| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 336,125:1 | +T | 6.7% | coding (210/237 nt) | PP_0278 → | conserved protein of unknown function |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 336,125 | 1 | . | T | 6.7% | 353.0 / 18.0 | 89 | coding (210/237 nt) | PP_0278 | conserved protein of unknown function |
| Reads supporting (aligned to +/- strand): ref base . (54/29); new base T (2/3); total (57/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.85e-01 | |||||||||||
TCTCCTTCCGTGAATAAGGATTTGCCATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑T‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGAAAACGGGGGCCGCTTTGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCCGCTTGCGCTAACCACAAG > AE015451/335890‑336347 | tctcCTTCCGTGAATAAGGATTTGCCATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑t < 1:1147080/236‑1 (MQ=255) ccTTCCGTGAATAAGGATTTGCCATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑ttac > 1:623592/1‑233 (MQ=255) aTAAGGATTTGCCATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGAc > 1:37988/1‑235 (MQ=255) aaGGATTTGCCATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATg > 1:293951/1‑236 (MQ=255) aGGATTTGCCATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATgg < 1:421461/236‑1 (MQ=255) ttGCCATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAg > 1:485835/1‑236 (MQ=255) ttGCCATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAg < 1:24249/236‑1 (MQ=255) aaaaGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCtg > 2:253715/1‑211 (MQ=255) cgCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATc < 2:623589/235‑1 (MQ=255) caACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGt > 1:627398/1‑236 (MQ=255) aaCCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGt < 1:1230416/235‑1 (MQ=255) cAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCtgat > 2:814011/1‑205 (MQ=255) cAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCtgat < 1:814014/205‑1 (MQ=255) gCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCAt > 1:158525/1‑236 (MQ=255) tGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGCAGGACGACCATAAATTCCTGGGGGCAGCCGA‑ttaccc > 2:57178/1‑170 (MQ=255) gAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGGTGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑TGACCTGTGCGACATGGCCAAg > 1:384412/1‑189 (MQ=255) cTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGa < 1:1017789/174‑1 (MQ=255) ccTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGG > 2:611364‑M1/1‑233 (MQ=255) cTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCa > 2:246016/1‑157 (MQ=255) cTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGGAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGC > 1:144660‑M1/1‑233 (MQ=255) ctcCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTACGGCGCATTAGGCAt > 1:585847/1‑202 (MQ=255) aCGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGGGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCG < 2:672018‑M1/233‑1 (MQ=255) aCGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTT > 1:346381‑M1/1‑225 (MQ=255) gcgTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGCTGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCt > 2:61682/1‑151 (MQ=255) gcgTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATc < 2:37988/164‑1 (MQ=255) gcgTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTc > 1:1170245/1‑205 (MQ=255) gcgTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATgg > 1:1071976/1‑236 (MQ=255) gTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATggg < 1:820299/235‑1 (MQ=255) gCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGt > 1:971719/1‑172 (MQ=255) gCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGt < 2:971716/172‑1 (MQ=255) cGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAagcagc > 1:508311/1‑236 (MQ=255) gTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGcc > 1:1180015/1‑236 (MQ=255) tCCCCGTCGACGCGGCCTACGAGCATGGCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTG < 2:1135992‑M1/209‑1 (MQ=255) cacGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATc < 2:338731/145‑1 (MQ=255) cGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCCAGGCGCTGATGAACGCTATCGAGATCGCCAAGGGTAAGGCGCa > 2:346860/1‑168 (MQ=255) gTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCtgat < 2:6744/134‑1 (MQ=255) gTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGG < 2:1162109‑M1/193‑1 (MQ=255) gcgACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGG < 2:449620‑M1/191‑1 (MQ=255) cgGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGccc < 1:44297/224‑1 (MQ=255) aCGGGCATGTCTCGATCCTGATGGGCTACAGCAAGCGCCGGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCAT < 2:219195‑M1/174‑1 (MQ=255) gCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATggg < 2:485832/200‑1 (MQ=255) tctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGGAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCG > 2:265363‑M1/1‑184 (MQ=255) tctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGAGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCg < 1:38090/123‑1 (MQ=255) ctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCTCCAAGGGTAAGGCGCAGTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGT > 2:317875‑M1/1‑184 (MQ=255) ctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGTCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCgg > 2:623306/1‑213 (MQ=255) ctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGAttt > 1:1225450/1‑217 (MQ=255) ctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGAt > 2:737580/1‑215 (MQ=255) ctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCCGAGATTGCGCTGAAAAc > 1:224719/1‑236 (MQ=255) ctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGAAAAc > 1:644261/1‑236 (MQ=255) ctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTC > 1:561918‑M1/1‑185 (MQ=255) cGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGAGAGCGGCCTTGCGgc > 2:395848‑M1/1‑181 (MQ=255) aTCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGAAAACgggg < 1:253715/236‑1 (MQ=255) cTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGgcg > 2:66904/1‑135 (MQ=255) cTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGGCGCGA > 1:997571‑M1/1‑182 (MQ=255) ttatgGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCTGCCTT < 1:1180070‑M1/170‑1 (MQ=255) tGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGAt > 1:1193520/1‑216 (MQ=255) ggCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGAAAACGGGGGcc > 1:1211318/1‑230 (MQ=255) ggCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTG > 2:577681‑M1/1‑156 (MQ=255) cATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGCTCGCCAAg > 2:102030/1‑115 (MQ=255) cATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGA > 2:731271‑M1/1‑171 (MQ=255) cATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGG > 2:1024120‑M1/1‑152 (MQ=255) cATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGT > 2:255978‑M1/1‑150 (MQ=255) ccTGGTGCGCGAGGGCGATATCGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑TTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGAAAACGGGGGCCGCTTTGCGGCCCTt > 2:638845‑M2/1‑229 (MQ=255) cgggTGCGCGAGGGCGATATGGAGGACGACCAGAAATTCCTGGGGGCAGCCGAGT‑ACCTGTGCGACATGGCTCAGGCGCTGATGAACGGTATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATggg < 2:218605/164‑1 (MQ=255) gTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCG > 2:493182‑M1/1‑152 (MQ=255) tGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAagcagc > 1:1132701/1‑174 (MQ=255) gcgcGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCT > 2:607590‑M1/1‑146 (MQ=255) cgcgAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCaaa > 2:260614/1‑167 (MQ=255) cgcgAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGccc > 2:80892/1‑174 (MQ=255) cgcgAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGccc < 1:80892/174‑1 (MQ=255) gAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGaa > 2:617455/1‑196 (MQ=255) gAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGaa < 1:617458/196‑1 (MQ=255) gAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑TTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGAAAACGGGGGCCGCGTTGCGGCCCTttcgcgacgcaaggccg < 1:167104‑M2/236‑18 (MQ=255) gggCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCACCGGCg > 2:56528/1‑174 (MQ=255) atatGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGt < 1:960993/92‑1 (MQ=255) atatGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGt > 2:960990/1‑92 (MQ=255) aggaCGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGAAAACGGGGGCCGCTTTGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGc < 1:291892/236‑1 (MQ=255) cgacCATAAATTCCTGGGTGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGCGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGAttt > 2:1219659/1‑163 (MQ=255) cgacCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGg > 2:447186‑M1/1‑129 (MQ=255) cATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGTAGCGGCCGTGC > 2:606493‑M1/1‑124 (MQ=255) cATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGTAGC > 1:11518‑M1/1‑116 (MQ=255) cATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGag > 1:46967/1‑156 (MQ=255) cATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGAAAACGGGGGCCGCTTTGCGGCCCTTTCGCGACGCCAGGCCGCTCCCACaaaa > 1:978843/1‑226 (MQ=255) cATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGG < 1:815767‑M1/112‑1 (MQ=255) cATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGG > 2:815764‑M1/1‑112 (MQ=255) cATAAATTCCTGGGGGCAGCCGA‑TTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGAAAACGGGGGCCGCTTTGCGGCCCTttcgcgacgcaaggccg < 1:701109‑M2/215‑18 (MQ=255) cATAAATTCCTGGGGGCAGCCGA‑TTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGAAAACGGGGGCCGCTTTGCGGCCCTttcgcgaagcacggcc < 1:105693‑M2/214‑17 (MQ=255) tAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGAAAACGGGGGCCGCTTTGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCCGCTTgc < 2:1071973/233‑1 (MQ=255) aaTTCCTGGGGGCAGCCGA‑TTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGAAAACGGGGGCCGCTTTGCGGCCCTt > 1:445524‑M2/1‑194 (MQ=255) ggggCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGCGCGGCCTTGCGGCGCGAAGGGGCTg > 2:213515/1‑127 (MQ=255) ggggCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGAAAACGGGGGCCg > 1:697624/1‑174 (MQ=255) gggCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGGATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGAAAACGGGGGCCGCTTTGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCCGCTTGCGCTAACCACAAg < 2:1211315/234‑1 (MQ=255) cgatt‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGAAAACGGGGGCCGCTTTGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCCGCTTGCGCTAACca > 1:186972/5‑223 (MQ=255) | TCTCCTTCCGTGAATAAGGATTTGCCATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑T‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGAAAACGGGGGCCGCTTTGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCCGCTTGCGCTAACCACAAG > AE015451/335890‑336347 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |