Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AE015451 4,586,033:1 +G 100% intergenic (+116/+99) PP_4061 → / ← PP_4063 conserved protein of unknown function/putative Long‑chain‑fatty‑acid‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154514,586,0331.G100.0% 142.9 / NA 42intergenic (+116/+99)PP_4061/PP_4063conserved protein of unknown function/putative Long‑chain‑fatty‑acid‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (19/23);  total (19/23)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCAGGG‑A‑C‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACC  >  AE015451/4585809‑4586241
                                                                                                                                                                                                                                 |                                                                                                                                                                                                                   
cTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                                                                                                                                                           <  1:886408/236‑1 (MQ=255)
                    aagaagACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                                                                                                                                                           <  2:1141116/216‑1 (MQ=255)
                                        ccGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATgcg                                                                                                                                                                        <  2:1151825/229‑1 (MQ=255)
                                        ccGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGC‑gggt                                                                                                                                                                                                                 >  2:803092/1‑187 (MQ=255)
                                             cAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                                                                                                                                                           <  1:1050656/191‑1 (MQ=255)
                                              aaGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAAcc                                                                                                                                                                                                             <  1:730240/188‑1 (MQ=255)
                                                      cgggccgccGACAGGGGGCAACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                                                                                                                                                           <  1:283914/179‑1 (MQ=255)
                                                                     gggCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCt                                                                                                                                                                                >  1:613334/1‑192 (MQ=255)
                                                                      ggCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAag                                                                                                                                    <  1:1007033/235‑1 (MQ=255)
                                                                          aCCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGCC‑C‑GTGCCGGCCTTGaa                                                                                                                                                                            >  1:1255544/1‑191 (MQ=255)
                                                                            ccgccgAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                                                                                                                                                           <  1:353596/160‑1 (MQ=255)
                                                                                                         ttAAGGGTAGACCACGGCACCGGCGTGAGCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGcccc                                                                                                                                                              <  1:667532/174‑1 (MQ=255)
                                                                                                              ggTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAggcc                                                                                                                               >  2:736049/1‑200 (MQ=255)
                                                                                                              ggTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAggcc                                                                                                                               <  1:736052/200‑1 (MQ=255)
                                                                                                               gTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGcccc                                                                                                                                                              >  1:3784/1‑168 (MQ=39)
                                                                                                                   aCCACGCCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAggcc                                                                                                                               <  2:769395/195‑1 (MQ=39)
                                                                                                                    ccACGGCACCGGCGTGACCAGCTGCTCGGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGGGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAag                                                                                                                                    <  2:659639/189‑1 (MQ=38)
                                                                                                                    ccACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACgcgc                                                                                                                                                                                                          >  2:532333/1‑121 (MQ=255)
                                                                                                                    ccACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                                                                                                                                                         >  1:878807/1‑168 (MQ=39)
                                                                                                                     cACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGC‑gggta                                                                                                                                                                                                                >  1:1212836/1‑110 (MQ=255)
                                                                                                                       cGGCGCTGGCGTGAACAGCTGATCTGCGCTGCTCCATGGAAAGGTGGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATgcggcg                                                                                                                                                                     <  2:76051/153‑1 (MQ=38)
                                                                                                                       cGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATgcgg                                                                                                                                                                       <  2:102930/151‑1 (MQ=39)
                                                                                                                       cGGCACCGGCGTGAACAGCTGAGCTGCGCTGCTCCATGGAAAGGGTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGAATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCt                                                                                                                                                                                                      <  2:94073/122‑1 (MQ=38)
                                                                                                                        ggCACCGGCGGGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGC‑gggtaaa                                                                                                                                                                                                              <  2:251519/112‑6 (MQ=255)
                                                                                                                          cACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCca                                                                                >  1:583826/1‑235 (MQ=255)
                                                                                                                           aCCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGGGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCCCCTACCAgg                                                                                                                                                                                                >  1:176833/1‑124 (MQ=38)
                                                                                                                                       aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAAcc                                                                                                                                                                                                             >  2:786409/1‑99 (MQ=39)
                                                                                                                                            aTCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATgcggcg                                                                                                                                                                     >  2:753500/1‑132 (MQ=38)
                                                                                                                                                         cATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGC‑gggg                                                                                                                                                                                                                 >  2:151737/1‑74 (MQ=255)
                                                                                                                                                            tGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                                                                                                                                                           >  2:120606/1‑80 (MQ=38)
                                                                                                                                                            tGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                                                                                                                                                           <  1:120606/80‑1 (MQ=38)
                                                                                                                                                                      aGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTc                                                                                                                                                                                                        <  2:464117/73‑1 (MQ=25)
                                                                                                                                                                      aGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTc                                                                                                                                                                                                        >  1:464120/1‑73 (MQ=25)
                                                                                                                                                                        gATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCCTGCgg                                                            >  1:295748/1‑209 (MQ=39)
                                                                                                                                                                              cGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCcac                                                                               <  2:1224675/184‑1 (MQ=255)
                                                                                                                                                                                ggTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAg                                                                                                                                                                                                 >  1:253458/1‑70 (MQ=21)
                                                                                                                                                                                        gCAAGGTGAGGATCTTGCATTGACCGGGCCGGCCTCTTCGCGGGTAAACCCGCTGCTACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGttt                                                                                                                                              <  2:207943/111‑1 (MQ=25)
                                                                                                                                                                                           aGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACa               <  1:747208/235‑1 (MQ=255)
                                                                                                                                                                                              tGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTg                                                                                                                                                                              >  2:357414/1‑73 (MQ=18)
                                                                                                                                                                                                          aTTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                                                                                                                                                         <  2:1047957/82‑1 (MQ=18)
                                                                                                                                                                                                          aTTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGc                                                                                           <  2:968808/144‑1 (MQ=37)
                                                                                                                                                                                                          aTTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGC‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCCGGTAGGAGCGGGtt                                                                                                                                               >  1:757027/1‑92 (MQ=21)
                                                                                                                                                                                                           ttGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGCTGCAGCCGCAATCTCCGCCACACTGATCTCACg                                                                   >  1:230563/1‑167 (MQ=38)
                                                                                                                                                                                                              aTCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATAcc  <  2:583823/229‑1 (MQ=255)
                                                                                                                                                                                                                 gggCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GGGCCGGCCTTGAATGCGGCGCTGCCCCTggaa                                                                                                                                                         >  2:138594/1‑73 (MQ=17)
                                                                                                                                                                                                                     cggccTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑A‑CTGTGCCGGCCTTGAATgc                                                                                                                                                                         >  2:1278851/1‑55 (MQ=11)
                                                                                                                                                                                                                                 |                                                                                                                                                                                                                   
CTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCAGGG‑A‑C‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACC  >  AE015451/4585809‑4586241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑