Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	1,932,638:1	+C	100%	intergenic (‑105/+45)	rluA ← / ← minE	23S rRNA (746) and tRNA (32) pseudouridine synthase/Cell division topological specificity factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	1,932,638	1	.	C	94.9%	212.4 / 4.2	59	intergenic (‑105/+45)	rluA/minE	23S rRNA (746) and tRNA (32) pseudouridine synthase/Cell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base C (33/23);  total (35/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGCAGACGGGTGATCAGGCAGTCCTTGTTGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑C‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATG  >  AE015451/1932406‑1932863                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                    tGCAGACGGGTGCTCAGGCAGTCCTTGTTGCCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑c‑cc                                                                                                                                                                                                                                 <  1:183168/236‑2 (MQ=255)                 ggCAGTCCTTGTTGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCa                                                                                                                                                                                                                    <  2:1121466/233‑1 (MQ=255)                        cTTGTTGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGcccc                                                                                                                                                                                                          >  1:1096311/1‑236 (MQ=255)                         ttgttgTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑c                                                                                                                                                                                                                                    >  2:1095948/1‑210 (MQ=255)                                ccTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCa                                                                                                                                                                                                                    <  2:858730/218‑1 (MQ=255)                                    ggCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCAGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACt                                                                                                                                                                                              >  1:612368/1‑236 (MQ=255)                                      cACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACt                                                                                                                                                                                              <  2:1184045/234‑1 (MQ=255)                                       aCGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑c‑ccgcg                                                                                                                                                                                                                              >  2:791730/1‑197 (MQ=255)                                       aCGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑c‑ccgcg                                                                                                                                                                                                                              <  1:791733/201‑5 (MQ=255)                                            cAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACtt                                                                                                                                                                                             >  1:825000/1‑228 (MQ=255)                                                                gTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGACGGGCTGCAAGGCAGCCCCAAATTGTCGACTAc                                                                                                                                                                                            <  2:1202700/210‑1 (MQ=255)                                                                      ttgttgATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGtt                                                                                                                                                               >  2:1088426/1‑233 (MQ=255)                                                                            aTCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGc                                                                                                                                                                                                                     >  1:978727/1‑173 (MQ=255)                                                                                 cAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCa                                                                                                                                                                                                                    >  2:612559/1‑169 (MQ=255)                                                                                 cAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCa                                                                                                                                                                                                                    <  1:612562/169‑1 (MQ=255)                                                                                 cAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCACGGCAGCCCCa                                                                                                                                                                                                         >  1:97325/1‑180 (MQ=255)                                                                                       ggcggcGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATg                                                                                                                                                                 <  1:882736/214‑1 (MQ=255)                                                                                          ggcgTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGa                                                                                                                                                                                       >  1:1221644/1‑189 (MQ=255)                                                                                           gcgTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGAt                                                                                                                                                                                      >  1:78009/1‑189 (MQ=255)                                                                                              tCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑CTC‑GCGAAGGGCTGCAAGGCAGccc                                                                                                                                                                                                           >  1:723729/1‑165 (MQ=255)                                                                                              tCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGCCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGACGGGCTGCAAGGCAGCCCCAAATTGt                                                                                                                                                                                                   <  1:641830/173‑1 (MQ=255)                                                                                                 tCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTg                                                                                                                                                                    >  2:971974/1‑201 (MQ=255)                                                                                                            cTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGCTATGCGTCCCCTGTAGCAGCGGGCTTG‑CAC‑GCGAAGGGCTGCAAGGCCGccc                                                                                                                                                                                                           >  2:641827/1‑151 (MQ=255)                                                                                                            cTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑c‑ccgcg                                                                                                                                                                                                                              <  2:198633/132‑5 (MQ=255)                                                                                                              ggACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGttt                                                                                                                                >  2:971741/1‑224 (MQ=255)                                                                                                               gACATTCGACAGCAGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGata                                                                                                                    >  1:641705/1‑235 (MQ=255)                                                                                                                       aCAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatc                                                                                                            >  2:639382/1‑235 (MQ=255)                                                                                                                        cAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑c‑ccgcg                                                                                                                                                                                                                              <  1:432777/120‑5 (MQ=255)                                                                                                                           cGGCGTTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCg                                                                                                                                                                            <  2:783636/167‑1 (MQ=255)                                                                                                                                             gAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTGCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAGTTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGata                                                                                                                    >  2:1179561/1‑205 (MQ=255)                                                                                                                                              aaaaTAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAtt                                                                                     >  1:746176/1‑235 (MQ=255)                                                                                                                                                    gCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGat                                                                                                                     <  1:1215932/197‑1 (MQ=255)                                                                                                                                                         aaaCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGc                                                                                  <  2:1095906/227‑1 (MQ=255)                                                                                                                                                          aaCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGc                                                                                                                          <  1:601981/186‑1 (MQ=255)                                                                                                                                                             gCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGTTCGATATGCACATCATCGttt                                                                                                     >  1:524139/1‑203 (MQ=255)                                                                                                                                                             gCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCca                                                                      <  1:878226/235‑1 (MQ=255)                                                                                                                                                                    ccGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTcac                                                                                          >  1:1119547/1‑208 (MQ=255)                                                                                                                                                                          tGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCa                                                                                                                                                                         >  1:1146903/1‑123 (MQ=255)                                                                                                                                                                          tGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGtt                                                                                                                                                               >  2:908536/1‑133 (MQ=255)                                                                                                                                                                                   tCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGc                                               <  1:1088429/236‑1 (MQ=255)                                                                                                                                                                                            aGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAg                                         <  2:855441/233‑1 (MQ=255)                                                                                                                                                                                            aGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTcc                                      >  1:855444/1‑236 (MQ=255)                                                                                                                                                                                               ccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCgg                                    <  1:1271851/235‑1 (MQ=255)                                                                                                                                                                                                      tACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGt                                                                                              <  1:491982/170‑1 (MQ=255)                                                                                                                                                                                                      tACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGt                                                                                              >  2:491979/1‑170 (MQ=255)                                                                                                                                                                                                          aaGGATATGCTTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATg                                                                                                                                                                 <  1:1207688/99‑1 (MQ=255)                                                                                                                                                                                                          aaGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATGCGGCAGAGTGATg                                                                                                                                                                 >  2:1207685/1‑99 (MQ=37)                                                                                                                                                                                                                atGCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTcc                                      >  2:304103/1‑216 (MQ=255)                                                                                                                                                                                                                  gCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGat                                                                                                                     >  2:549083/1‑135 (MQ=255)                                                                                                                                                                                                                  gCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGc                                                                                                    <  2:554356/152‑1 (MQ=255)                                                                                                                                                                                                                  gCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGt                                                                 >  1:166346/1‑187 (MQ=255)                                                                                                                                                                                                                  gCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAg                                             >  1:119152/1‑207 (MQ=255)                                                                                                                                                                                                                  gCGTCCCCTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACGACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTa                                          >  2:403191/1‑210 (MQ=255)                                                                                                                                                                                                                        ccTGTAGGAGCGGGCTTGCC‑C‑GCGAAGCGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAgc                                                     <  2:773718/193‑1 (MQ=255)                                                                                                                                                                                                                         cTGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCcagc                                                                    <  2:746050/177‑1 (MQ=255)                                                                                                                                                                                                                          tGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTcc                                                                                                                             <  2:32011/119‑1 (MQ=255)                                                                                                                                                                                                                          tGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTcc                                                                                                                             >  1:32011/1‑119 (MQ=255)                                                                                                                                                                                                                          tGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTgcgc         >  1:312008/1‑235 (MQ=255)                                                                                                                                                                                                                          tGTAGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGcc        >  1:747217/1‑236 (MQ=255)                                                                                                                                                                                                                             aGGAGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGTGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGcccc      >  1:1210900/1‑233 (MQ=255)                                                                                                                                                                                                                                aGCGGGCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACgatg  <  1:293838/236‑1 (MQ=255)                                                                                                                                                                                                                                    ggCTTG‑C‑CGGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATg                                                                                                                                                                 <  2:989913/73‑1 (MQ=255)                                                                                                                                                                                                                                     gCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTcc                                                                                                                             <  2:1149763/108‑1 (MQ=255)                                                                                                                                                                                                                                     gCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTcc                                                                                                                             >  1:1149766/1‑108 (MQ=255)                                                                                                                                                                                                                                     gCTTGCC‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGc                                                                                  >  2:160837/1‑151 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                    TGCAGACGGGTGATCAGGCAGTCCTTGTTGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑C‑C‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATG  >  AE015451/1932406‑1932863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑