| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 4,022,376 | T→C | 6.3% | intergenic (‑71/+597) | PP_3547 ← / ← emrB | putative NAD(P)‑binding oxidoreductase with NAD(P)‑binding Rossmann‑fold domain/multidrug efflux transport system ‑ permease subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 4,022,376 | 0 | T | C | 6.3% | 186.3 / 4.3 | 64 | intergenic (‑71/+597) | PP_3547/emrB | putative NAD(P)‑binding oxidoreductase with NAD(P)‑binding Rossmann‑fold domain/multidrug efflux transport system ‑ permease subunit |
| Reads supporting (aligned to +/- strand): ref base T (29/30); new base C (2/2); total (31/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.26e-01 | |||||||||||
CGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAACGGACGGTTCGCTGAGTCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCG > AE015451/4022142‑4022595 | cGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCTGCAGCGCGGTCCTTCCGGGAGCAAGCCCGGAGCGa > 1:1009194/1‑236 (MQ=255) cAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGgagggagg > 1:140009/1‑236 (MQ=255) tAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGa > 1:994535/1‑235 (MQ=255) cgcgCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGa > 2:1160176/1‑233 (MQ=255) aaCATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGa > 2:560122/1‑213 (MQ=255) aaCATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGcc > 2:1153468/1‑229 (MQ=255) aaCATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGgatgat > 1:1306101/1‑236 (MQ=255) aaCATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGgat > 2:1033599/1‑233 (MQ=255) aaCATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCAGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGgatgat > 1:1336338/1‑236 (MQ=255) aaCATCAGCACAGCCAACCCGCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCACGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGgat > 2:918720/1‑233 (MQ=255) aCATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAgg > 1:1189575/1‑202 (MQ=255) aCATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCAGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGgatga < 2:1336335/234‑1 (MQ=255) cAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAgg < 2:1058499/220‑1 (MQ=255) ccTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGtttttt < 1:560124/236‑1 (MQ=255) tttgatctggcttttgatctggctTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACtt > 1:1410651/1‑236 (MQ=255) tgatctggcttttgatctggctttTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATCCATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGcc < 1:1420497/200‑1 (MQ=14) cttttgatctggcttttgatctTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGt < 1:922593/213‑1 (MQ=38) tttgatctggcttttgatctTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGtt < 1:918722/235‑1 (MQ=37) tgatcttgatcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAggg < 1:1148048/235‑1 (MQ=31) cttgatcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGtgt > 1:90214/1‑181 (MQ=14) atcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGag < 2:1025115/142‑1 (MQ=32) ttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACaaaa < 2:844089/206‑1 (MQ=31) cgACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCa > 1:498867/1‑235 (MQ=33) gACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGc > 1:572882/1‑236 (MQ=33) gACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGc > 1:778832/1‑236 (MQ=33) gCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATCCTGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTcg < 1:1068626/171‑1 (MQ=9) ggCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACaaaa < 1:1043815/180‑1 (MQ=17) gACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAACGGACGGTTCGCTGAGTCaaaa > 2:772697/1‑233 (MQ=38) ggCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAACGGACGGTTCg > 2:1008121/1‑218 (MQ=38) gCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGcc < 2:1510638/105‑1 (MQ=11) gCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGcc > 1:1510641/1‑105 (MQ=11) aTGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAACGGACGGTTCGCTGAGTCAAAACAGACGAGTTCg > 2:1104194/1‑234 (MQ=40) tGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCTTCGGCGCGGTCCTCGCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACa < 2:350145/146‑1 (MQ=12) cAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCAGTAAGGGCGAAAAGGTg > 2:1395779/1‑171 (MQ=17) gcgcTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACttttt > 2:1140908/1‑124 (MQ=32) ccccAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAACGGACGGTTCGCTGAGTCAAAACAGACGAGTTCGCAtcttgcttcttgcttcttg > 2:221497/1‑235 (MQ=255) cAGGATGGGGCCGTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAACGGACGGTTCGCTGCGTCAAAACAGACGAGTTCGc < 2:1512151/212‑1 (MQ=255) ggATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTTGCCATCGCAAACGGACGGTTCGCTGAGTCAAAACAGACGAGTTCGCAtcttgcttcttgcttcttgcttc < 2:778830/234‑1 (MQ=255) gATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAACGGACGGTTCGCTGAGTCAAAACAGACGAGTTCGCAtcttgcttcttgcttcttgcttcttg < 1:385255/236‑1 (MQ=255) ccTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGGTACTTTTTGTCGCGTGTGACAAACAgg > 2:557418/1‑120 (MQ=255) ttCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGGAAGGGTGactatgc < 2:201539/151‑7 (MQ=9) cggcggGGTCCTGCCGGGAGCAAGGGCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGACGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAACGGACGGTTCGCTGAGTCAAAACAGACGAGTTCGCATCTTgcttcttgcttcttgcttcttgcttctt < 1:450790/231‑1 (MQ=255) ggCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAACGGACGGTTCGCTGAGTCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGAt < 1:1033602/234‑1 (MQ=255) gcgcGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGt > 2:1422014/1‑80 (MQ=34) gcgcGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGGCAAAAAGTGACTccc > 1:110772/1‑120 (MQ=17) gcgcGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGAcac > 2:264052/1‑118 (MQ=17) gcgcGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAgg > 2:1322594/1‑138 (MQ=32) gcgcGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAACGGACGGTTCGCTGAGTCAAAACAGACGAGTTCGCAtcttgct < 1:1008123/204‑1 (MQ=255) cgGTGCTGGTGGGAGCAAGGCCGGAGTGAGGGAAACCCGGGGCGCAGCGAAGGGGCCGGATGAGGGGAGCGCAGCGTTTTGTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCg < 2:104800/118‑1 (MQ=11) gTCCTACCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTg < 2:1187968/111‑1 (MQ=17) cTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAACGGACGGTTCGCTGAGTCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTc < 1:1104197/235‑1 (MQ=255) cTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAACGGACGGTTCGCTGAGTCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTc < 1:1512561/235‑1 (MQ=255) tGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGaca > 2:1108312/1‑124 (MQ=18) cggggAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAACGGACGGTTCGCTGAGTCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTca < 1:488794/231‑1 (MQ=255) ccGGGAGTAAGGCCGGAGGGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAACGGACGGTTCGCTGAGTCAAAACAGACGAGTTCGCAtcttgcttcttgc < 1:481289/199‑1 (MQ=255) cGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAACGGACGGTTCGCTGAGTCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTcac < 2:498865/233‑1 (MQ=255) cGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAACGGACGGTTCGCTGAGTCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATc < 1:221498/236‑1 (MQ=255) gggAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAACGGACGGTTCGCTGAGTCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATc < 1:772699/235‑1 (MQ=255) ggggCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAACGGACGGTTCGCTGAGTCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCAtt < 1:384442/225‑1 (MQ=255) ggAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAACGGACGGTTCGCTGAGTCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCg < 1:1160179/235‑1 (MQ=255) aaGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGCCAAAAAGTGACTCGCCGTAAGGGCGAAAAggc > 2:424613/1‑119 (MQ=17) aaGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGt > 1:566021/1‑124 (MQ=33) aaGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAACGGACGGTTCGCTGAGTCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTcaca < 1:1439308/227‑1 (MQ=255) cGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGGTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTa > 1:276330/1‑102 (MQ=17) ggAGTGAGGGAACCCCGGGGCGCGGCGCAGGGGCCGGAGGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAACGGACGGTTCGc < 2:32397/148‑1 (MQ=255) | CGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAACGGACGGTTCGCTGAGTCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCG > AE015451/4022142‑4022595 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |