGCAGA  >  AE015451/250271‑250501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.