| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 213,757 | T→C | 8.3% | A6421A (GCT→GCC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 213,757 | 0 | T | C | 8.3% | 294.9 / 7.3 | 93 | A6421A (GCT→GCC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base T (48/37); new base C (3/5); total (51/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTAGTGGCCAAGTTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTG > AE015451/213531‑213983 | gcAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTAGTGGCCAAGTTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACaa > 1:1032518/1‑235 (MQ=255) gCTTCGGTTCAAGTGACCGACACCACCGACGAAGTAGTGGCCAAGTTGACCGCGCCCCCGTCGGTGACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTa < 1:273100/236‑1 (MQ=255) cGGTTCAAGTGACCGACACCACCGACGAAGTAGTGGCCAAGTTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGt > 1:1033897/1‑235 (MQ=255) cGGTTCAAGTGACCGACACCACCGACGAAGTAGTGGCCAAGTTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTc > 1:1366260/1‑236 (MQ=255) cgtcaCCGACACCACCGACGAAGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTGACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGt < 2:762043/224‑1 (MQ=17) aCCGACACCACCGACGAAGTAGTGGCCAAGTTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTAGGTACTGCCACCGTGACTGCTCCGGACAACg > 2:1065590/1‑217 (MQ=255) ccaccacCGACGAAGTAGTGGCCAAGTTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCACCAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCg < 1:289212/205‑1 (MQ=255) acacCACCGACGAAGTAGTGGCCAAGTTGACCGCGACCCCGGCGGTGACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCt < 1:289560/203‑1 (MQ=38) caccGACGAAGTAGTGGCCAAGTTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCCCCGGACaa > 2:1400587/1‑207 (MQ=37) ccGACGAAGTAGTGGCCAAGTTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGt > 1:551406/1‑208 (MQ=255) agtagtGGCCAAGTTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGa < 1:686368/236‑1 (MQ=40) gtagtGGCCATGTTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGC‑TTGACCTTCTCGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGa < 2:806402/234‑1 (MQ=255) cgACCCCGTCGGTGACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGaa > 2:1422154/1‑230 (MQ=40) caCCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCg > 1:11100/2‑235 (MQ=37) ccccGTCGGTGACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTAc < 2:523097/220‑1 (MQ=255) ccccGTCGGTGACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCg < 2:552616/233‑1 (MQ=255) cGTCGGTGACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGTCACCGTGACTGCTcc < 2:1419982/166‑1 (MQ=39) cGTCGGTGACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTcc > 1:1419985/1‑166 (MQ=39) ggTGACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGAGGTGCGGACGTCggta < 1:317378/236‑3 (MQ=37) gAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCTGACAACGTCTACGACGGTATCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTc < 2:802384/234‑1 (MQ=255) ttACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACATCCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGCCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACg < 1:551128/156‑1 (MQ=255) acCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAg < 2:895309/234‑1 (MQ=255) cGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTCCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGt > 1:518177/1‑181 (MQ=255) gCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCATGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGGACCAACGACCCTGTCGt > 2:341222/1‑162 (MQ=255) gCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCg < 2:322514/127‑1 (MQ=38) gCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCaa < 2:889149/150‑1 (MQ=40) gCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGCCCCTGTCGt > 2:397799/1‑162 (MQ=38) gCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGt > 2:1345661/1‑234 (MQ=255) gCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCa > 1:227210/1‑236 (MQ=255) gCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCGGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTc < 2:76034/168‑1 (MQ=38) ggCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCg < 2:162644/174‑1 (MQ=255) gCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGCCAACGTCTACGTCga > 2:119949/1‑126 (MQ=37) gCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACAGTGACTGCTCCGGACAACGTCTACGTCGGTAc > 2:1518988/1‑130 (MQ=255) gCCTGCCGATCAACAACCACAGGGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCg < 2:390259/173‑1 (MQ=255) cTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGAcccc > 1:975067/1‑212 (MQ=255) gCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGc > 1:438104/1‑236 (MQ=255) ccGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTg > 1:48103/1‑166 (MQ=255) ccGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGtt > 1:886697/1‑182 (MQ=40) cGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGc < 2:1207505/234‑1 (MQ=255) gCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAg > 2:1112144/1‑162 (MQ=255) cATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACCCGACAAGACCCCGGTCAGCACTTCGGTCGCcga > 2:290408/1‑211 (MQ=255) ttGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGGCAGc > 2:298140/1‑195 (MQ=255) tGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTATCAACGACCCTATCGTGATGTCGATCGCTACCGTTGAAGGTGCGGTCGTTGGCAAGTTCGAGCGACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGc < 1:1436582/236‑1 (MQ=255) aCCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCg > 1:1452410/1‑235 (MQ=255) aCCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACCCGCCAAGACCCCGGTCAGCACTTCGGTCACCGACGCGcc > 2:292952/1‑212 (MQ=255) aCCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGTGCGGACAGCGGCAAGTCCGAGCAACTGACACTCGACAAGACCCCGGt > 2:835722/1‑188 (MQ=255) ccTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCAGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACCTCGGTCACCGACGAGCCGGGTACCCCAGCCAACGAAGGCAAc > 1:1101117/1‑236 (MQ=255) ttCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCa > 2:330124/1‑151 (MQ=255) ttCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCa < 1:330126/151‑1 (MQ=255) ttCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGc > 1:652819/1‑189 (MQ=255) aCGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTg > 1:153493/1‑106 (MQ=40) aCGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGaa > 2:547229/1‑225 (MQ=2) cGCTTAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTc < 1:869287/236‑1 (MQ=255) gCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCg < 2:319184/73‑1 (MQ=255) gCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACa > 2:829307/1‑170 (MQ=255) gCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAgg > 1:797393/1‑216 (MQ=255) gCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAgg < 2:797391/216‑1 (MQ=255) gCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCACCGACgg > 2:448481/1‑225 (MQ=255) gCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCCGTACCAACGACCCTGGCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCCAGTTc > 2:365941/1‑151 (MQ=255) gCGACGGCAAAACCGTCATCACCGTGCCGGCCAACTGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGCGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTc > 2:202137/1‑146 (MQ=255) gCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTGCCAACGACCCTGTCGTGATGTCGATCGCAAcc > 1:1151556/1‑119 (MQ=255) gCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAgg > 2:604097/1‑211 (MQ=255) gACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGt > 2:1340663/1‑227 (MQ=255) gACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTc > 2:1068816/1‑234 (MQ=255) aCGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCAc < 1:1070510/235‑1 (MQ=255) gCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCAcc < 1:667629/185‑1 (MQ=255) aaaaCCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGt > 2:855042/1‑128 (MQ=255) aaaaCCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCa < 1:756615/235‑1 (MQ=255) aCCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCAcc < 2:1276153/180‑1 (MQ=255) ccGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCg < 1:1350984/56‑1 (MQ=255) ccGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACtt > 1:250380/1‑171 (MQ=255) catcaCCGTGCCGTCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCg < 2:257333/206‑1 (MQ=255) cGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACGCTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCt < 1:769432/236‑1 (MQ=255) cGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTg > 1:1401857/1‑236 (MQ=255) ggCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACGTCGGTCACCGCCGAGCCGGGTACCCCAGGCAACg > 2:596432/1‑188 (MQ=255) ggCCAACGGCACCGTGGGCACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTg > 1:176855/1‑95 (MQ=255) gCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGc < 1:1340666/236‑1 (MQ=255) cGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTc < 1:1396760/235‑1 (MQ=255) cGTGGGCACTGCCACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACGCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTc < 1:1165984/235‑1 (MQ=255) cGTGGGCACTGCCACCGTCACTGCCCCCGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTc < 1:1504077/235‑1 (MQ=255) gTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCggg > 2:118117/1‑157 (MQ=255) gTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAAc < 2:1032515/234‑1 (MQ=255) gTACTGCCACCGTCACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTg < 1:1353662/59‑1 (MQ=255) aCTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAAcc < 2:1277385/233‑1 (MQ=255) cTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGcc > 1:1159737/1‑151 (MQ=255) cTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGcc < 2:1159734/151‑1 (MQ=255) cTGCCACCGTGACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGaca > 2:674201/1‑110 (MQ=255) ccACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCt < 1:1141166/234‑1 (MQ=255) cACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACTAAGGAGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCt < 2:802846/233‑1 (MQ=255) aCCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGGCACCATCACCGCCGACCAGACCTCgg > 1:937711/1‑208 (MQ=255) ccGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGATGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACt < 1:517497/236‑1 (MQ=255) cGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACtg > 1:490265/1‑236 (MQ=255) gACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCAccgcc < 2:227209/190‑1 (MQ=255) gCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAGCTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGa > 2:90822/1‑161 (MQ=255) | GCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTAGTGGCCAAGTTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTG > AE015451/213531‑213983 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |