Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AE015451 1,070,246:1 +GA 100% intergenic (+46/+96) aroP‑I → / ← PP_0928 aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger related‑protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154511,070,2461.G93.8% 280.8 / 10.6 80intergenic (+46/+96)aroP‑I/PP_0928aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger related‑protein
Reads supporting (aligned to +/- strand):  ref base . (4/1);  new base G (24/51);  total (28/52)
Fisher's exact test for biased strand distribution p-value = 4.84e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
*AE0154511,070,2462.A93.8% 281.2 / 10.2 80intergenic (+46/+96)aroP‑I/PP_0928aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger related‑protein
Reads supporting (aligned to +/- strand):  ref base . (4/1);  new base A (24/51);  total (28/52)
Fisher's exact test for biased strand distribution p-value = 4.84e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTTCTACAAAAGCCTGGGGCACCCGCTGACCAACTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GT‑‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAGGCGGCCAGCCAACGGCATACCCATGGTAAAAGCCGCCACA  >  AE015451/1070020‑1070471
                                                                                                                                                                                                                                       ||                                                                                                                                                                                                                                 
cTTCTACAAAAGCCTGGGGCACCCGCTGACCAACTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GT‑‑Gagatcgag                                                                                                                                                                                                                          <  1:1236129/236‑8 (MQ=255)
            ccTGGGGCACCCGCTGACCAACTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GT‑‑Ga                                                                                                                                                                                                                                 >  2:976342/1‑217 (MQ=255)
                        gCTGACCAACTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTc                                                                                                                                                                                                         <  1:1095966/231‑1 (MQ=255)
                         cTGACCAACTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAgcgc                                                                                                                                                                                                                         >  2:1127633/1‑214 (MQ=255)
                                       gtgCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAg                                                                                                                                                                                     <  1:408128/236‑1 (MQ=255)
                                            tGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGggcggc                                                                                                                                                                                                             >  2:443691/1‑207 (MQ=255)
                                               cGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGGATGCCGTTCGGTTGTCGTCGGC‑‑T‑‑GG‑‑Gaga                                                                                                                                                                                                                               >  2:201913/1‑182 (MQ=255)
                                                 ttCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGAtc                                                                                                                                                                                                     >  2:1100686/1‑210 (MQ=255)
                                                      cGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTca                                                                                                                                                                                                  >  1:488180/1‑208 (MQ=255)
                                                                   gTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCcgcgc                                                                                                                                                                                                                    <  1:720974/177‑1 (MQ=255)
                                                                                 gACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAg                                                                                                                                                                              >  2:424523/1‑201 (MQ=255)
                                                                                  aCGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCgcgggcg                                                                                                                                                                                                               <  1:1208258/167‑1 (MQ=255)
                                                                                         cgATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGccc                                                                                                                                     >  2:633089/1‑234 (MQ=255)
                                                                                            ttCGCAGCTCGGTAATGCTGCTCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGggcggc                                                                                                                                                                                                             <  2:892851/159‑1 (MQ=39)
                                                                                             tggcgtttgggTAAGGCTGATCCCGGCGTGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTcc                                                                                                                                           <  2:885245/215‑1 (MQ=255)
                                                                                              gtcATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGCTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTcc                                                                                                                                           <  1:663758/221‑1 (MQ=255)
                                                                                                     cGGTAATGCTGAGCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTc                                                                                                                                                                                                         <  2:948996/154‑1 (MQ=255)
                                                                                                          aTGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCa                                                                                                                  <  1:1127636/236‑1 (MQ=255)
                                                                                                          aTGCGGATCACGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTGCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCgcgggcg                                                                                                                                                                                                               <  2:166431/143‑1 (MQ=39)
                                                                                                             cTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgc                                                                                                               <  1:145190/236‑1 (MQ=255)
                                                                                                              tGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAAtgctg                                                                                                                           >  1:779949/1‑223 (MQ=255)
                                                                                                                   ccGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcacgc                                                                                                           <  1:17933/234‑1 (MQ=255)
                                                                                                                   ccGGCATGGACCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCg                                                                                                                     <  1:655001/224‑1 (MQ=255)
                                                                                                                    cGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGcaca                                                                                                        <  1:443694/236‑1 (MQ=255)
                                                                                                                     ggCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGt                                                                                                                                                    <  1:976345/191‑1 (MQ=255)
                                                                                                                      gCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCGCGGCAGGCCAAGGGCTGGTCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGa                                                                                                                                                                                           <  2:402600/151‑1 (MQ=38)
                                                                                                                       cATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtg                                                                                                     <  1:1207110/236‑1 (MQ=255)
                                                                                                                               gctgtgctgtGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCa                                                                                                                  >  1:67971/1‑215 (MQ=255)
                                                                                                                                ctgtgctgtgGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcac                                                                                                             <  1:521929/219‑1 (MQ=255)
                                                                                                                                  gtgctgtgGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATg                                                                                                                                                                                         <  2:1040431/141‑1 (MQ=255)
                                                                                                                                  gtgctgtgGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGAcgc                                                                                                                                                      <  2:403805/176‑1 (MQ=255)
                                                                                                                                   tgctgtgGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGGAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCcgcgc                                                                                                                                                                                                                    <  2:61152/113‑1 (MQ=39)
                                                                                                                                      tgtgGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGCGA‑‑GATCGAGCGCCGCGCGGGCGGCGCCCGAtcc                                                                                                                                                                                                    >  2:56058/1‑125 (MQ=38)
                                                                                                                                        tgggtggCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTg                                                                                                                                                          <  2:413576/166‑1 (MQ=255)
                                                                                                                                             ggCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGc                                                                                                                                                                            <  2:596880/143‑1 (MQ=255)
                                                                                                                                             ggCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAg                                                                                                                >  2:521926/1‑203 (MQ=255)
                                                                                                                                             ggCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTa                                                                                                >  2:438832/1‑219 (MQ=255)
                                                                                                                                                tttCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCt                                                                                                                                                                                                          >  1:211965/1‑110 (MQ=255)
                                                                                                                                                tttCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAg                                                                                             >  2:904494/1‑219 (MQ=255)
                                                                                                                                                 ttCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTGAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCAc                                                                           <  1:683788/236‑1 (MQ=255)
                                                                                                                                                 ttCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCAc                                                                           <  1:1235858/236‑1 (MQ=255)
                                                                                                                                                  tCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGGTTGTTCGTAAGGGGTATGCCGTTCGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGcc                                                                                                                                                             >  2:346950/1‑153 (MQ=38)
                                                                                                                                                  tCAAGCGGAAGACGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTGAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTc                                                                                                                                  <  1:211445/180‑1 (MQ=39)
                                                                                                                                                    aaGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCg                                                                         <  1:1100689/235‑1 (MQ=255)
                                                                                                                                                     aGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcacgca                                                                                                          <  2:712345/201‑1 (MQ=255)
                                                                                                                                                     aGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcac                                                                                                             <  1:1065397/198‑1 (MQ=255)
                                                                                                                                                       cTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCt                                                                                                                                                           >  2:594712/1‑150 (MQ=255)
                                                                                                                                                            gaagGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCCCGCGGGCGGCGCTCGAgct                                                                                                                                                                                                    >  2:1060116/1‑104 (MQ=37)
                                                                                                                                                                gCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtg                                                                                                     <  1:1063635/195‑1 (MQ=255)
                                                                                                                                                                       aGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGc                                                                                                                                       <  1:415845/154‑1 (MQ=255)
                                                                                                                                                                       aGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGc                                                                                                                                       >  2:415842/1‑154 (MQ=255)
                                                                                                                                                                         gCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTcc                                                                                                                                           <  2:1258282/148‑1 (MQ=255)
                                                                                                                                                                               ggCTGATCGGGTGTGTTCGTACGGGGTGTGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGcaca                                                                                                        <  2:135997/177‑1 (MQ=39)
                                                                                                                                                                                gCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcac                                                                                                             <  2:1012220/171‑1 (MQ=255)
                                                                                                                                                                                 cTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCGGT‑‑GC‑‑GATCGAGCGCCGCCCGCGCGTCGCCCGATCTCACAg                                                                                                                                                                                               >  1:7798/1‑88 (MQ=255)
                                                                                                                                                                                 cTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGCCTGGCGGCCCTGAcgcg                                                                                                                                                     >  2:1034930/1‑130 (MQ=38)
                                                                                                                                                                                   gATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCg                                                                         <  1:1116973/204‑1 (MQ=255)
                                                                                                                                                                                   gATCGGGTTTGTTCGTAAGGGGCATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGCTCTCACAGGCGATGAAAGCCTAAAgg                                                                                                                                                                             <  1:1280918/104‑1 (MQ=25)
                                                                                                                                                                                    ttCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTc                                                                                                                       <  1:1052536/156‑1 (MQ=255)
                                                                                                                                                                                     tCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCg                                                                         <  1:495285/202‑1 (MQ=255)
                                                                                                                                                                                     tCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCg                                                                         <  2:672977/202‑1 (MQ=255)
                                                                                                                                                                                       gggTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTa                                                                                                >  2:1272829/1‑177 (MQ=255)
                                                                                                                                                                                        ggTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCCCAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATa                                                               <  1:114862/209‑1 (MQ=255)
                                                                                                                                                                                              ttCGTAAGGGGTAGGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAGGCGgcc                                    <  1:201913/230‑1 (MQ=255)
                                                                                                                                                                                               tCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTc                                                                                                                                                                     <  1:694172/100‑1 (MQ=25)
                                                                                                                                                                                               tCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAGGCGGCCAGCCAAc                             <  1:904497/236‑1 (MQ=255)
                                                                                                                                                                                                 gTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCTAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTGCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcac                                                                                                             <  1:523381/154‑1 (MQ=38)
                                                                                                                                                                                                 gTAAGGGGTATGCCGTTAGGCTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAGGCGGCCAGCCAACg                            <  1:1269665/235‑1 (MQ=255)
                                                                                                                                                                                                    aGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTAc                                                                                               <  2:1005064/165‑1 (MQ=255)
                                                                                                                                                                                                    aGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAGGCGGCCAGCCAACGGCAt                        <  1:1188452/236‑1 (MQ=255)
                                                                                                                                                                                                      gggTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAGGCGGCCAGCCAACGGCATAc                      >  1:556243/1‑236 (MQ=255)
                                                                                                                                                                                                           tGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGCTCTCACAGGCGATGAAAGGCTAAAGGCATGCGTCTGGCGGACCTGAcg                                                                                                                                                       >  1:473739/1‑102 (MQ=25)
                                                                                                                                                                                                              cGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCg                                                                         <  1:1272832/177‑1 (MQ=255)
                                                                                                                                                                                                              cGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAGGCGgccagc                                 >  1:230922/1‑217 (MQ=255)
                                                                                                                                                                                                                  aGGTTTGCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAGGCGgccag                                  <  1:424526/212‑1 (MQ=255)
                                                                                                                                                                                                                   ggTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAGGCGgccagcca                               >  1:313169/1‑214 (MQ=255)
                                                                                                                                                                                                                   ggTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCAGCCAAGCACGCAAAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAGGCGGCCAGCCAACGGCATACCCATGGTAAAAgc         >  1:1215821/1‑236 (MQ=255)
                                                                                                                                                                                                                    gTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCCTGCCCCTCACTGCTGCCTCCGCCAAGCCCGcaca                                                                                                        >  2:165412/1‑140 (MQ=37)
                                                                                                                                                                                                                       ttCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcac                                                                                                             <  2:373026/132‑1 (MQ=255)
                                                                                                                                                                                                                           gcgcC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAGGCGGCCAGCCAACGGCATACCCATGGTAAAAGCCGCcaca  <  1:839037/235‑1 (MQ=255)
                                                                                                                                                                                                                                       ||                                                                                                                                                                                                                                 
CTTCTACAAAAGCCTGGGGCACCCGCTGACCAACTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GT‑‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAGGCGGCCAGCCAACGGCATACCCATGGTAAAAGCCGCCACA  >  AE015451/1070020‑1070471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑