New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	2794992 =	78 (0.910)	5 (0.060)	5/340	NT	6.6%	intergenic (+100/+12)	PP_2447/puuB	conserved exported protein of unknown function/gamma‑glutamylputrescine oxidase
	?	AE015451	2795106 =	67 (0.820)					coding (1182/1284 nt)	puuB	gamma‑glutamylputrescine oxidase

GCCGGATTGGCGACAACATCTACTACTCGCAGGGTTGCTCGGGCCACGGCGTGACCTATACCCACCTGGCGGGCAAGGTGCTGGCCGAGGCCCTGCGCGGCCAGGCAGAGCGTTTCGACGCCTTTGCCGGCCTGCCGCACTACCCGTTCCCGGGTGGCCAGATGCTGCGCGTACCCTTCAGCGCCATCGGTGCCTGGTACTACAGCCTGCGCGATCGCCTGGGCTTCTGATTCAGGCAGGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AE015451/2795233‑2794992 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcaggccGGCAAAGGCGTCGAAACGCTCTGCCTGGCCGCGCAGGGCCTCGGCCAGCACCTTGCCCGCCAGGTGGGTATAGGTCACGCCGTGGCCCGAGCAACCCTGCGAGTAGTAGATGTTGTCGCCAATCCGGCCAACCTGTGGCAGGCGCGACAAGGTCAGCAGGAAGTTACCGGTCCAGGCGTAGTCGATCTTCACGTTCTTCAGCTGCGGGAAGGCCTTGAGCATTTTCGGGCGGAT  >  AE015451/2795106‑2795339                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              GCCGGATTGGCGACAACATCTACTACTCGCAGGGTTGCTCGGGCCACGGCGTGACCTATACCCACCTGGCGGGCAAGGTGCTGGCCGAGGCCCTGCGCGGCCAGGCAGAGCGTTTCGACGCCTTTGCCGGCCTGCCGCACTACCCGTTCCCGGGTGGCCAGATGCTGCGCGTACCCTTCAGCGCCATCGGTGCCTGGTACTCCAGCCTGCGCGATCGCCTGGGCTTCTGATTCAG                                                                                                                                                                                                                                                   >  1:53608/1‑235   CGGATTGGCGACAACATCTACTACTCGCAGGGTTGCTCGGGCCACGGCGTGACCTATACCCACCTGGCGGGCAAGGTGCTGGCCGAGGCCCTGCGCGGCCAGGCAGAGCGTTTCGACGCCTTTGCCGGCCTGCCGCACTACCCGTTCCCGGGTGGCCAGATGCTGCGCGTACCCTTCAGCGCCATCGGTGCCTGGTACTACAGCCTGCGCGATCGCCTGGGCTTCTGATTCAGGCA                                                                                                                                                                                                                                                >  1:924711/1‑236   CGGATTGGCGACAACATCTACTACTCGCAGGGTTGCTCGGGCCACGGCGTGACCTATACCCACCTGGCGGGCAAGGTGCTGGCCGAGGCCCTGCGCGGCCAGGCAGAGCGTTTCGACGCCTTTGCCGGCCTGCCGCACTACCCGTTCCCGGGTGGCCAGATGCTGCGCGTACCCTTCAGCGCCATCGGTGCCTGGTACTACAGCCTGCGCGATCGCCTGGGCTTCTGATTCAGGCA                                                                                                                                                                                                                                                <  1:741479/236‑1      ATTGGCGACAACATCTACTACTCGCAGGGTTGCTCGGGCCACGGCGTGACCTATACCCACCTGGCGGGCAAGGTGCTGGCCGAGGCCCTGCGCGGCCAGGCAGAGCGTTTCGACGCCTTTGCCGGCCTGCCGCACTACCCGTTCCCGGGTGGCCAGATGCTGCGCGTACCCTTCAGCGCCATCGGTGCCTGGTACTACAGCCTGCGCGATCGCCTGGGCTTCTGATTCAGGCAGGC                                                                                                                                                                                                                                             <  1:1175264/236‑1                ACATCTACTACTCGCAGGGTTGCTCGGGCAACGGCGTGACCTATACCCACCTGGCGGGCAAGGTGCTGGCCGAGGCCCTGCGCGGCCAGGCAGAGCGTTTCGACGCCTTTGCCGGCCTGCCGCACTACCCGTTCCCGGGTGGCCAGATGCTGCGCGTACCCTTCAGCGCCATCGGTGCCTGGTACTACAGCCTGCGCGATCGCCTGGGCTTCTGATTCAGG                                                                                                                                                                                                                                                  <  2:595814/221‑1                                  GTTGCTCGGGCCACGGCGTGACCTATACCCACCTGGCGGGCAAGGTGCTGGCCGAGGCCCTGCGCGGCCAGGCAGAGCGTTTCGACGCCTTTGCCGGCCTGCCGCACTACCCGTTCCCGGGTGGCCAGATGCTGCGCGTACCCTTCAGCGCCATCGGTGCCTGGTACTACAGCCTGCGCGATCGCCTGGGCTTCTGATTCAGGCAGGC                                                                                                                                                                                                                                             <  1:1332831/208‑1                                  GTTGCTCGGGCCACGGCGTGACCTATACCCACCTGGCGGGCAAGGTGCTGGCCGAGGCCCTGCGCGGCCAGGCAGAGCGTTTCGACGCCTTTGCCGGCCTGCCGCACTACCCGTTCCCGGGTGGCCAGATGCTGCGCGTACCCTTCAGCGCCATCGGTGCCTGGTACTACAGCCTGCGCGATCGCCTGGGCTTCTGATTCAGGCAGGC                                                                                                                                                                                                                                             >  2:1332828/1‑208                                                        CTATACCCACCTGGCGGGCAAGGTGCTGGCCGAGGCCCTGCGCGGCCAGGCAGAGCGTTTCGACGCCTTTGCCGGCCTGCCGCACTACCCGTTCCCGGGTGGCCAGATGCTGCGCGTACCCTTCAGCGCCATCGGTGCCTGGTACTACAGCCTGCGCGATCGCCTGGGCTTCTGATTCAGGCA                                                                                                                                                                                                                                                >  2:1052932/1‑183                                                                                  TGGCCGAGGCCCTGCGCGGCCAGGCAGAGCGTTTCGACGCCTTTGCCGGCCTGCCGCACTACCCGTTCCCGGGTGGCCAGATGCTGCGCGTACCCTTCAGCGCCATCGGTGCCTGGTACTACAGCCTGCGCGATCGCCTGGGCTTCTGATTCAGGCAGGCCGGCAAAGGCGTCGAA                                                                                                                                                                                                                             <  2:922848/176‑1                                                                                                                GTTTCGACGCCTTTGCCGGCCTGCCGCACTACCCGTTCCCGGGTGGCCAGATGCTGCGCGTACCCTTCAGCGCCATCGGTGCCTGGTACTACAGCCTGCGCGATCGCCTGGGCTTCTGATTCAGGCAGGC                                                                                                                                                                                                                                             <  2:1301841/130‑1                                                                                                                GTTTCGACGCCTTTGCCGGCCTGCCGCACTACCCGTTCCCGGGTGGCCAGATGCTGCGCGTACCCTTCAGCGCCATCGGTGCCTGGTACTACAGCCTGCGCGATCGCCTGGGCTTCTGATTCAGGCAGGC                                                                                                                                                                                                                                             >  1:1301844/1‑130                                                                                                                              GCCGGCCTGCCGCACTACCCGTTCCCGGGTGGCCAGATGCTGCGCGTACCCTTCAGCGCCATCGGTGCCTGGTACTACAGCCTGCGCGATCCCCTGGGCTTCTGATTCAGGCAGGC                                                                                                                                                                                                                                             >  2:49329/1‑116                                                                                                                                                       CGGGTGGCCAGATGCTGCGCGTACCCTTCAGCGCCATCGGTGCCTGGTACTACAGCCTGCGCGATCGCCTGGGCTTCTGATTCAGGCAGGCCGGCAAAGGCGTCGAAACGCTCTGCCTGGCCGCGCAGGGCCTCGGCCAGCACCTTGCCCGCCAGGTGGGTATAGGTCACGCCGTGGCCCGAGCAACCCTGCGAGTA                                                                                                                                   >  1:625383/1‑197                                                                                                                                                                                              GTGCCTGGTACTACAGCCTGCGCGATCGCCTGGGCTTCTGATTCAGGCAGGCCGGCAAAGGCGTCGAAACGCTCTGCCTGGCCGCGCAGGGCCTCGGCCAGCACCTTGCC                                                                                                                                                                                   >  1:579164/1‑110                                                                                                                                                                                              GTGCCTGGTACTACAGCCTGCGCGATCGCCTGGGCTTCTGATTCAGGCAGGCCGGCAAAGGCGTCGAAACGCTCTGCCTGGCCGCGCAGGGCCTCGGCCAGCACCTTGCC                                                                                                                                                                                   <  2:579162/110‑1                                                                                                                                                                                                                 GCGCGATCGCCTGGGCTTCTGATTCAGGCAGGCCGGCAAAGGCGTCGAAACGCTCTGCCTGGCCGCGCAGGGCCTCGGCCAGCACCTTGCCCGCCAGGGGGGTATAGG                                                                                                                                                                  >  2:750148/1‑108                                                                                                                                                                                                                  CGCGATCGCCTGGGCTTCTGATTCAGGCA                                                                                                                                                                                                                                                <  2:1317169/29‑1                                                                                                                                                                                                                                                GCCGGCAAAGGCGTCGAAACGCTCTGCCTGGCCGCGCAGGGCCTCGGCCAGCACCTTGCCCGCCAGGTGGGTATAGGTCACGCCGTGGCCCGAGCAACCCTGCGAGTAGTAGATGTTGTCGCCAATCCGGCCAACCTGTGGCAGGCGCGACAAGGTCAGCAGGAAGTTACCGGTCCAGGCGTAGTCGATCTTCACGTTCTTCAGCTGCGGGAAGGCCTTGAGCATTTTCG         <  1:676447/230‑1                                                                                                                                                                                                                                                 CCGGCAAAGGCGTCGAAACGCTCTGCCTGGCCGCGCAGGGCCTCGGCCAGCACCTTGCCCGCCAGGTGGGTATAGGTCACGCCGTGGCCCGAGCAACCCTGCGAGTAGTAGATGTTGTCGCCAATCCGGCCAACCTGTGGCAGGCGCGACAAGGTCAGCAGGAAGTTACCGGTCCAGGCGTAGTCGATCTTCACGTTCTTCAGCTGCGGGAAGGCCTTGAGCATTTTCGGGCGGAT  <  1:19503/236‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              GCCGGATTGGCGACAACATCTACTACTCGCAGGGTTGCTCGGGCCACGGCGTGACCTATACCCACCTGGCGGGCAAGGTGCTGGCCGAGGCCCTGCGCGGCCAGGCAGAGCGTTTCGACGCCTTTGCCGGCCTGCCGCACTACCCGTTCCCGGGTGGCCAGATGCTGCGCGTACCCTTCAGCGCCATCGGTGCCTGGTACTACAGCCTGCGCGATCGCCTGGGCTTCTGATTCAGGCAGGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AE015451/2795233‑2794992 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcaggccGGCAAAGGCGTCGAAACGCTCTGCCTGGCCGCGCAGGGCCTCGGCCAGCACCTTGCCCGCCAGGTGGGTATAGGTCACGCCGTGGCCCGAGCAACCCTGCGAGTAGTAGATGTTGTCGCCAATCCGGCCAACCTGTGGCAGGCGCGACAAGGTCAGCAGGAAGTTACCGGTCCAGGCGTAGTCGATCTTCACGTTCTTCAGCTGCGGGAAGGCCTTGAGCATTTTCGGGCGGAT  >  AE015451/2795106‑2795339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.