New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 2532679	64 (0.930)	3 (0.050)	3/322	NT	3.9%	coding (451/531 nt)	PP_2223	conserved protein of unknown function
	?	AE015451	= 2533246	86 (1.320)					coding (101/366 nt)	mrpG	putative Multicomponent potassium‑proton antiporter, subunit G
Rejected: Frequency below/above cutoff threshold.

TAGTCGATGACAAGCAGCGCCTTGAACTGAAAGTCGGCGACCACTTTCGTGCTGCCGTATTGCCCAGCCGCGAAGGTGGCAAGCTGGAAATGCGCTTTCGCGACATCAAGTTGAATGTGTATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/2532443‑2532679 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aggcctggTACGCTTGAAAGACTACTTCCAGCGCATGCACCCGCCAGCGCTGGCCTCGACCCTTGGTGCCTGGTGCGTTGCACTGGCCTCGATCATCTACTTTTCGTGGCTCAAGGGCACCCCTGTGCTGCACGCTTGGCTGATTCCGATCCTGCTGTCGATTACCGTGCCGATAACCACGCTGTTGCTGGCGCGGGCGGCGCTATTTCGCAAGCGCATGTCGAAAGAGCCGGTGCCTGAAGA  <  AE015451/2533246‑2533012                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          TAGTCGACGACAAGCAGCGCCTTGACCTGAAAGTCGGCGACCACTTTCGTGCTGCCGTATTGCCCAGCCGCGAAGGTGGCAAGCTGGAAATGCGCTTTCGCGACATCAAGTTGAATGTGTATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCC                                                                                                                                                                                                                                                <  1:118059/234‑1                                       GACCACTTTCGTGCTGCCGTATTGCCCAGCCGCGAAGGTGGCAAGCTGGAAATGCGCTTTCGCGACATCAAGTTGAATGTGTATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCT                                                                                                                                                                                                                                               >  1:614943/1‑197                                                          TATTGCCCAGCCGCGAAGGTGGCAAGCTGGAAATGCGCTTTCGCGACATCAAGTTGAATGTGTATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGG                                                                                                                                                                                                                                                  >  2:438916/1‑175                                                                                                                TGAATGTGTATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCC                                                                                                                                                                                                                                                <  2:463564/123‑1                                                                                                                                     ATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGGGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGTACGCTTGAAAGACTACTTCCAGCGCATGCACCCGCCAGCGCTGGCCTCGACCCTTGGTGCCTGGTGCGTTGCACTGGCCTCGATCATCTACTTTTCGTGGCTCAAGGGCACCCCTGT                                                                                                                       <  1:884277/223‑1                                                                                                                                                                                                           ATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGTACGCTTGAAAGACTACT                                                                                                                                                                                                                           <  1:1009279/53‑1                                                                                                                                                                                                           ATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGTACGCTTGAAAGACTACT                                                                                                                                                                                                                           >  2:1009276/1‑53                                                                                                                                                                                                                                        GCCTGGTACGCTTGAAAGGCTCCTTCCAGCGCATGCACCCGCCAGCGCTGGCCTCGACCCTTGGTGCCTGGTGCGTTGCACTGGCCTCGATCATCTACTTTTCGTGGCTCAAGGGCACCCCTGTGCTGCACGCTTGGCTGATTCCGATCCT                                                                                            <  2:1279771/151‑1                                                                                                                                                                                                                                             GTACGCTTGAAAGACTACTTCCAGCGCATGCACCCGCCAGCGCTGGCCTCGACCCTTGGTGCCTGGTGCGTTGCACTGGCCTCGATCATCTACTTTTCGTGGCTCAAGGGCACCCCTGTGCTGCACGCTTGGCTGATTCCGATCCTGCTGTCGATTACCGTGCCGATAACCACGCTGTTGCTGGCGCGGGCGGCGCTATTTCGCAAGCGCATGTCGAAAGAGCCGGTGCCTGAAGA  >  1:1097131/1‑236                                                                                                                                                                                                                                             GTACGCTTGAAAGACTACTTCCAGCGCATGCACCCGCCAGCGCTGGCCTCGACCCTTGGTGCCTGGTGCGTTGCACTGGCCTCGATCATCTACTTTTCGTGGCTCAAGGGCACCCCTGTGCTGCACGCTTGGCTGATTCCGATCCTGCTGTCGATTACCGTGCCGATAACCACGCTGTTGCTGGCGCGGGCGGCGCTATTTCGCAAGCGCATGTCGAAAGAGCCGGTGCCTGAAGA  >  1:583682/1‑236                                                                                                                                                                                                                                             GTACGCTTGAAAGACTACTTCCAGCGCATGCACCCGCCAGCGCTGGCCTCGACCCTTGGTGCCTGGTGCGTTGCACTGGCCTCGATCATCTACTTTTCGTGGCTCAAGGGCACCCCTGTGCTGCACGCTTGGCTGATTCCGATCCTGCTGTCGATTACCGTGCCGATAACCACGCTGTTGCTGGCGCGGGCGGCGCTATTTCGCAAGCGCATGTCGAAAGAGCCGGTGCCTGAAG   >  1:487926/1‑235                                                                                                                                                                                                                                             GTACGCTTGAAAGACTACTTCCAGCGCATGCACCCGCCAGCGCTGGCCTCGACCCTTGGTGCCTGGTGCGTTGCACTGGCCTCGATCATCTACTTTTCGTGGCTCAAGGGCACCCCTGTGCTGCACGCTTGGCTGATTCCGATCCTGCTGTCGATTACCGTGCCGATAACCACGCTGTTGCTGGC                                                     >  2:309240/1‑185                                                                                                                                                                                                                                             GTACGCTTGAAAGAATACTTC                                                                                                                                                                                                                         >  2:70070/1‑21                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          TAGTCGATGACAAGCAGCGCCTTGAACTGAAAGTCGGCGACCACTTTCGTGCTGCCGTATTGCCCAGCCGCGAAGGTGGCAAGCTGGAAATGCGCTTTCGCGACATCAAGTTGAATGTGTATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/2532443‑2532679 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aggcctggTACGCTTGAAAGACTACTTCCAGCGCATGCACCCGCCAGCGCTGGCCTCGACCCTTGGTGCCTGGTGCGTTGCACTGGCCTCGATCATCTACTTTTCGTGGCTCAAGGGCACCCCTGTGCTGCACGCTTGGCTGATTCCGATCCTGCTGTCGATTACCGTGCCGATAACCACGCTGTTGCTGGCGCGGGCGGCGCTATTTCGCAAGCGCATGTCGAAAGAGCCGGTGCCTGAAGA  <  AE015451/2533246‑2533012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.