| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 213,106 | T→C | 5.1% | N6204N (AAT→AAC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 213,106 | 0 | T | C | 5.1% | 325.8 / 2.6 | 95 | N6204N (AAT→AAC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base T (52/38); new base C (3/2); total (55/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCA > AE015451/212871‑213332 | gTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAAt < 1:260566/236‑1 (MQ=40)gTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGCCACTGCCCCGGACAAt > 1:305370/1‑236 (MQ=255) cgcgAAGGTGGATGTCACCGACACCACCGACGAAGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGt > 1:304739/1‑234 (MQ=255) gTCACCGACACCACCGACGAAGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCaa < 1:1068621/236‑1 (MQ=255) ccGACACCACCGACGAAGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACg < 1:203860/234‑1 (MQ=38) cgaAGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAAt > 2:1025725/1‑202 (MQ=255) tCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATc < 1:379246/236‑1 (MQ=39) gTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCACCGACCCTGTCGTGATGt > 2:1389119/1‑229 (MQ=255) aCCGCCCCCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAg < 1:190906/235‑1 (MQ=255) aCCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAg < 2:678932/235‑1 (MQ=255) ccGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTc < 1:1211870/217‑1 (MQ=39) ccGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGAt > 2:1361338/1‑220 (MQ=255) ccACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGgcg > 1:545341/1‑234 (MQ=40) aCCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGAt > 1:1075835/1‑215 (MQ=39) cGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGAc > 1:220121/1‑194 (MQ=38) cGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACg > 2:1201941/1‑189 (MQ=40) cGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACg < 1:1201944/189‑1 (MQ=255) gTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCa < 1:741001/235‑1 (MQ=255) gTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACCGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGCTCGCAACCGTTGAAGGCGCGGACGCTCGCac > 2:489884/1‑235 (MQ=255) cGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGACACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACg < 2:5657/182‑1 (MQ=37) gAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTAcc > 2:225590/1‑177 (MQ=38) gAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGATGTCGGCAAGTTc < 2:1028421/234‑1 (MQ=255) gaTTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACg > 1:694442/1‑235 (MQ=40) cacCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAg < 1:934984/235‑1 (MQ=255) tCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGAt > 2:769430/1‑169 (MQ=18) tCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACa > 2:599879/1‑228 (MQ=255) cGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACaa > 2:1160243/1‑226 (MQ=255) cGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGAcc > 2:324390/1‑156 (MQ=14) cGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAg > 1:1207508/1‑186 (MQ=14) gCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCCACCGtt > 2:1165981/1‑181 (MQ=37) gCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGtt > 1:370447/1‑196 (MQ=40) gCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGtt > 2:1092288/1‑196 (MQ=255) gCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCa < 1:1224129/236‑1 (MQ=255) gACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAg < 1:106880/223‑1 (MQ=255) caacaaAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAg < 2:1475283/220‑1 (MQ=255) aacaaAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGTACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGt > 2:1368617/1‑196 (MQ=255) aacaaAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGAc > 1:92415/1‑221 (MQ=255) aacaaAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTc > 1:1025651/1‑236 (MQ=255) aacaaAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTc > 1:1275065/1‑236 (MQ=255) aCGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTg < 1:749669/149‑1 (MQ=39) gtcTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACcgac < 1:565932/234‑1 (MQ=255) gCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCa < 2:1038778/222‑1 (MQ=255) ccGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCTCCGGACAATGGCTACGTCGGTACCAACGACCCTGTCGTGATGTCCATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGc < 1:1436794/235‑1 (MQ=255) cGATTAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACcgac < 2:1286374/230‑1 (MQ=11) cGATTAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACcgac > 1:1286377/1‑230 (MQ=11) acaGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTAGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTCGACAAGACGCCGGTCAGCACGTCGGTGa > 1:820115/1‑213 (MQ=255) caGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTc < 1:1106882/199‑1 (MQ=255) aTTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGCGCAACTGACGCTGGACAAGACGCCGGTCAGCAc > 2:1197067/1‑198 (MQ=255) aTTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAg > 1:419993/1‑236 (MQ=255) aTTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAg > 1:187146/1‑236 (MQ=255) ttGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGCTg > 2:231742/1‑123 (MQ=38) tGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACcgac < 2:1305620/209‑1 (MQ=255) tGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACcgac > 1:1305623/1‑209 (MQ=255) gACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAgg < 2:819862/234‑1 (MQ=255) aCCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGAcac > 1:901866/1‑170 (MQ=255) ccTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCaa > 2:395361/1‑154 (MQ=255) ccTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGAc > 1:1181782/1‑236 (MQ=255) ttCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGAcct > 1:1397823/1‑234 (MQ=255) ttCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACtt > 1:1411885/1‑236 (MQ=255) ttCACGCGGAGCGACGGCAAACCCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCa < 2:124573/151‑1 (MQ=255) acccTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAATGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTc > 2:614625/4‑90 (MQ=255) acccTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTc < 1:614627/87‑1 (MQ=255) gCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACg > 1:1290278/1‑175 (MQ=255) gCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTAc > 2:705095/1‑210 (MQ=255) gCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCa > 1:399695/1‑236 (MQ=255) tGAGCGACGGCAAAACCGTCATCACCGTGCCGGCGAATGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTc < 2:1051212/179‑1 (MQ=255) gCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGCTGTCGATCGCAAcc > 2:696286/1‑119 (MQ=255) ggCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACcgac < 1:145594/189‑1 (MQ=255) aaCCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCa < 2:1028111/233‑1 (MQ=255) ccGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACACGACGCCGGTCAGCACGGCGGTGGCCGACGAGCCGGGGACCCCAGGc > 2:178779/1‑200 (MQ=255) gTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGCCGCCGGTCAGCACGTCGGTGAc > 1:767229/1‑176 (MQ=255) aCCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAg < 2:454412/147‑1 (MQ=255) aCCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAg > 1:454414/1‑147 (MQ=255) cGTGCCGGCCAACGGCACCGCGGGCCCTGCCCCCGTCACGGCCCCGGACAATGTCTACGTCGGTACCAACGCCCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCAc < 2:352656/216‑1 (MQ=255) ggCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACcgac > 2:1129405/1‑166 (MQ=255) ggCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACcgac < 1:1129408/166‑1 (MQ=255) gCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCTGTAc > 2:289210/1‑59 (MQ=255) gCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGGACCCCAGGCacc > 2:93596/1‑184 (MQ=255) aaCGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGa > 1:125752/1‑79 (MQ=255) aaCGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGa < 2:125751/79‑1 (MQ=21) cACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTc > 2:229046/1‑139 (MQ=255) cACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTc < 1:229047/139‑1 (MQ=255) cGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTc < 1:984823/235‑1 (MQ=255) gTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTc < 1:591360/234‑1 (MQ=255) cgggTACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGt < 2:60189/57‑1 (MQ=255) gggCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACa > 2:77285/1‑122 (MQ=255) gCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTg > 2:1353659/1‑59 (MQ=255) tGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACg < 1:79401/196‑1 (MQ=255) ggCCACCGGCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACGTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTc < 2:32790/225‑1 (MQ=255) cGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGcc > 1:715146/1‑211 (MQ=255) gTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCAcc < 2:1181779/234‑1 (MQ=255) tCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGAcca > 2:212812/1‑200 (MQ=255) acggCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCt < 2:143879/196‑1 (MQ=255) cTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACg < 2:865052/156‑1 (MQ=255) gCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGc > 2:279441/1‑159 (MQ=255) cccGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCa > 1:84717/1‑236 (MQ=255) gACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGcac > 1:21813/1‑228 (MQ=255) aCAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCa > 2:289558/1‑140 (MQ=255) | GTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCA > AE015451/212871‑213332 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |