Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	196,390	T→C	16.5%	D632D (GAT→GAC)	PP_0168 →	putative surface adhesion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	196,390	0	T	C	16.5%	92.1 / 9.5	41	D632D (GAT→GAC)	PP_0168	putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base T (20/14);  new base C (5/2);  total (25/16)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC  >  AE015451/196195‑196620                                                                                                                                                                                                    |                                                                                                                                                                                                                                       gCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGCCaacaacga                                                                                                                                                                                                        >  1:299497/1‑226 (MQ=21)           aCCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAgtg                                                                                                                                                                                       <  1:694990/235‑1 (MQ=37)                      cGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGAttt                                                                                                                                                                                                                                      <  2:1367617/177‑1 (MQ=21)                      cGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGAttt                                                                                                                                                                                                                                      >  1:1367620/1‑177 (MQ=25)                        aCTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAgcgg                                                                                                                                                                                      >  1:224351/1‑220 (MQ=14)                               cGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCAACAACAACGGTTCGACCGTCAACGTCACCATCGAAAACGCCa                                                                                                                                                                     >  2:551315/1‑233 (MQ=2)                                        caCCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAAtt                                                                                                                                                         <  1:1524232/235‑1 (MQ=2)                                                                  cGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCa                                                                                                                                                                                          <  2:1234010/177‑1 (MQ=25)                                                                  cGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCa                                                                                                                                                                                          >  1:1234013/1‑177 (MQ=25)                                                                    gTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACg                                                                                                                                                                                                                  >  1:248930/1‑151 (MQ=255)                                                                                                     aCCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACaacaac                                                                                                                                                                                                       >  2:318534/1‑129 (MQ=255)                                                                                                           aGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCcaccac                                                                                      <  1:391957/236‑1 (MQ=37)                                                                                                            gCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTacaa                                                                                                                                                                                                           >  1:999091/1‑118 (MQ=32)                                                                                                            gCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGt                                                                                                                                                             >  2:250898/1‑164 (MQ=38)                                                                                                              aaTCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTCCAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACtggcggc                                                                                                                                                             >  1:287038/1‑161 (MQ=18)                                                                                                               aTCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGcca                                                                                                                                                                                                                        <  2:127899/102‑3 (MQ=17)                                                                                                                  cTGCCCAGACGCCGGTAACAGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCAGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGAcc                                                                                                                   <  1:1173714/200‑1 (MQ=255)                                                                                                                   tGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACcgcg                                                                                  >  1:1201832/1‑232 (MQ=14)                                                                                                                             ccGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAg                                                                                                                                                    >  1:431652/1‑156 (MQ=38)                                                                                                                                      gTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGCACAGCGTCGACTTCCAGACCCCGGCGCATGACGTCTACAACAACgg                                                                                                                                                                                                     >  1:844435/1‑98 (MQ=11)                                                                                                                                           ccTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAActt                                                                                                                                                        >  2:1216109/1‑138 (MQ=255)                                                                                                                                               tCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTacaa                                                                                                                                                                                                           <  2:1087020/83‑1 (MQ=35)                                                                                                                                                ccAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGa                                                                                                                                                                                                                        >  1:149977/1‑69 (MQ=35)                                                                                                                                                ccAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGa                                                                                                                                                                                                                        <  2:149976/69‑1 (MQ=35)                                                                                                                                                ccAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGTGTCACCATCGAGAACGCCACcggcggc                                                                                                                                                             >  2:1350843/1‑127 (MQ=21)                                                                                                                                                 cAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcg                                                <  1:250900/236‑1 (MQ=37)                                                                                                                                                  aaCGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCgg                                                                                                                                                                                                                           >  1:945489/1‑64 (MQ=25)                                                                                                                                                  aaCGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACg                                                                                                                                                                                                      >  1:910125/1‑85 (MQ=35)                                                                                                                                                       ccAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGAcc                                                                                                                               >  1:464282/1‑150 (MQ=38)                                                                                                                                                            ccATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCAcc                                                       >  1:700545/1‑218 (MQ=38)                                                                                                                                                                  ccGGTGAAGCGGGGAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACGGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcg                                                <  1:227307/219‑1 (MQ=12)                                                                                                                                                                   cGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTg                                                                                                                                              >  2:932262/1‑124 (MQ=18)                                                                                                                                                                              gtaaaaCCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAt                                                                                                                                                                                                                      <  2:1518379/39‑1 (MQ=25)                                                                                                                                                                               aaaaaCCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                                              <  1:965837/207‑1 (MQ=38)                                                                                                                                                                                aaaaCCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAt                                                                                                                                                                                                                      >  1:387247/1‑39 (MQ=40)                                                                                                                                                                                aaaaCCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTac                                                                                                                                                                                                             >  2:545371/1‑48 (MQ=38)                                                                                                                                                                                aaaaCCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcg                                                <  2:600239/205‑1 (MQ=39)                                                                                                                                                                                aaaaCCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcg                                                >  1:600241/1‑205 (MQ=40)                                                                                                                                                                                            aGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATc                                                                                                                                                                               >  1:462545/1‑66 (MQ=35)                                                                                                                                                                                               gTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGcc  <  1:967340/236‑1 (MQ=38)                                                                                                                                                                                                 cGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGcc                                                                                                                                                                      <  2:1459845/70‑1 (MQ=35)                                                                                                                                                                                                 cGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCg                                                                                                    <  2:1433281/136‑1 (MQ=25)                                                                                                                                                                                                 cGATTTCCAGACCCCGGCGAATGACGTATACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCg                                                                                                    >  1:1433284/1‑136 (MQ=25)                                                                                                                                                                                                    |                                                                                                                                                                                                                                       GCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC  >  AE015451/196195‑196620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑