New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	6070767 =	101 (1.170)	3 (0.040)	3/336	NT	3.2%	intergenic (‑123/+40)	PP_5324/phoU	Response regulator/phosphate transport‑associated protein
	?	AE015451	6070789 =	89 (1.100)					intergenic (‑145/+18)	PP_5324/phoU	Response regulator/phosphate transport‑associated protein
Rejected: Frequency below/above cutoff threshold.

GGGTGCTGAGCGTGATCTGGGTGCTGCGCTCGCTGGAGCGTATTGGCGACCATGCGCGCAACATCTCCGAACTGGTGATCTACCTGGTGCGCGGCACCGACGTGCGCCACATGGGCCTCAAGCGCATGACGGCAGAAGTGCAGGGCACTGCTGTCGCCAACGGCGAAAAGGCTAATGTTCCGGGTGAATCTGACGATAAGTAAGGTTGCTCCCGAGCATCGACGCCCGGCCTTGGCCGGGCGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AE015451/6071009‑6070767 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggccgggcgtCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGACAGCAGTGCCCTGCACTTCTGCCGTCATGCGCTTGAGGCCCATGTGGCGCACGTCGGTGCCGCGCACCAGGTAGATCACCAGTTCGGAGATGTTGCGCGCATGGTCGCCAATACGCTCCAGCGAGCGCAGCACCCAGATCACGCTCAGCACCCGCGAGATAGAG  >  AE015451/6070789‑6071020                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             GGGTGCTGAGCGTGATCTGGGTGCTGCGCTCGCTGGAGCGTATTGGCGACCATGCGCGCAACATCTCCGAACTGGTGATCTACCTGGTGCGCGGCACCGACGTGCGCCACATGGGCCTCAAGCGCATGACGGCAGAAGTGCAGGGCACTGCTGTCGCCAACGGCGAAAAGGCTAATGTTCCGGGTGAATCTGACGATAAGTAAGGTTGCTCCCGAGCATCGACGCCCGGCCTTG                                                                                                                                                                                                                                                   >  2:636340/1‑234        GAGCGTGATCTGGGTGCTGCGCTCGCTGGAGCGTATTGGCGACCATGCGCGCAACATCTCCGAACTGGTGATCTACCTGGTGCGCGGCACCGACGTGCGCCACATGGGCCTCAAGCGCATGACGGCAGAAGTGCAGGGCACTGCTGTCGCCAACGGCGAAAAGGCTAATGTTCCGGGTGAATCTGACGATAAGTAAGGTTGCTCCCGAGCATCGACGCCCGGCCTTGGCCGGGC                                                                                                                                                                                                                                            >  2:1475680/1‑234            GTGATCTGGGTGCTGCGCTCGCTGGAGCGTATTGGCGACCATGCGCGCAACATCTCCGAACTGGTGATCTACCTGGTGCGCGGCACCGACGTGCGCCACATGGGCCTCAAGCGCATGACGGCAGAAGTGCAGGGCACTGCTGTCGCCAACGGCGAAAAGGCTAATGTTCCGGGTGAATCTGACGATAAGTAAGGTTGCTCCCGAGCATCGACGCCCGGCCTTGGCCGGGCGTCGA                                                                                                                                                                                                                                       >  1:694964/1‑235                                                        GCGCAACATCTCCGAACTGGTGATCTACCTGGTGCGCGGCACCGACGTGCGCCACATGGGCCTCAAGCGCATGACGGCAGAAGTGCAGGGCACTGCTGTCGCCAACGGCGAAAAGGCTAATGTTCCGGGTGAATCTGACGATAAGTAAGGTTGCTCCCGAGCATCGACGCCCGGCCTTGGCCGG                                                                                                                                                                                                                                              >  2:556398/1‑184                                                                                                       gGCGCCACATGGGCCTCAAGCGCATGACGGCAGAAGGGCAGGGCACTGCTGTCGCCAACGGCGAAAAGGCTAATGTTCCGGGTGAATCTGACGATAAGTAAGGTTGCTCCCGAGCATCGACGCCCGGCCTTGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTC                                                                                                                                                                                                      <  2:165606/176‑1                                                                                                            CACATGGGCCTCAAGCGCATGACGGCAGAAGTGCAGGGCACTGCTGTCGCCAACGGCGAAAAGGCTAATGTTCCGGGTGAATCTGACGATAAGTAAGGTTGCTCCCGAGCATCGACGCCCGGCCTTGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCG                                                                                                                                                                                <  2:694962/194‑1                                                                                                                                                                                     CGGGTGAGTCTGACGATAAGTAGGGTTGCTCCCGGGCATCGACGCCCGGCCTTGGCCGGGCGTCGATGCTCGGGAGCAA                                                                                                                                                                                                                          <  2:52289/79‑1                                                                                                                                                                                                                                               GGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGACAGCAGTGCCCTGCACTTCTGCCGTCATGCGCTTGAGGCCCATGTGGCGCACGTCGGTGCCGCGCACCAGGTAGATCACCAGTTCGGAGATGTTGCGCGCATGGTCGCCAATACGCTCCAGCGAGCGCAGCACCCAGATCACGCTCAGCACCCGCGAGATAGA   <  1:860248/236‑1                                                                                                                                                                                                                                                GCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGACAGCAGTGCCCTGCACTTCTGCCGTCATGCGCTTGAGGCCCATGTGGCGCACGTCGGTGCCGCGCACCAGGTAGATCACCAGTTCGGAGATGTTGCGCGCATGGTCGCCAATACGCTCCAGCGAGCGCAGCACCCAGATCACGCTCAGCACCCGCGAGATAGAG  <  1:78380/236‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             GGGTGCTGAGCGTGATCTGGGTGCTGCGCTCGCTGGAGCGTATTGGCGACCATGCGCGCAACATCTCCGAACTGGTGATCTACCTGGTGCGCGGCACCGACGTGCGCCACATGGGCCTCAAGCGCATGACGGCAGAAGTGCAGGGCACTGCTGTCGCCAACGGCGAAAAGGCTAATGTTCCGGGTGAATCTGACGATAAGTAAGGTTGCTCCCGAGCATCGACGCCCGGCCTTGGCCGGGCGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AE015451/6071009‑6070767 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggccgggcgtCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGACAGCAGTGCCCTGCACTTCTGCCGTCATGCGCTTGAGGCCCATGTGGCGCACGTCGGTGCCGCGCACCAGGTAGATCACCAGTTCGGAGATGTTGCGCGCATGGTCGCCAATACGCTCCAGCGAGCGCAGCACCCAGATCACGCTCAGCACCCGCGAGATAGAG  >  AE015451/6070789‑6071020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.