Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AE015451 1,435,822 A→T 11.6% C48S (TGC→AGC)  PP_1256 ← putative alpha‑ketoglutarate semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154511,435,8220AT11.6% 216.1 / 19.2 69C48S (TGC→AGC) PP_1256putative alpha‑ketoglutarate semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (33/28);  new base T (4/4);  total (37/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.89e-01

GCACGTCCAGCCATTCGCCGGCCCGCACTGTACGGGCAAAGGTGCGTAGCTGGGTGCAGGTACGGCCACGCTCGCCCTGAATGCGCGCCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGCCTCCTTGGAAATCTAATGCAGATGCATGGGGGGGATTACGACCCCTGTAGGAGCGGGTTCACCCGCGAACACCGGCGAAGCCGGTGC  >  AE015451/1435588‑1436055
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                         
gCACGTCCAGCCATTCGCCGGCCCGCACTGTACGGGCAAAGGTGCGTAGCTGGGTGCAGGTACGGCCACGCTCGCCCTGAATGCGCGCCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAg                                                                                                                                                                                                                                          <  1:1280460/236‑1 (MQ=255)
    gTCCAGCCATTCGCCGGCCCGCACTGTACGGGCAAAGGTGCGTAGCTGGGTGCAGGTACGGCCACGCTCGCCCTGAATGCGCGCCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCTGCGCGCTgg                                                                                                                                                                                                                                         >  2:1365764/1‑233 (MQ=255)
        aGCCATTCGCCGGCCCGCACTGTACGGGCAAAGGTGCGTAGCTGGGTGCAGGTACGGCCACGCTCGCCCTGAATGCGCGCCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTgg                                                                                                                                                                                                                                    <  2:520017/234‑1 (MQ=255)
         gCCATTCGCCGGCCCGCACTGTACGGGCAAAGGTGCGTAGCTGGGTGCAGGTACGGCCACGCTCGCCCTGAATGCGCGCCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGc                                                                                                                                                                                                                                  <  1:988820/235‑1 (MQ=255)
            aTTCGCCGGCCCGCACTGTACGGGCAAAGGTGCGTAGCTGGGTGCAGGTACGGCCACGCTCGCCCTGAATGCGCGCCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTgg                                                                                                                                                                                                                                    <  1:78124/230‑1 (MQ=255)
                 ccGGCCCGCACTGTACGGGCAAAGGTGCGTAGCTGGGTGCAGGTACGGCCACGCTCGCCCTGAATGCGCGCCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTc                                                                                                                                                                                                                         >  1:276879/1‑236 (MQ=255)
                        gCACTGTACGGGCAAAGGTGCGTAGCTGGGTGCAGGTACGGCCACGCTCGCCCTGAATGCGCGCCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTgg                                                                                                                                                                                                                                    <  1:1341867/218‑1 (MQ=255)
                           cTGTACGGGCAAAGGTGCGTAGCTGGGTGCAGGTACGGCCACGCTCGCCCTGAATGCGCGCCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCTGCGCGCTGGCCTGGGCCACGTGTTCGCCTGTgc                                                                                                                                                                                                                 <  1:1365767/234‑1 (MQ=255)
                              tACGGGCAAAGGTGCGTAGCTGGGTGCAGGTACGGCCACGCTCGCCCTGAATGCGCGCCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCa                                                                                                                                                                                                                                           <  1:1366853/205‑1 (MQ=255)
                                 gggCAAAGGTGCGTAGCTGGGTGCAGGTACGGCCACGCTCGCCCTGAATGCGCGCCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGa                                                                                                                                                                                                           >  2:1309868/1‑234 (MQ=255)
                                 gggCAAAGGTGCGTAGCTGGGTGCAGGTACGGCCACGCTCGCCCTGAATGCGCGCCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAg                                                                                                                                                                                                          >  1:527317/1‑235 (MQ=255)
                                           gCGTAGCTGGGTGCAGGTACGGCCACGCTCGCCCTGAATGCGCGCCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCt                                                                                                                                                                                               >  1:1233099/1‑236 (MQ=255)
                                            cGTAGCTGGGTGCAGGTACGGCCACGCTCGCCCTGAATGCGCGCCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTc                                                                                                                                                                                              <  1:1032800/236‑1 (MQ=255)
                                                     gTGCAGGTACGGCCACGCTCGCCCTGAATGCGCGCCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTg                                                                                                                                                                                     <  1:1072222/236‑1 (MQ=255)
                                                                   aCGCTCGCCCTGAATGCGCGCCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCTGCGCGCTGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTggg                                                                                                                                                                        >  1:605557/1‑235 (MQ=255)
                                                                   aCGCTCGCCCTGAATGCGCGCCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTggg                                                                                                                                                                        >  1:1109241/1‑235 (MQ=255)
                                                                     gCTCGCCCTGAATGCGCGCCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTc                                                                                                                                                                      <  1:1186759/235‑1 (MQ=255)
                                                                         gCCCTGAATGCGCGCCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCTGCGCGCTGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCggtg                                                                                                                                                                              <  2:605555/223‑1 (MQ=255)
                                                                                  gcgcgcCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGc                                                                                                                                                                                                <  2:574418/196‑1 (MQ=255)
                                                                                  gcgcgcCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGc                                                                                                                                                                                                >  1:574420/1‑196 (MQ=255)
                                                                                      gcCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGCTg                                                                                                                                                         >  1:164334/1‑231 (MQ=255)
                                                                                      gcCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTc                                                                                                                                                    >  1:1248100/1‑236 (MQ=255)
                                                                                         gtGGGCAGGCCGGTTTCGATCACGGCGCGGTCGAGCAGGGCATCGCCGAGGGCCTCGAGCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTgc                                                                                                                                                                                                                 <  2:172422/171‑1 (MQ=255)
                                                                                                    ggTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGt                                                                                                                                        >  2:497221/1‑234 (MQ=255)
                                                                                                     gTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTAc                                                                                                                                     >  1:761549/1‑236 (MQ=255)
                                                                                                     gTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGAAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCTGCGCGCTGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTAc                                                                                                                                     >  1:1231598/1‑236 (MQ=255)
                                                                                                         cGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGc                                                                                                                                                       >  2:1383473/1‑214 (MQ=255)
                                                                                                          gATCACGGCGCGGTCGATCAGGGCATCGCCGA‑GGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCtgtg                                                                                                                                                                                                                  <  1:1481078/153‑1 (MQ=255)
                                                                                                             cACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGAAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCTGCGCGCTGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCctc                                                                                                                              <  2:1231595/235‑1 (MQ=255)
                                                                                                              aCGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGGATGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTg                                                                                                                            >  1:932422/1‑236 (MQ=255)
                                                                                                                ggCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGt                                                                                                                                        <  1:13810/222‑1 (MQ=255)
                                                                                                                         gATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATcagca                                                                                                                 <  1:140671/236‑1 (MQ=255)
                                                                                                                         cttgaagccATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATcagca                                                                                                                 >  1:402910/9‑236 (MQ=255)
                                                                                                                          aTCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATcagca                                                                                                                 <  1:1309871/235‑1 (MQ=255)
                                                                                                                                   tCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGAt                                                                                                                      <  1:1178210/221‑1 (MQ=255)
                                                                                                                                    cGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGGTACCGGCCTCTGGCCGCTCAGCAGGTGGCCtgt                                                                                                       >  1:252986/1‑235 (MQ=255)
                                                                                                                                      ccGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGAcgg                                                                                                                                                                                 >  1:344502/1‑157 (MQ=255)
                                                                                                                                               tCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGt                                                                                                                                        <  2:223880/191‑1 (MQ=255)
                                                                                                                                                cGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTcgc                                                                                                                                                  <  1:502077/180‑1 (MQ=255)
                                                                                                                                                 gATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGc                                                                                         >  1:1456013/1‑236 (MQ=255)
                                                                                                                                                        gtgGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGcc                                                                                                                         >  2:369248/1‑197 (MQ=255)
                                                                                                                                                          ggCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGt                                                                                            <  1:1358285/224‑1 (MQ=255)
                                                                                                                                                          ggCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGt                                                                                            >  2:1358282/1‑224 (MQ=255)
                                                                                                                                                                    tCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGcc                                                                                                                         >  1:171235/1‑185 (MQ=255)
                                                                                                                                                                    tCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGCCTCCtt                                                                                   >  1:198074/1‑223 (MQ=255)
                                                                                                                                                                    tCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGCCTCCTTGGAAATCTAAt                                                                        >  2:1218433/1‑234 (MQ=255)
                                                                                                                                                                    tCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGCCTCCTTGGAAATCTAATg                                                                       >  1:1284075/1‑235 (MQ=255)
                                                                                                                                                                    tCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGCCTCCTTGGAAATCTAATGc                                                                      >  1:994815/1‑236 (MQ=255)
                                                                                                                                                                    tCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGGCGCGGCTGCCGGGTACCGGCCGCTGGcc                                                                                                                         >  1:165902/1‑185 (MQ=255)
                                                                                                                                                                    tCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGGCGCGGCGGCCGGTTCCCGGCCTCTGGCCGAt                                                                                                                      >  1:3267/1‑188 (MQ=255)
                                                                                                                                                                             tctGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGCCTCCTTGGAAATCTAATGCAGATGCATg                                                             <  1:1218436/236‑1 (MQ=255)
                                                                                                                                                                                 cTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCTGCGCGCTGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCg                                                                                                                        >  1:767985/1‑173 (MQ=255)
                                                                                                                                                                                 cTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCTGCGCGCTGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCg                                                                                                                        <  2:767983/173‑1 (MQ=255)
                                                                                                                                                                                  tCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTg                                                                                                            >  1:630069/1‑184 (MQ=255)
                                                                                                                                                                                      tgttCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGCCTCCTTGGAAATCTAATGCAGATGCATGGGGGGGAtt                                                    <  1:526509/236‑1 (MQ=255)
                                                                                                                                                                                            agtgATGTGTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGATGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGCCTCCTTGGAAATCTAATGCAGATGCATggg                                                           <  1:526317/220‑1 (MQ=255)
                                                                                                                                                                                                aTGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGCCTCCTTGGAAATCTAATGCAGATGCATGGGGGGGATTAc                                                  <  2:949797/228‑1 (MQ=255)
                                                                                                                                                                                                       gcgGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCtgtg                                                                                                      <  2:248939/169‑1 (MQ=255)
                                                                                                                                                                                                        cgGTAGGCATCGAAGGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGCCTCCTTGGAAATCTAATGCAGATGCATGGGGGGGAtt                                                    <  2:1009789/218‑1 (MQ=255)
                                                                                                                                                                                                           tAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGCCTCCTTGGAAATCTAATGCAGATGCATGGGGGGGATTACGACCCCTGTAGGAGCGGGt                               <  1:369250/236‑1 (MQ=255)
                                                                                                                                                                                                              gCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGCCTCCTTGGAAATCTAATGCAGATGCATGGGGGGGATTACGACCCCTGTAGGAGCGGGtt                              <  1:103316/234‑1 (MQ=255)
                                                                                                                                                                                                                      cgtggccCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGCCTCCTTGGAAATCTAATGCAGATGCATgggg                                                          >  1:647501/5‑198 (MQ=255)
                                                                                                                                                                                                                        ccgccCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGCCTCCTTGGAAATCTAATGCAGATGCATGGGGGGGATTACGa                                                >  2:1342154/1‑206 (MQ=255)
                                                                                                                                                                                                                           cccAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGCCTCCTTGGAAATCTAATGCAGATGCATGGGGGGGATTACGACCCCTGTAGGAGCGGGTTCACCCGCGAACACCg               >  1:926360/1‑236 (MQ=255)
                                                                                                                                                                                                                            ccAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGGTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCgcg                                                                                                                      >  2:373944/1‑130 (MQ=255)
                                                                                                                                                                                                                              aGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTTGCCTCCTTGGAAATCTAATGCAGAt                                                                  >  1:64509/1‑182 (MQ=255)
                                                                                                                                                                                                                                gCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGCCTCCTTGGAAATCTAATGCAGATGCATGGGGGGGATTACGACCCCTGTAGGAGCGGGTTCACCCGCGAACACCGGCGa           <  1:1383476/235‑1 (MQ=255)
                                                                                                                                                                                                                                  cAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGCCTCCTTGGAAATCTAATGCAGATGCATGGGGGGGATTACGACCCCTGTAGGAGCGGGTTCACCCGCGAACACCGGCGa           <  2:527315/233‑1 (MQ=255)
                                                                                                                                                                                                                                      gcgcAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGCCTCCTTGGAACTCTAATGCAGATGCATGGGGGGGATTACGACCCCTGTAGGAGCGGGTTCACCCGCGAACACCGGCGAAGCCgg     >  1:838958/1‑235 (MQ=255)
                                                                                                                                                                                                                                        gcAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGCCTCCTTGGAAATCTAATGCAGATGCATGGGGGGGATTACGACCCCTGTAGGAGCGGGTTCACCCGCGAACACCGGCGAAGCCGGTGc  >  1:1431288/1‑236 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                         
GCACGTCCAGCCATTCGCCGGCCCGCACTGTACGGGCAAAGGTGCGTAGCTGGGTGCAGGTACGGCCACGCTCGCCCTGAATGCGCGCCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGCCTCCTTGGAAATCTAATGCAGATGCATGGGGGGGATTACGACCCCTGTAGGAGCGGGTTCACCCGCGAACACCGGCGAAGCCGGTGC  >  AE015451/1435588‑1436055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑