| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 5,300,963 | T→G | 5.3% | D937A (GAT→GCT) | recB ← | Chi activated ATP‑dependent DNA helicase and dsDNA/ssDNA exonuclease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 5,300,963 | 0 | T | G | 5.3% | 257.7 / 4.0 | 71 | D937A (GAT→GCT) | recB | Chi activated ATP‑dependent DNA helicase and dsDNA/ssDNA exonuclease |
| Reads supporting (aligned to +/- strand): ref base T (35/32); new base G (2/2); total (37/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01 | |||||||||||
ATTGCAGCGCTGGCCAACGGTACGCTCTATCAGTTGAGGGTTAGCGCTGACCTCGCTGAAGCCCTCACGGCCTGCCCACTCCAGCAAACCATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACATCTGCTCATCCGCCTGCGGCAGGCCTGGGCAAGTGATGTGCGGGCAACTTGCCGCCAAAGCCTGCAACCAGTCCGTCAGCTGCGCCGAGCCAGCCAAGGCAAGGCCGCCCCC > AE015451/5300728‑5301183 | aTTGCAGCGCTGGCCAACGGTACGCTCTATCAGTTGAGGGTTAGCGCTGACCTCGCTGAAGCCCTCACGGCCTGCCCACTCCAGCAAACCATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGAt > 1:1062255/1‑236 (MQ=255) cAGCGCTGGCCAACGGTACGCTCTATCAGTTGAGGGTTAGCGCTGACCTCGCTGAAGCCCTCACGGCCTGCCCACTCCAGCAAACCATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGc > 1:687754/1‑235 (MQ=255) gCCAACGGTACGCTCTATCAGTTGAGGGTTAGCGCTGACCTCGCTGAAGCCCTCACGGCCTGCCCACTCCAGCAAACCATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGa < 1:1176450/235‑1 (MQ=255) ccAACGGTACGCTCTATCAGTTGAGGGTTAGCGCTGACCTCGCTGAAGCCCTCACGGCCTGCCCACTCCAGCAAACCATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAg < 1:1479867/236‑1 (MQ=255) cAACGGTACGCTCTATCAGTTGAGGGTTAGCGCTGACCTCGCTGAAGCCCTCACGGCCTGCCCACTCCAGCAAACCATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAgg < 1:1326621/236‑1 (MQ=255) aaCGGTACGCTCTATCAGTTGAGGGTTAGCGCTGACCTCGCTGAAGCCCTCACGGCCTGCCCACTCCAGCAAACCATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAg < 2:763104/234‑1 (MQ=255) tctATCAGTTGAGGGTTAGCGCTGACCTCGCTGAAGCCCTCACGGCCTGCCCACTCCAGCAAACCATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGAt > 2:1088340/1‑211 (MQ=255) aGTTGAGGGTTAGCGCTGACCTCGCTGAAGCCCTCACGGCCTGCCCACTCCAGCAAACCATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAgg < 2:1337184/219‑1 (MQ=255) ggTTAGCGCTGACCTCGCTGAAGCCCTCACGGCCTGCCCACTCCAGCAAACCATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAgg > 1:1127782/1‑212 (MQ=255) ggTTAGCGCTGACCTCGCTGAAGCCCTCACGGCCTGCCCACTCCAGCAAACCATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAgg < 2:1127779/212‑1 (MQ=255) ggTTAGCGCTGACCTCGCTGAAGCCCTCACGGCCTGCCCACTCCAGCAAACCATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATcc > 1:710272/1‑231 (MQ=255) ttAGCGCTGACCTCGCTGAAGCCCTCACGGCCTGCCCACTCCAGCAAACCATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCCGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACCCGCAGGGCa > 2:270103/1‑213 (MQ=255) aCCTCGCTGAAGCCCTCACGGCCTGCCCACTCCAGCAAACCATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGc < 1:16748/236‑1 (MQ=255) tCGCTGAAGCCCTCACGGCCTGCCCACTCCAGCAAACCATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGATCAGACCCTCGGCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGcc < 2:635518/234‑1 (MQ=255) tCGCTGAAGCCCGCACGGCCTGCCCACGCCAGCAAACCATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGa < 2:124367/195‑1 (MQ=255) cTGAAGCCCTCACGGCCTGCCCACTCCAGCAAACCATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTa < 1:921329/203‑1 (MQ=255) cGGCCTGCCCACTCCAGCAAACCATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATg > 1:1322156/1‑224 (MQ=255) cGGCCTGCCCACTCCAGCAAACCATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATg < 2:1322153/224‑1 (MQ=255) cGGCCTGCCCACTCCAGCAAACCATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATg > 2:1219087/1‑224 (MQ=255) gCCTGCCCACTCCAGCAAACCATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTg > 2:1170608/1‑207 (MQ=255) cACTCCAGCAAACCATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCgcg > 1:286308/1‑227 (MQ=255) cACTCCAGCAAACCATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTg > 2:900526/1‑229 (MQ=255) cAAACCATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAgg < 1:1430447/166‑1 (MQ=255) aCCATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCg < 2:747404/151‑1 (MQ=255) cATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAgg < 2:65537/171‑1 (MQ=255) aTGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGtt > 2:965642/1‑233 (MQ=255) aTGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCgg < 1:913704/236‑1 (MQ=255) ggaggaAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGACggg > 2:51513/1‑159 (MQ=255) aggaAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATg > 1:30277/1‑197 (MQ=255) gCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCTATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGc > 1:721718/1‑232 (MQ=255) ccGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATg < 2:1054777/234‑1 (MQ=255) ccGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTATGATCGCCGACACGAAg < 1:542948/146‑1 (MQ=255) gggTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATa > 1:903836/1‑236 (MQ=255) ggTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGa > 2:766334/1‑233 (MQ=255) ggTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGAGCGCCGACACg > 2:1345133/1‑139 (MQ=255) cccccGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGc > 2:843838/1‑150 (MQ=255) cccccGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATa > 2:667610/1‑228 (MQ=255) cgcgGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACATCTGCTCATc < 1:691053/236‑1 (MQ=255) gcgGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGtgtg < 2:1275528/213‑1 (MQ=255) cgGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACATCTGCTCATCCg > 1:1420391/1‑236 (MQ=255) gggAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACATCTGCTCATCCGc > 1:818349/1‑236 (MQ=255) tGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCt < 2:1371752/148‑1 (MQ=255) gATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGacac > 2:491937/1‑113 (MQ=255) gATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGt < 2:1247201/163‑1 (MQ=255) gATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACATCTGCTCATCCGCCTGCGGCAGGc > 1:1366557/1‑236 (MQ=255) cgcTGTCGGCCGGGCCCTCGCGCAACGCCTGGGCATCGGGCCCCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACATCTGCt < 1:239244/210‑1 (MQ=255) ccGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGacac < 2:88281/96‑1 (MQ=255) gcAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACATCTGCTCATCCGCCGGCGGCAGGCCTGGGCAAGTGATGTGCGGGCAACTTgcc > 1:258799/1‑236 (MQ=255) cAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACATCTGCTCATcc < 1:1514808/193‑1 (MQ=255) acCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCAc < 2:710270/154‑1 (MQ=255) acCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATCGCGTGGTCTGCGTGCTGGCAGCAGCTCACGt > 2:335928/1‑156 (MQ=255) cTGGGCGTCGGGCACGTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGTTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGc < 2:93423/117‑1 (MQ=255) cTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTTGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACATCTGCTCATCCGCCTGCGGCAGGCCTGGGCAAGTGATGTGCGGGCAACTTGCCGCCaaa < 1:799139/235‑1 (MQ=255) gCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATGGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTACTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACATCTGCTCATCCGc > 2:799137/1‑178 (MQ=255) gCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACATCTGCTCATCCGCCTGCGGCAGGCCTGGGCAAGTGATGTGCGGGCAACTTGCCGCCAAAGCCTGCAACCAg > 1:1483351/1‑236 (MQ=255) gCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGATCAGACCCTCGGCGCCGACACGAAGGGCACTGTAGGAAGCGATc < 2:1506677/89‑1 (MQ=16) gCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGATCAGACCCTCGGCGCCGACACGAAGGGCACTGTAGGAAGCGATc > 1:1506680/1‑89 (MQ=21) cgtcgtCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACATCTGCTCATCCGCCTGCGGCAGGCCTGGGCAAGTGATGTGCGGGCAACTTGCCGCCAAAGCCTGCAACCAGTcc > 2:628349/1‑233 (MQ=255) gtcgtcCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCAGAGGGTCTGATCGCCGACTCGCAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACATCTGCTCATCCGCCTGCGGCAGGCCTGGGCaa > 2:528991/1‑190 (MQ=255) cgtcCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACATCTGCTCATCCGCCTGCGGCAGGCCTGGGCAAGTGATGTGCGGGCAACTTGCCGCCAAAGCCTGCAACCAGTCCGt < 1:1284022/232‑1 (MQ=255) ggTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCGGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACATCTGCTCATCCGCCTGCGGCAGGCCTGGGCAAGttgcccg < 2:1188897/187‑7 (MQ=255) tgtgCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACATCTGCTCATCCGCCTGCGGCAGGCCTGGGCAAGTGATGTGCGGGCAACTTGCCGCCAAAGCCt < 1:1027277/205‑1 (MQ=255) gtgCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACATCTGCTCATCCGCCTGCGGCAGGCCTGGGCAAGTGATGTGCGGGCAACTTGCCGCCAAAGCCTGCAACCAGGCCGTCAGCTGCGCCGagccagcc > 1:398519/1‑236 (MQ=255) ccTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACa < 1:1007625/138‑1 (MQ=255) ccTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACATCTGCTCATCCGCCTGCGGCAGGCCTGGGCAAGTGATGTGCGGGCAACTTGCCGCCAAAGCCTGCAACCAGTCCGTCAGCTGCGCCGAGCCAGCcaag > 1:1507091/1‑236 (MQ=255) cTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACATCTGCTCATCCGCCTGCGGCAGGCCTGGGCAAGTGATGTGCGGGCAACTTGCCGCCAAAGCCTGCAACCAGTCCGTCAGCTGCGCCGAGCCAGCcaagg > 1:748556/1‑236 (MQ=255) cttcgtGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACATCTGCTCATCCGCCTGCGGCAGGCCTGGGCAAGTgc > 2:1168697/6‑169 (MQ=255) gCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACATCTGCTCATCCGCCTGCGGCAGGCCTGCGCAAGTGATGTGCGGGCAACTTgcc > 1:1216305/1‑184 (MQ=255) ggCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACATCTGCTCATCCGCCTGCGGCAGGCCTGGGCAAGTGATGTGCGGGCAACTTGCCGCCAAAGCCTGCAACCAg > 2:858578/1‑196 (MQ=255) ggCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACATCTGCTCATCCGCCTGCGGCAGGCCTGGGCAAGTGATGTGCGGGCAACTTGCCGCCAAAGCCTGCAACCAg < 1:858580/196‑1 (MQ=255) cgcCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACATCTGCTCATCCGCCTGCGGCAGGCCTGGGCAAGTGATGTGCGGGCAACTTGCCGCCAAAGCCTGCAACCAGTCCGTCAGCTGCGCCGAGCCAGCCAAGGCAAGGCCGccccc > 1:1324550/1‑235 (MQ=255) ccGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACATCTGCTCATCCGCCTGCGGCAGGCCTGGGCAAGTGATGTGCGGGCAACTTGCCGCCAAAGCCTGCAACCAGTCCGTCAGCTGCGcc < 2:1386193/207‑1 (MQ=255) gAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCGCGGCAGCCGCATGGCGTGGTCGGCGTGCTGGCAGCAGCCCACGTTCGGCGTGTGGCATGCGATACATCTGCTCATCCGCCTGCg > 1:1130089/1‑137 (MQ=255) | ATTGCAGCGCTGGCCAACGGTACGCTCTATCAGTTGAGGGTTAGCGCTGACCTCGCTGAAGCCCTCACGGCCTGCCCACTCCAGCAAACCATGGAGGAAGGTGCCGGGGTTAGGCCCCCGCGGGAAGCGATGGATATCGCCGCTGTCGGCCGGGACCTCGCGCAACACCTGGGCATCGGGCACCTCGTCGTCCAGCAGTTGCTGTGCCTGCGAGCTGTCGGCGCCGAGGGTCTGATCGCCGACACGAAGGGCACTGTAGGAAGCGATCCACCAGTGCTCGGCAGCCGCATGGCGTGGTCTGCGTGCTGGCAGCAGCTCACGTTCGGCGTGTGGCATGCGATACATCTGCTCATCCGCCTGCGGCAGGCCTGGGCAAGTGATGTGCGGGCAACTTGCCGCCAAAGCCTGCAACCAGTCCGTCAGCTGCGCCGAGCCAGCCAAGGCAAGGCCGCCCCC > AE015451/5300728‑5301183 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |